DDX6 (AK021715) Human Untagged Clone

CAT#: SC127271

(untagged)-Human cDNA FLJ11653 fis, clone HEMBA1004538

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX6
• Synonyms: HLR2; P54; RCK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK021715, the custom clone sequence may differ by one or more nucleotides
  
  
  TTTTTTTTTTTTTTCTTTCTTTTTTGGGCCCTCATAATAAGCATTGTTACTATTGGAAGTTGTTTTCACA
  TTCTTTCCAATATTAAATATGTATTTTTTTAAGTAATGATAATATTTTCCAGTGGCTCATTTGGATGAGA
  ACTACCCTCTATTTTTAATATTAAAACTACATCCAACTCATCATTTAGCCTTTGGTTGTACAGTTGTGTA
  ATGGGCTATGGACTGTTACACACCTTACCACCTCTAGGCCTATGTTTTTTCTTTCCCCATATATTCTGAT
  GGGGATAAATACTGTTTTGCCTCTCCCATAGGAATGGAATACATTTATTCTAAAATGATCTTTCACAGAA
  GTAAGAGAGAGGGAAACCTAAATATACCTCTAAATTGTTTGAAGTTGGTCCCAGCAGCATAAAATGGGTT
  GGCCCCAAAGGGTTGGAGGGTGGGCTTGGTTATCAGTATTTGTTTTCAGAATGAGATGGGAGCATCTTTC
  CTTTGCCACGTGCTTTGTGCTTGATAACATCATGCTTGGTTCAAACGACAACTCAGCACAAAGCCTTGAG
  TATAAATTGTTGGAATCAAAACATCTCATTCTGATGACGTGGTTTAATTTTTTAATTTTTTTTTTTAATA
  GGGGTGGGAGGGAGGGTACTTTGCCCCAAAAGGGAGGGTGTCTGCACTAAGGATTTAGAAACACTTTGGA
  AGCTCATAACCTCATCAGAAACTGCCTTTAGCCACACTCCTGACCTTCTAGATGAGTAACAAAAAAATGA
  AATAAGTTCTTGGAAATTAAGCCATTTATTTTAATTTGCTATTTTTTTCAATGTTCTAGGTATCTTTAAA
  TATTGTGGAATCATTTTCCTGCCAGATACCTTTATCAAAATTATTGGCCTCATGAGAGCTGAAGTAAGTC
  AGCTTTTTGGTGAACTTTAGTGGACTTCTGTGAGATTGTAGTTGTACTTTGTATCTCTAAATCTAAAGAT
  AGTTTTTTAAAACTCCCAAAGAAAATCTGCTCTCCTTTCTGATCTAAAAACTCATCTTTGGGGTAAAGAG
  TTAAGTGTCCAAAGGTTGTCACAGTTCATGAGGTCAGAGGGAGCTAGCCTGGCACCTGGACTCTGCCCAT
  CCACAGCTGACAGATTCCAACAGAAGTGTATTTAAATTCTCCAGTAGACAATGCTGGGTAAGGGAGGGGG
  TAGGGCTGGGTTATTAAGATACAGGCTGCTGTATTTTACATTGGTTATGGGGGAAGGGGAGCCTGGAGAA
  AACAAAGTCACTATTCCCTTTTTTGAAACAGGAAAAAAAATTATTTTTTGTTCAGTAAAAATGGTAGAGA
  ATTCCAATGTCCCTAGCCACAAGGGACCAGTTCCACTGAGAAGTGAACAGTGGGAACTCAAAATTTCAGA
  AACATTGGGGGAAGGGAAAATTGGCTTTCTCTTAATTGGCAGATGTTCCAGTGGGGGGGGGGGGGGGCTC
  TGTTTTTGTTGGGATGTGTTATGTTGTATGTACGCATATATGGACCGGAGTCTGCTGAGTTTATAAGGTT
  CCAAAAATATGGTAAAATCTTGGTTTTTGTTAATTTATCTCAATAAAAGCCCACTGGAACTCC
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK021715 unedited
  GCACGAGGTAGTTGTACTTTGTATCTCTAAATCTAAAGATAGTTTTTTAAAACTCCCAAA
  GAAAATCTGCTCTCCTTTCTGATCTAAAAACTCATCTTTGGGGTAAAGAGTTAAGTGTCC
  AAAGGTTGTCACAGTTCATGAGGTCAGAGGGAGCTAGCCTGGCACCTGGACTCTGCCCAT
  CCACAGCTGACAGATTCCAACAGAAGTGTATTTAAATTCTCCAGTAGACAATGCTGGGTA
  AGGGAGGGGGTAGGGCTGGGTTATTAAGATACAGGCTGCTGTATTTTACATTGGTTGTGG
  GGGAAGGGGAGCCTGGAGAAAACAAAGTCACTATTCCCTTTTTTGAAACAGGAAAAAAAA
  TTATTTTTTGTTCAGTAAAAATGGTAGAGAATTCCAATGTCCCTAGCCACAAGGGACCAG
  TTCCACTGAGAAGTGAACAGTGGGAACTCAAAATTTCAGAAACATTGGGGGAAGGGAAAA
  TTGGCTTTCTCTTAATTGGCAGATGTTCCAGTGGGGCGGGGGGGCTCTGTTTTTGTTGGG
  ATGTGTTATGTTGTATGTACGCATATATGGACCGGAGTCTGCTGAGTTTATAAGGTTCCA
  AAAATATGGTAAAATCTTGGTTTTTGTTAATTTATCTCAATAAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: AK021715
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK021715.1
• RefSeq Size: 1603 bp
• RefSeq ORF: 1603 bp
• Locus ID: 1656
• Cytogenetics: 11q23.3
• Protein Pathways: RNA degradation
• Gene Summary: This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012]