YAP1 (NM_006106) Human Untagged Clone

CAT#: SC127739

YAP1 (untagged)-Human Yes-associated protein 1 (YAP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: YAP1
• Synonyms: COB1; YAP; YAP2; YAP65; YKI
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127739 sequence for NM_006106 edited (data generated by NextGen Sequencing)
  ATGGATCCCGGGCAGCAGCCGCCGCCTCAACCGGCCCCCCAGGGCCAAGGGCAGCCGCCT
  TCGCAGCCCCCGCAGGGGCAGGGCCCGCCGTCCGGACCCGGGCAACCGGCACCCGCGGCG
  ACCCAGGCGGCGCCGCAGGCACCCCCCGCCGGGCATCAGATCGTGCACGTCCGCGGGGAC
  TCGGAGACCGACCTGGAGGCGCTCTTCAACGCCGTCATGAACCCCAAGACGGCCAACGTG
  CCCCAGACCGTGCCCATGAGGCTCCGGAAGCTGCCCGACTCCTTCTTCAAGCCGCCGGAG
  CCCAAATCCCACTCCCGACAGGCCAGTACTGATGCAGGCACTGCAGGAGCCCTGACTCCA
  CAGCATGTTCGAGCTCATTCCTCTCCAGCTTCTCTGCAGTTGGGAGCTGTTTCTCCTGGG
  ACACTGACCCCCACTGGAGTAGTCTCTGGCCCAGCAGCTACACCCACAGCTCAGCATCTT
  CGACAGTCTTCTTTTGAGATACCTGATGATGTACCTCTGCCAGCAGGTTGGGAGATGGCA
  AAGACATCTTCTGGTCAGAGATACTTCTTAAATCACATCGATCAGACAACAACATGGCAG
  GACCCCAGGAAGGCCATGCTGTCCCAGATGAACGTCACAGCCCCCACCAGTCCACCAGTG
  CAGCAGAATATGATGAACTCGGCTTCAGCCATGAACCAGAGAATCAGTCAGAGTGCTCCA
  GTGAAACAGCCACCACCCCTGGCTCCCCAGAGCCCACAGGGAGGCGTCATGGGTGGCAGC
  AACTCCAACCAGCAGCAACAGATGCGACTGCAGCAACTGCAGATGGAGAAGGAGAGGCTG
  CGGCTGAAACAGCAAGAACTGCTTCGGCAGGAGTTAGCCCTGCGTAGCCAGTTACCAACA
  CTGGAGCAGGATGGTGGGACTCAAAATCCAGTGTCTTCTCCCGGGATGTCTCAGGAATTG
  AGAACAATGACGACCAATAGCTCAGATCCTTTCCTTAACAGTGGCACCTATCACTCTCGA
  GATGAGAGTACAGACAGTGGACTAAGCATGAGCAGCTACAGTGTCCCTCGAACCCCAGAT
  GACTTCCTGAACAGTGTGGATGAGATGGATACAGGTGATACTATCAACCAAAGCACCCTG
  CCCTCACAGCAGAACCGTTTCCCAGACTACCTTGAAGCCATTCCTGGGACAAATGTGGAC
  CTTGGAACACTGGAAGGAGATGGAATGAACATAGAAGGAGAGGAGCTGATGCCAAGTCTG
  CAGGAAGCTTTGAGTTCTGACATCCTTAATGACATGGAGTCTGTTTTGGCTGCCACCAAG
  CTAGATAAAGAAAGCTTTCTTACATGGTTATAG
  
