GPR85 (NM_018970) Human Untagged Clone

CAT#: SC127481

GPR85 (untagged)-Human G protein-coupled receptor 85 (GPR85), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR85
• Synonyms: SREB; SREB2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127481 sequence for NM_018970 edited (data generated by NextGen Sequencing)
  ATGGCGAACTATAGCCATGCAGCTGACAACATTTTGCAAAATCTCTCGCCTCTAACAGCC
  TTTCTGAAACTGACTTCCTTGGGTTTCATAATAGGAGTCAGCGTGGTGGGCAACCTCCTG
  ATCTCCATTTTGCTAGTGAAAGATAAGACCTTGCATAGAGCACCTTACTACTTCCTGTTG
  GATCTTTGCTGTTCAGATATCCTCAGATCTGCAATTTGTTTCCCATTTGTGTTCAACTCT
  GTCAAAAATGGCTCTACCTGGACTTATGGGACTCTGACTTGCAAAGTGATTGCCTTTCTG
  GGGGTTTTGTCCTGTTTCCACACTGCTTTCATGCTCTTCTGCATCAGTGTCACCAGATAC
  TTAGCTATCGCCCATCACCGCTTCTATACAAAGAGGCTGACCTTTTGGACGTGTCTGGCT
  GTGATCTGTATGGTGTGGACTCTGTCTGTGGCCATGGCATTTCCCCCGGTTTTAGACGTG
  GGCACTTACTCATTCATTAGGGAGGAAGATCAATGCACCTTCCAACACCGCTCCTTCAGG
  GCTAATGATTCCTTAGGATTTATGCTGCTTCTTGCTCTCATCCTCCTAGCCACACAGCTT
  GTCTACCTCAAGCTGATATTTTTCGTCCACGATCGAAGAAAAATGAAGCCAGTCCAGTTT
  GTAGCAGCAGTCAGCCAGAACTGGACTTTTCATGGTCCTGGAGCCAGTGGCCAGGCAGCT
  GCCAATTGGCTAGCAGGATTTGGAAGGGGTCCCACACCACCCACCTTGCTGGGCATCAGG
  CAAAATGCAAACACCACAGGCAGAAGAAGGCTATTGGTCTTAGACGAGTTCAAAATGGAG
  AAAAGAATCAGCAGAATGTTCTATATAATGACTTTTCTGTTTCTAACCTTGTGGGGCCCC
  TACCTGGTGGCCTGTTATTGGAGAGTTTTTGCAAGAGGGCCTGTAGTACCAGGGGGATTT
  CTAACAGCTGCTGTCTGGATGAGTTTTGCCCAAGCAGGAATCAATCCTTTTGTCTGCATT
  TTCTCAAACAGGGAGCTGAGGCGCTGTTTCAGCACAACCCTTCTTTACTGCAGAAAATCC
  AGGTTACCAAGGGAACCTTACTGTGTTATATGA
  
  Clone variation with respect to NM_018970.6
  252 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018970 unedited
  TTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTTACTGAGAGCTCC
  ATTTCTGGAAAGCCTTACAAGACTGAGGAATATCAGACTGCGAATCACCGGGAACGGTTC
  CTTTGCAGCACAGAAGCAATCTCTCTCCCCATCTTCGCATATTCTGATGGCAAAACAAGT
  GGAAGAAAAGAGGAAGCATGACTGCAGATCAGATCAGTTCTCTTTGTGGATTATATTTTC
  AGTAAAATGTATGGATCTATCTTTTCCTTGTTCTTATATCTAGATCATGAGACTTGACTG
  AGGCTGTATCCTTATCCTCCATCCATCTATGGCGAACTATAGCCATGCAGCTGACAACAT
  TTTGCAAAATCTCTCGCCTCTAACAGCCTTTCTGAAACTGACTTCCTTGGGTTTCATAAT
  AGGAGTCAGCGTGGTGGGCAACCTCCTGATCTCCATTTTGCTAGTGAAAGATAAGACCTT
  GCATAGAGCACCTTACTACTTCCTGTTGGATCTTTGCTGTTCAGATATCCTCAGATCTGC
  AATTTGTTTCCCATTTGTGTTCAACTCTGTCAAAAATGGCTCTACCTGNACTTATGGGAC
  TCTGACTTGCAAAGTGATTGCCTTTCTGGNGGTTTTGTCCTGTTTCCACACTGCTTTCAT
  GCTCTTCTGCATCAGTGTCACCAGATACTTAGCTATCGCCCATCACCGCTTCTATACAAG
  AGGCTGACCTTNTGGACGTGTCTGGCTGTGATCTGTATGGTGTGGACTCTGTCTGTGGCC
  ATGGCATTTCCCCCGTTTTAGACGTGGCACTTACTCATTCATAGGNANGAAAGATCATGC
  ACCTTNCCACACCGNTNCCTTAGGGCTAATGATCCCTTAAGATNATGCTGCTNCTGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018970 unedited
  TTATGGACCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCATTGAAACA
  TTTCATTGATTTATTTTCCTTATTTATAGGGATATTCTCTTTACAGAAGTACTATAGCAA
  TATGGAATTCTGTGTTATTAGATTCCTAATCTTAATTATCAAATACCAAAAAAAAATAAA
  CTTCACAAAAACCAACCAATAAACAAAACAACAGAAAGCAAACAAAAATAATAACTTTTT
  CTAGTTTTGTCATAATGTTAGTGTAAACCATTCTTCTTACAAGTTGTATATTTAGAACCC
  CAAATTTCTATGGTACTATGCTCCAAATTAAAGTGTGATGTTTATATTAAAATTCTGAGC
  CAATTAACTAAGCAGGTACACATCTCTAAATACTACTGGCATTTAAGAATAATAGAGGGA
  TGATTATAATAAACTACCAAATAATGCTTTTACAGATAATATTATGTCAGTTTATCAGCA
  TTATGTTGATTATCATTCATACATTTCCAGTCTGGAATATAGTAGCTACAAGAATCAAAA
  AGCAAAACCATAATAAAGCTAGTCTTCCTAGTTTGTGCTTGCCTCCCAAATAAAAAAACG
  TTGATATGTGCCAAAAATGTAAAACTTATTTCAAGAAATTAACCAGCTACACCGGTAAAT
  TTTGCAAATGCCACTTTATTTTGAAGTCACAATCTGTATCTAAAGATGAGACTACAGACT
  TTAATGTATTTTTAAATACCAGAACTCATATTTAAGCTTTCCAAGTTTAACCTGAATTAT
  TTAAATTACCATCCCCTGAATGGGCAGAAAGTTAAACAGACTGGGCCCTTCAAAAATAAA
  TGTTTACAAATGGGGAGGAGGCCTACTAAGGTGGGGGTCCCCTTGACGCTGACCTAAAAT
  AAAAAAAACAACTGGCCGGTTTTAAAATGTAAAAGGGGGCACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018970
• Insert Size: 3780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018970.3, NP_061843.2
• RefSeq Size: 4919 bp
• RefSeq ORF: 1113 bp
• Locus ID: 54329
• UniProt ID: P60893
• Cytogenetics: 7q31.1
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: Members of the G protein-coupled receptor (GPCR) family, such as GPR85, have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli, such as neurotransmitters, hormones, or light, induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins, or G proteins (Matsumoto et al., 2000 [PubMed 10833454]).[supplied by OMIM, Aug 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All variants (1-4) encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.