GLUR3 (GRIA3) (NM_007325) Human Untagged Clone

CAT#: SC127844

GRIA3 (untagged)-Human glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLUR3
• Synonyms: GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC; MRX94; MRXSW
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127844 sequence for NM_007325 edited (data generated by NextGen Sequencing)
  ATGGCCAGGCAGAAGAAAATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTAGTCCTG
  GGGCTTTTGGGTCATTCTCACGGAGGATTCCCCAACACCATCAGCATAGGTGGACTTTTC
  ATGAGAAACACAGTGCAGGAGCACAGCGCTTTCCGCTTTGCCGTGCAGTTATACAACACC
  AACCAGAACACCACCGAGAAGCCCTTCCATTTGAATTACCACGTAGATCACTTGGATTCC
  TCCAATAGTTTTTCCGTGACAAATGCTTTCTGCTCCCAGTTCTCGAGAGGGGTGTATGCC
  ATCTTTGGATTCTATGACCAGATGTCAATGAACACCCTGACCTCCTTCTGTGGGGCCCTG
  CACACATCCTTTGTTACGCCTAGCTTCCCCACTGACGCAGATGTGCAGTTTGTCATCCAG
  ATGCGCCCAGCCTTGAAGGGCGCTATTCTGAGTCTTCTGGGTCATTACAAGTGGGAGAAG
  TTTGTGTACCTCTATGACACAGAACGAGGATTTTCCATCCTCCAAGCGATTATGGAAGCA
  GCAGTGCAAAACAACTGGCAAGTAACAGCAAGGTCTGTGGGAAACATAAAGGACGTCCAA
  GAATTCAGGCGCATCATTGAAGAAATGGACAGGAGGCAGGAAAAGCGATACTTGATTGAC
  TGCGAAGTCGAAAGGATTAACACAATTTTGGAACAGGTTGTGATCCTAGGGAAACACTCA
  AGAGGTTATCACTACATGCTCGCTAACCTGGGTTTTACTGATATTTTACTGGAAAGAGTC
  ATGCATGGGGGAGCCAACATTACAGGTTTCCAGATTGTCAACAATGAAAACCCTATGGTT
  CAGCAGTTCATACAGCGCTGGGTGAGGCTGGATGAAAGGGAATTCCCTGAAGCCAAGAAT
  GCACCACTAAAGTATACATCTGCATTGACACACGACGCAATACTGGTCATAGCAGAAGCT
  TTCCGCTACCTGAGGAGGCAGCGAGTAGATGTGTCCCGGAGAGGAAGTGCTGGAGACTGC
  TTAGCAAATCCTGCTGTGCCCTGGAGTCAAGGAATTGATATTGAGAGAGCTCTGAAAATG
  GTGCAAGTACAAGGAATGACTGGAAATATTCAATTTGACACTTATGGACGTAGGACAAAT
  TATACCATCGATGTGTATGAAATGAAAGTCAGTGGCTCTCGAAAAGCTGGCTACTGGAAT
  GAGTATGAAAGGTTTGTGCCTTTCTCAGATCAGCAAATCAGCAATGACAGTGCATCCTCA
  GAGAATCGGACCATAGTAGTGACTACCATTCTGGAATCACCATATGTAATGTACAAGAAG
  AACCATGAGCAACTGGAAGGAAATGAACGATATGAAGGCTATTGTGTAGACCTAGCCTAT
  GAAATAGCCAAACATGTAAGGATCAAATACAAATTGTCCATCGTTGGTGACGGGAAATAT
  GGTGCAAGGGATCCAGAGACTAAAATATGGAACGGCATGGTTGGGGAACTTGTCTATGGG
  AGAGCTGATATAGCTGTTGCTCCACTCACTATAACATTGGTCCGTGAAGAAGTCATAGAT
  TTTTCAAAGCCATTCATGAGCCTGGGCATCTCCATCATGATAAAGAAGCCTCAGAAATCA
  AAACCAGGCGTATTCTCATTTCTGGATCCCCTGGCTTATGAAATCTGGATGTGCATTGTC
  TTTGCTTACATTGGAGTCAGCGTAGTTCTTTTCCTAGTCAGCAGGTTCAGTCCTTATGAA
  TGGCACTTGGAAGACAACAATGAAGAACCTCGTGACCCACAAAGTCCTCCTGATCCTCCA
  AATGAATTTGGAATATTTAACAGTCTTTGGTTTTCCTTGGGTGCCTTTATGCAGCAAGGA
  TGTGATATTTCTCCAAGATCACTCTCCGGGCGCATTGTTGGAGGGGTTTGGTGGTTCTTC
  ACCCTGATCATAATTTCTTCCTATACTGCCAATCTCGCTGCTTTCCTGACTGTGGAGAGG
  ATGGTTTCTCCCATAGAGAGTGCTGAAGACTTAGCTAAACAGACTGAAATTGCATATGGG
  ACCCTGGACTCCGGTTCAACAAAAGAATTTTTCAGAAGATCCAAAATTGCTGTGTACGAG
  AAAATGTGGTCTTACATGAAATCAGCGGAGCCATCTGTGTTTACCAAAACAACAGCAGAC
  GGAGTGGCCCGAGTGCGAAAGTCCAAGGGAAAGTTCGCCTTCCTGCTGGAGTCAACCATG
  AATGAGTACATTGAGCAGAGAAAACCATGTGATACGATGAAAGTTGGTGGAAATCTGGAT
  TCCAAAGGCTATGGTGTGGCAACCCCTAAAGGCTCAGCATTARGAACGCCTGTAAACCTT
  GCAGTATTGAAACTCAGTGAACAAGGCATCTTAGACAAGCTGAAAAACAAATGGTGGTAC
  GATAAGGGGGAATGTGGAGCCAAGGACTCCGGGAGTAAGGACAAGACCAGCGCTCTGAGC
  CTGAGCAATGTGGCAGGCGTTTTCTATATACTTGTCGGAGGTCTGGGGCTGGCCATGATG
  GTGGCTTTGATAGAATTCTGTTACAAATCACGGGCAGAGTCCAAACGCATGAAACTCACA
  AAGAACACCCAAAACTTTAAGCCTGCTCCTGCCACCAACACTCAGAATTATGCTACATAC
  AGAGAAGGCTACAACGTGTATGGAACAGAGAGTGTTAAGATCTAG
  
