PAPSS2 (NM_001015880) Human Untagged Clone

CAT#: SC302005

PAPSS2 (untagged)-Human 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (PAPSS2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAPSS2
• Synonyms: ATPSK2; BCYM4; SK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC302005 representing NM_001015880.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCGGGGATCAAGAAGCAAAAGACGGAGAACCAGCAGAAATCCACCAATGTAGTCTATCAGGCCCAC
  CATGTGAGCAGGAATAAGAGAGGGCAAGTGGTTGGAACAAGGGGTGGGTTCCGAGGATGTACCGTGTGG
  CTAACAGGTCTCTCTGGTGCTGGAAAAACAACGATAAGTTTTGCCCTGGAGGAGTACCTTGTCTCCCAT
  GCCATCCCTTGTTACTCCCTGGATGGGGACAATGTCCGTCATGGCCTTAACAGAAATCTCGGATTCTCT
  CCTGGGGACAGAGAGGAAAATATCCGCCGGATTGCTGAGGTGGCTAAGCTGTTTGCTGATGCTGGTCTG
  GTCTGCATTACCAGCTTTATTTCTCCATTCGCAAAGGATCGTGAGAATGCCCGCAAAATACATGAATCA
  GCAGGGCTGCCATTCTTTGAAATATTTGTAGATGCACCTCTAAATATTTGTGAAAGCAGAGACGTAAAA
  GGCCTCTATAAAAGGGCCAGAGCTGGGGAGATTAAAGGATTTACAGGTATTGATTCTGATTATGAGAAA
  CCTGAAACTCCTGAGCGTGTGCTTAAAACCAATTTGTCCACAGTGAGTGACTGTGTCCACCAGGTAGTG
  GAACTTCTGCAAGAGCAGAACATTGTACCCTATACTATAATCAAAGATATCCACGAACTCTTTGTGCCG
  GAAAACAAACTTGACCACGTCCGAGCTGAGGCTGAAACTCTCCCTTCATTATCAATTACTAAGCTGGAT
  CTCCAGTGGGTCCAGGTTTTGAGCGAAGGCTGGGCCACTCCCCTCAAAGGTTTCATGCGGGAGAAGGAG
  TACTTACAGGTTATGCACTTTGACACCCTGCTAGATGGCATGGCCCTTCCTGATGGCGTGATCAACATG
  AGCATCCCCATTGTACTGCCCGTCTCTGCAGAGGATAAGACACGGCTGGAAGGGTGCAGCAAGTTTGTC
  CTGGCACATGGTGGACGGAGGGTAGCTATCTTACGAGACGCTGAATTCTATGAACACAGAAAAGAGGAA
  CGCTGTTCCCGTGTTTGGGGGACAACATGTACAAAACACCCCCATATCAAAATGGTGATGGAAAGTGGG
  GACTGGCTGGTTGGTGGAGACCTTCAGGTGCTGGAGAAAATAAGATGGAATGATGGGCTGGACCAATAC
  CGTCTGACACCTCTGGAGCTCAAACAGAAATGTAAAGAAATGAATGCTGATGCGGTGTTTGCATTCCAG
  TTGCGCAATCCTGTCCACAATGGCCATGCCCTGTTGATGCAGGACACTCGCCGCAGGCTCCTAGAGAGG
  GGCTACAAGCACCCGGTCCTCCTACTACACCCTCTGGGCGGCTGGACCAAGGATGACGATGTGCCTCTA
  GACTGGCGGATGAAGCAGCACGCGGCTGTGCTCGAGGAAGGGGTCCTGGATCCCAAGTCAACCATTGTT
  GCCATCTTTCCGTCTCCCATGTTATATGCTGGCCCCACAGAGGTCCAGTGGCACTGCAGGTCCCGGATG
  ATTGCGGGTGCCAATTTCTACATTGTGGGGAGGGACCCTGCAGGAATGCCCCATCCTGAAACCAAGAAG
  GATCTGTATGAACCCACTCATGGGGGCAAGGTCTTGAGCATGGCCCCTGGCCTCACCTCTGTGGAAATC
  ATTCCATTCCGAGTGGCTGCCTACAACAAAGCCAAAAAAGCCATGGACTTCTATGATCCAGCAAGGCAC
  AATGAGTTTGACTTCATCTCAGGAACTCGAATGAGGAAGCTCGCCCGGGAAGGAGAGAATCCCCCAGAT
  GGCTTCATGGCCCCCAAAGCATGGAAGGTCCTGACAGATTATTACAGGTCCCTGGAGAAGAACTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001015880
• Insert Size: 1860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001015880.1
• RefSeq Size: 3874 bp
• RefSeq ORF: 1860 bp
• Locus ID: 9060
• UniProt ID: O95340
• Cytogenetics: 10q23.2-q23.31
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism, Selenoamino acid metabolism, Sulfur metabolism
• MW: 70 kDa
• Gene Summary: Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) includes an alternate in-frame segment, compared to variant 1, resulting in a longer protein (isoform 2), compared to isoform 1. Isoform 2 has also been named PAPS synthase 2b.