Laminin 5 (LAMB3) (NM_000228) Human Untagged Clone

CAT#: SC300029

LAMB3 (untagged)-Human laminin, beta 3 (LAMB3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LAMB3
• Synonyms: AI1A; BM600-125KDA; LAM5; LAMNB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000228 edited
  GGCTGTTGTTTAAAAACCTGGAGCCGGGAGGGGAGACCCCCACATTCAAGAGGAGCTTTC
  AGGCGATCTGGAGAAAGAACGGCAGAACACACAGCAAGGAAAGGTCCTTTCTGGGGATCA
  CCCCATTGGCTGAAGATGAGACCATTCTTCCTCTTGTGTTTTGCCCTGCCTGGCCTCCTG
  CATGCCCAACAAGCCTGCTCCCGTGGGGCCTGCTATCCACCTGTTGGGGACCTGCTTGTT
  GGGAGGACCCGGTTTCTCCGAGCTTCATCTACCTGTGGACTGACCAAGCCTGAGACCTAC
  TGCACCCAGTATGGCGAGTGGCAGATGAAATGCTGCAAGTGTGACTCCAGGCAGCCTCAC
  AACTACTACAGTCACCGAGTAGAGAATGTGGCTTCATCCTCCGGCCCCATGCGCTGGTGG
  CAGTCCCAGAATGATGTGAACCCTGTCTCTCTGCAGCTGGACCTGGACAGGAGATTCCAG
  CTTCAAGAAGTCATGATGGAGTTCCAGGGGCCCATGCCCGCCGGCATGCTGATTGAGCGC
  TCCTCAGACTTCGGTAAGACCTGGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCC
  ACCTTCCCTCGGGTCCGCCAGGGTCGGCCTCAGAGCTGGCAGGATGTTCGGTGCCAGTCC
  CTGCCTCAGAGGCCTAATGCACGCCTAAATGGGGGGAAGGTCCAACTTAACCTTATGGAT
  TTAGTGTCTGGGATTCCAGCAACTCAAAGTCAAAAAATTCAAGAGGTGGGGGAGATCACA
  AACTTGAGAGTCAATTTCACCAGGCTGGCCCCTGTGCCCCAAAGGGGCTACCACCCTCCC
  AGCGCCTACTATGCTGTGTCCCAGCTCCGTCTGCAGGGGAGCTGCTTCTGTCACGGCCAT
  GCTGATCGCTGCGCACCCAAGCCTGGGGCCTCTGCAGGCCCCTCCACCGCTGTGCAGGTC
  CACGATGTCTGTGTCTGCCAGCACAACACTGCCGGCCCAAATTGTGAGCGCTGTGCACCC
  TTCTACAACAACCGGCCCTGGAGACCGGCGGAGGGCCAGGACGCCCATGAATGCCAAAGG
  TGCGACTGCAATGGGCACTCAGAGACATGTCACTTTGACCCCGCTGTGTTTGCCGCCAGC
  CAGGGGGCATATGGAGGTGTGTGTGACAATTGCCGGGACCACACCGAAGGCAAGAACTGT
  GAGCGGTGTCAGCTGCACTATTTCCGGAACCGGCGCCCGGGAGCTTCCATTCAGGAGACC
  TGCATCTCCTGCGAGTGTGATCCGGATGGGGCAGTGCCAGGGGCTCCCTGTGACCCAGTG
  ACCGGGCAGTGTGTGTGCAAGGAGCATGTGCAGGGAGAGCGCTGTGACCTATGCAAGCCG
  GGCTTCACTGGACTCACCTACGCCAACCCGCAGGGCTGCCACCGCTGTGACTGCAACATC
  CTGGGGTCCCGGAGGGACATGCCGTGTGACGAGGAGAGTGGGCGCTGCCTTTGTCTGCCC
  AACGTGGTGGGTCCCAAATGTGACCAGTGTGCTCCCTACCACTGGAAGCTGGCCAGTGGC
  CAGGGCTGTGAACCGTGTGCCTGCGACCCGCACAACTCCCTCAGCCCACAGTGCAACCAG
  TTCACAGGGCAGTGCCCCTGTCGGGAAGGCTTTGGTGGCCTGATGTGCAGCGCTGCAGCC
  ATCCGCCAGTGTCCAGACCGGACCTATGGAGACATGGCCACAGGATGCCGAGCCTGTGAC
  TGTGATTTCCGGGGAACAGAGGGCCCGGGCTGCGACAAGGCATCAGGCCGCTGCCTCTGC
  CGCCCTGGCTTGACCGGGCCCCGCTGTGACCAGTGCCAGCGAGGCTACTGCAATCGCTAC
  CCGGTGTGCGTGGCCTGCCACCCTTGCTTCCAGACCTATGATGCGGACCTCCGGGAGCAG
  GCCCTGCGCTTTGGTAGACTCCGCAATGCCACCGCCAGCCTGTGGTCAGGGCCTGGGCTG
  GAGGACCGTGGCCTGGCCTCCCGGATCCTAGATGCAAAGAGTAAGATTGAGCAGATCCGA
  GCAGTTCTCAGCAGCCCCGCAGTCACAGAGCAGGAGGTGGCTCAGGTGGCCAGTGCCATC
