PIWIL3 (NM_001008496) Human Untagged Clone

CAT#: SC301309

PIWIL3 (untagged)-Human piwi-like 3 (Drosophila) (PIWIL3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIWIL3
• Synonyms: HIWI3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC301309 representing NM_001008496.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCTGGTAGGGCAAGGACTCGCGCCCGAGGCAGAGCCCGCCGCAGGGAGAGCTACCAACAAGAGGCA
  CCTGGGGGACCCAGAGCACCTGGATCAGCTACAACCCAGGAGCCCCCTCAGTTGCAGTCGACACCCCGG
  CCGCTGCAGGAGGAAGTCCCAGTGGTTAGACCTCTGCAGCCAAGAGCAGCAAGAGGAGGAGCAGGAGGA
  GGAGCACAGTCTCAAGGGGTGAAGGAACCTGGACCTGAGGCTGGGTTGCATACAGCGCCCTTGCAGGAG
  AGAAGGATTGGTGGAGTTTTTCAAGACCTGGTGGTGAACACCAGGCAAGATATGAAGCATGTTAAAGAC
  TCAAAAACAGGTTCAGAGGGTACAGTGGTACAGCTACTCGCCAACCACTTCCGAGTGATATCTCGTCCT
  CAGTGGGTTGCATATAAATACAACGTTGACTACAAACCAGACATAGAAGATGGAAATCTCCGTACAATT
  TTACTTGATCAACATAGAAGGAAATTTGGAGAGCGCCATATATTTGATGGAAACTCTTTATTATTATCT
  CGGCCACTAAAAGAGCGGAGAGTGGAATGGTTGAGCACAACCAAAGACAAAAACATCGTGAAGATTACA
  GTTGAGTTTTCCAAAGAACTCACGCCCACGTCGCCAGATTGCCTACGCTATTACAACATTCTCTTTAGA
  AGAACTTTCAAGCTGCTGGATTTTGAACAAGTTGGTCGCAACTATTATACCAAAAAGAAGGCCATTCAG
  TTATACCGTCATGGTACCAGTTTGGAAATCTGGCTTGGTTATGTTACTTCTGTTCTTCAATACGAAAAC
  AGCATTACCCTCTGTGCCGATGTGAGCCACAAACTGCTCCGAATAGAAACTGCTTATGATTTCATAAAG
  AGAACATCTGCCCAGGCCCAGACAGGAAACATCCGAGAGGAAGTAACTAATAAATTAATTGGATCAATT
  GTTCTGACAAAATACAACAACAAAACCTACAGAGTAGATGATATTGATTGGAAGCAGAATCCTGAAGAC
  ACATTTAACAAATCAGATGGCAGCAAAATCACCTATATAGACTACTACAGGCAGCAACATAAAGAAATT
  GTCACAGTGAAGAAACAGCCACTTTTGGTCAGCCAGGGCAGATGGAAAAAGGGCCTAACGGGTACACAA
  CGTGAACCTATCCTGCTGATTCCTCAGCTGTGCCACATGACAGGTCTAACAGATGAAATATGTAAAGAT
  TATAGCATTGTGAAAGAATTGGCTAAACATACAAGATTGAGTCCAAGAAGAAGGCATCATACATTAAAA
  GAATTCATCAATACTCTACAAGATAATAAAAAAGTACGAGAGTTACTTCAACTCTGGGATTTGAAATTT
  GATACCAATTTTTTGTCCGTCCCGGGAAGAGTTTTGAAAAACGCAAACATCGTGCAAGGCAGAAGAATG
  GTTAAAGCCAATTCACAAGGAGACTGGTCAAGAGAAATAAGAGAATTACCCTTACTTAATGCAATGCCA
  CTACATAGTTGGCTCATACTCTATAGCAGGAGCAGTCACAGAGAAGCCATGTCCTTAAAGGGTCATCTA
  CAGAGTGTCACAGCCCCCATGGGCATAACTATGAAACCAGCAGAAATGATTGAAGTAGATGGTGATGCT
  AACTCCTATATAGACACATTACGGAAATATACTAGACCAACACTGCAGATGGGAATGTCATGTTTGCTG
  GTTTTCAAGGTGATTTGTATCCTGCCCAATGATGACAAACGTAGATATGACAGCATAAAAAGATACCTA
  TGTACCAAATGCCCAATTCCAAGCCAGTGTGTGGTGAAAAAGACCTTAGAAAAAGTCCAGGCAAGGACC
  ATCGTCACCAAGATTGCCCAGCAGATGAATTGCAAGATGGGAGGAGCCCTCTGGAAGGTGGAGACAGAC
  GTACAAAGAACAATGTTCGTTGGCATTGATTGTTTCCACGATATCGTAAATCGACAGAAATCAATAGCA
  GGATTTGTTGCAAGTACCAATGCTGAATTAACAAAGTGGTACTCTCAATGTGTCATCCAGAAAACAGGA
  GAAGAGCTTGTGAAAGAGCTGGAGATCTGCTTGAAAGCTGCCCTGGATGTCTGGTGTAAAAACGAATCA
  TCGATGCCACATTCTGTTATTGTGTATCGGGATGGAGTGGGAGATGGTCAGCTTCAAGCATTGCTTGAC
  CATGAAGCGAAAAAGATGTCGACCTACTTAAAAACCATCTCTCCTAACAATTTCACTCTAGCTTTCATT
  GTGGTGAAGAAACGAATAAACACTAGATTTTTTCTTAAACATGGAAGCAATTTTCAAAATCCACCTCCA
  GGAACAGTTATTGATGTAGAGTTGACTAGGAATGAATGGTATGACTTTTTTATTGTGAGTCAGTCTGTG
  CAAGATGGGACTGTTACCCCCACTCATTATAACGTCATCTATGACACGATTGGCTTGAGCCCAGATACA
  GTACAGCGTTTAACATATTGTCTATGCCACATGTATTATAATTTGCCAGGCATCATCCGAGTTCCAGCG
  CCTTGCCACTATGCCCACAAGCTGGCTTACCTCGTGGGGCAGTCCATTCACCAGGAACCGAATCGTTCC
  TTGTCAACTCGTCTCTTTTACCTTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008496
• Insert Size: 2649 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008496.3
• RefSeq Size: 3508 bp
• RefSeq ORF: 2649 bp
• Locus ID: 440822
• UniProt ID: Q7Z3Z3
• Cytogenetics: 22q11.23
• Protein Pathways: Dorso-ventral axis formation
• MW: 101.1 kDa
• Gene Summary: This gene encodes a member of the PIWI subfamily of Argonaute family proteins. This subfamily of proteins contains a PAZ domain, found in proteins involved in RNA-mediated gene silencing, and a C-terminal Piwi domain. The encoded protein is thought to function in maintenance of germline cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein (isoform 1).