  Clone variation with respect to NM_007325.4
  2323 g=>r
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007325 unedited
  GCGAATTCGGCACGAGGCTCTTGTCAGCTTCGTTTTAGGCGTAGCATGGCCAGGCAGAAG
  AAAATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTAGTCCTGGGGCTTTTGGGTCAT
  TCTCACGGAGGATTCCCCAACACCATCAGCATAGGTGGACTTTTCATGAGAAACACAGTG
  CAGGAGCACAGCGCTTTCCGCTTTGCCGTGCAGTTATACAACACCAACCAGAACACCACC
  GAGAAGCCCTTCCATTTGAATTACCACGTAGATCACTTGGATTCCTCCAATAGTTTTTCC
  GTGACAAATGCTTTCTGCTCCCAGTTCTCGAGAGGGGTGTATGCCATCTTTGGATTCTAT
  GACCAGATGTCAATGAACACCCTGACCTCCTTCTGTGGGGCCCTGCACACATCCTTTGTT
  ACGCCTAGCTTCCCCACTGACGCAGATGTGCAGTTTGTCATCCAGATGCGCCCAGCCTTG
  AAGGGCGCTATTCTGAGTCTTCTGGGTCATTACAAGTGGGAGAAGTTTGTGTACCTCTAT
  GACACAGAACGAGGATTTTCCATCCTCCAAGCGATTATGGAAGCAGCAGTGCANAACAAC
  TGGCAAGTAACAGCAGGGTCTGTGGGAAACATANAGGACGTCCAAGAATTCAGGCGCATC
  ATTGAAGAAATGGACAGGAGGCAGGAANAGCGATACTTGATTGACTGGGAAGTCGAAAGG
  ATAAACACATTTGGACAGGGTGGTGATCTAGGGAACATCCAAGAGTTATACTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007325 unedited
  NGGGCCTGAGGTATTTCATCACTTAAGTATTAAAACTTTGTACAATACCTCATGGCAATG
  AGGCTCTGTAGTTTATGCCCACACATGCAAATTACGCTTTTATAGCCATTAGTTTGCTTG
  GCAATTTTTCTGTTCACTGAAGTACAGCTTTAACAACAAAAAGAAAAGAATTGGAAAAAA
  AATCCTCAAGTGACTACTGCCAAACTCCCATAGCTTGAAGTTAGTAGCAGAAATTTGAAG
  CAATCAACTTTTTTTTAAACATGCAAGTTTTTTTTAAAAAATAACAAACCAATTTACCCG
  CCTCTTCATCTCAGTGACGTTTCCTCTCTGAAAAACTGATTTGAGTTAATAAACATACAT
  TTTTCTTTATACAGTGCCTATGAAAACATTTGTGTGTAGGTTGGTTGGTTTGTTTTATAC
  AACTTCATGTTCACACAGCGTGAGCTTCCCGCGCTGGGACATTATAGCATTCATAAGTTG
  AAAATCACAGTTTCATGAAAACAAAACAAAACAAAAACACAACAGCTTTCTTTTTTTGTT
  CAATACCATTTGAAATTTGACAAGTGAGTTGTTTGCTTATGACACCATTTTTGGAATGAA
  AGTTTATAGTACACTTTTCTGGAAACATTTCGTTGGAACGATACTTCTTCCTTTCTTTTC
  TGAAGACAAGAGCAAATATGCAGCGAGTCAACTAAGCATCTAAGGGTCACACTTGGGAAA
  TGAATGCGAGCTCCTAGTTTTTGCAGTCTGGAAACTTCCCAATGCACGTTATCGATGAGA
  GCAAACTACTGAACTACAGGAAGCATACGTCGTTATATAGGAAGTAATTAGTGAGTGGAG
  ACTATTTCTTCGACTGTTCTGATGATGCCAACGGCAGGATGCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007325
• Insert Size: 4500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007325.2, NP_015564.2
• RefSeq Size: 3148 bp
• RefSeq ORF: 2685 bp
• Locus ID: 2892
• UniProt ID: P42263
• Cytogenetics: Xq25
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Long-term depression, Neuroactive ligand-receptor interaction
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes isoform 1 (also known as flip isoform). RNA editing (AGA->GGA) changes Arg775Gly.