  Clone variation with respect to NM_006106.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006106 unedited
  CGCGAATTCCGCACCAGGGGCGCAGGGCGGGGGCGGAGGCGCCGGGGCGGGGGATGCGGG
  GCCGCGGCGCAACCCCCCGGCCCTGAGAGCGAGGACAGCGCCGCCCGGCCCGCAGCCGTC
  GCCGCTTCTCCACCTCGGCCCGTGGAGCCGGGGCGTCCGGGCGTANCCCTCGCTCGCCTG
  GGTCAGGGGGTGCGCGTCGGGGGAGGCAGAAGCCATGGATCCCGGGCAGCAGCCGCCGCC
  TCAACCGGCCCCCCAGGGCCAAGGGCAGCCGCCTTCGCAGCCCCCGCAGGGGCAGGGCCC
  GCCGTCCGGACCCGGGCAACCGGCACCCGCGGCGACCCAGGCGGCGCCGCAGGCACCCCC
  CGCCGGGCATCAGATCGTGCACGTCCGCGGGGACTCGGAGACCGACCTGGAGGCGCTCTT
  CAACGCCGTCATGAACCCCAAGACGGCCAACGTGCCCCAGACCGTGCCCATGAGGCTCCG
  GAAGCTGCCCGACTCCTTCTTCAAGCCGCCGGAGCCCANATCCCACTCCCGACAGGCCAG
  TACTGATGCAGGCACTGCAGGAGCCCTGACTCCACAGCATGTTCGAGCTCATTCCTCTCC
  AGCTTCTCTGCAGGTGGGAGCTGTTTCTCCTGGGACACTGACCCCCACTGGAGTAGTCTC
  TGGCCCAGCAGCTACACCCACAGCTCAGCATCTTCGACAGTCTTCTTTTGAGAACCTGAT
  GATGTACCTCTGCCAGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006106 unedited
  AGTAATTCGGGTTNATGAACCACATCAGGGACAAATTGAATACATAAGAAAAAAAGTAAA
  TTTTATGTAAGTACCTAACATATGAGCATGCTCTTACATCTAAAACAAAAAATAAAAAGG
  TAACATTGGTACTATATATATATATTTGACAAGTGTGCATTAAAGAATTCTCTAATATAA
  AACATTTAAAATGTGGAGAATACTTTTTTATTATACAGAAAACAATTGTTATGATAGGCA
  CACCCACAATTCTTATAACAACATGCTTGCGAGGATAAAATCCACCTGAGCACTCATTTC
  ACAGATGTACCAACGCTAGAAAAGTGTTAAGCACTGAATATTGCCACCCACTTTTGCAAT
  GTTTGAGTTTCAACACTGATTGGTATGAATTCTGAATTACACAATTAATTACTGTTATTT
  TTCAGTCTTTCTGCCATGTTCCATATAGAAGGCATGTATTTAATATGAATACTTAACACA
  GCAACATTATTTGTAGCAAAGTCACTTCCCTGTGTTCATTTTTCCTTTAAAGGCACTACA
  TTTAGAAAAGGTAATACTACAAAAAGTGCTTTGGAAAATCTGAAACTCCAAATCAATGTG
  CTTCATCCACCAAAAGTGTATCTTAGAAGCCCTGACTGAAAATACCCACATGTAAAAGTG
  GTTAATTTTAAAGATTTAAAATTGGGTTATTGGTTAAGCAAGTTTAGAATTCCCGTTAAA
  ATTTTATTCCAAGGTTTCGCATGTAACCAGAAACGGTGAAGACCCTGTTCCTATGGCATA
  AGACGTTTATCAGGTCTCCAGCAGGGCTTTGTTTCCAGTCAAGAGGGAAAGCACTCTGAG
  ACTTCTTCAGGTCAATACGTTGAGGTAATTACGACCAATCCTGGCCAGCGGTACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006106
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006106.2, NP_006097.1
• RefSeq Size: 5128 bp
• RefSeq ORF: 1365 bp
• Locus ID: 10413
• Cytogenetics: 11q22.1
• Domains: WW
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site and lacks two alternate exons in the coding region, compared to variant 9. The encoded protein (isoform 2) represents the YAP1-1alpha isoform described in Figure 3 of PMID: 22939869. It is shorter, compared to isoform 9. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.