  CTCTCCCTCAGGCGAACTCTCCAGGGCCTGCAGCTGGATCTGCCCCTGGAGGAGGAGACG
  TTGTCCCTTCCGAGAGACCTGGAGAGTCTTGACAGAAGCTTCAATGGTCTCCTTACTATG
  TATCAGAGGAAGAGGGAGCAGTTTGAAAAAATAAGCAGTGCTGATCCTTCAGGAGCCTTC
  CGGATGCTGAGCACAGCCTACGAGCAGTCAGCCCAGGCTGCTCAGCAGGTCTCCGACAGC
  TCGCGCCTTTTGGACCAGCTCAGGGACAGCCGGAGAGAGGCAGAGAGGCTGGTGCGGCAG
  GCGGGAGGAGGAGGAGGCACCGGCAGCCCCAAGCTTGTGGCCCTGAGGCTGGAGATGTCT
  TCGTTGCCTGACCTGACACCCACCTTCAACAAGCTCTGTGGCAACTCCAGGCAGATGGCT
  TGCACCCCAATATCATGCCCTGGTGAGCTATGTCCCCAAGACAATGGCACAGCCTGTGGC
  TCCCGCTGCAGGGGTGTCCTTCCCAGGGCCGGTGGGGCCTTCTTGATGGCGGGGCAGGTG
  GCTGAGCAGCTGCGGGGCTTCAATGCCCAGCTCCAGCGGACCAGGCAGATGATTAGGGCA
  GCCGAGGAATCTGCCTCACAGATTCAATCCAGTGCCCAGCGCTTGGAGACCCAGGTGAGC
  GCCAGCCGCTCCCAGATGGAGGAAGATGTCAGACGCACACGGCTCCTAATCCAGCAGGTC
  CGGGACTTCCTAACAGACCCCGACACTGATGCAGCCACTATCCAGGAGGTCAGCGAGGCC
  GTGCTGGCCCTGTGGCTGCCCACAGACTCAGCTACTGTTCTGCAGAAGATGAATGAGATC
  CAGGCCATTGCAGCCAGGCTCCCCAACGTGGACTTGGTGCTGTCCCAGACCAAGCAGGAC
  ATTGCGCGTGCCCGCCGGTTGCAGGCTGAGGCTGAGGAAGCCAGGAGCCGAGCCCATGCA
  GTGGAGGGCCAGGTGGAAGATGTGGTTGGGAACCTGCGGCAGGGGACAGTGGCACTGCAG
  GAAGCTCAGGACACCATGCAAGGCACCAGCCGCTCCCTTCGGCTTATCCAGGACAGGGTT
  GCTGAGGTTCAGCAGGTACTGCGGCCAGCAGAAAAGCTGGTGACAAGCATGACCAAGCAG
  CTGGGTGACTTCTGGACACGGATGGAGGAGCTCCGCCACCAAGCCCGGCAGCAGGGGGCA
  GAGGCAGTCCAGGCCCAGCAGCTTGCGGAAGGTGCCAGCGAGCAGGCATTGAGTGCCCAA
  GAGGGATTTGAGAGAATAAAACAAAAGTATGCTGAGTTGAAGGACCGGTTGGGTCAGAGT
  TCCATGCTGGGTGAGCAGGGTGCCCGGATCCAGAGTGTGAAGACAGAGGCAGAGGAGCTG
  TTTGGGGAGACCATGGAGATGATGGACAGGATGAAAGACATGGAGTTGGAGCTGCTGCGG
  GGCAGCCAGGCCATCATGCTGCGCTCAGCGGACCTGACAGGACTGGAGAAGCGTGTGGAG
  CAGATCCGTGACCACATCAATGGGCGCGTGCTCTACTATGCCACCTGCAAGTGATGCTAC
  AGCTTCCAGCCCGTTGCCCCACTCATCTGCCGCCTTTGCTTTTGGTTGGGGGCAGATTGG
  GTTGGAATGCTTTCCATCTCCAGGAGACTTTCATGCAGCCTAAAGTACAGCCTGGACCAC
  CCCTGGTGTGTAGCTAGTAAGATTACCCTGAGCTGCAGCTGAGCCTGAGCCAATGGGACA
  GTTACACTTGACAGACAAAGATGGTGGAGATTGGCATGCCATTGAAACTAAGAGCTCTCA
  AGTCAAGGAAGCTGGGCTGGGCAGTATCCCCCGCCTTTAGTTCTCCACTGGGGAGGAATC
  CTGGACCAAGCACAAAAACTTAACAAAAGTGATGTAAAAATGAAAAGCCAAATAAAAATC
  TTTGGAAAAGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000228
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000228.2, NP_000219.2
• RefSeq Size: 4093 bp
• RefSeq ORF: 3519 bp
• Locus ID: 3914
• UniProt ID: Q13751
• Cytogenetics: 1q32.2
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• Gene Summary: The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1, also known as B3A) encodes the same protein as variants 2 and 3.