COX5B (NM_001862) Human Untagged Clone

CAT#: SC126991

COX5B (untagged)-Human cytochrome c oxidase subunit Vb (COX5B), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COX5B
• Synonyms: COXVB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126991 sequence for NM_001862 edited (data generated by NextGen Sequencing)
  ATGGCTTCAAGGTTACTTCGCGGAGCTGGAACGCTGGCCGCGCAGGCCCTGAGGGCTCGC
  GGCCCCAGTGGCGCGGCCGCGATGCGCTCCATGGCATCTGGAGGTGGTGTTCCCACTGAT
  GAAGAGCAGGCGACTGGGTTGGAGAGGGAGATCATGCTGGCTGCAAAGAAGGGACTGGAC
  CCATACAATGTACTGGCCCCAAAGGGAGCTTCAGGCACCAGGGAAGACCCTAATTTAGTC
  CCCTCCATCTCCAACAAGAGAATAGTAGGCTGCATCTGTGAAGAGGACAATACCAGCGTC
  GTCTGGTTTTGGCTGCACAAAGGCGAGGCCCAGCGATGCCCCCGCTGTGGAGCCCATTAC
  AAGCTGGTGCCCCAGCAGCTGGCACACTGA
  
  Clone variation with respect to NM_001862.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001862 unedited
  NGGCGTCAGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAG
  CTTGTTCCCGGAAGTTTTGCTGCTAGTCGCGGACGCAATGGCTTCAAGGTTACTTCGCGG
  AGCTGGAACGCTGGCCGCGCAGGCCCTGAGGGCTCGCGGCCCCAGTGGCGCGGCCGCGAT
  GCGCTCCATGGCATCTGGAGGTGGTGTTCCCACTGTTGAAGAGCAGGCGACTGGGTTGGA
  GAGGGAGATCATGCTGGCTGCAAAGAAGGGACTGGACCCATACAATGTACTGGCCCCAAA
  GGGAGCTTCAGGCACCAGGGAAGACCCTAATTTAGTCCCCTCCATCTCCAACAAGAGAAT
  AGTANGCTGCATCTGTGAAGAGGACAATACCAGCGTCGTCTGGTTTTGGCTGCACANAGG
  CGAGGCCCAGCGATGCCCCCGCTGTGGAGCCCATTACAAAGCTGTGCCCCAGCAGCTGGC
  ACACTGAGCACCTGCACTAAATTACTCAAAAATGTGCTGTAAAGTTCCTTCTTTTCAGTA
  AAGACTAGCCATTGCATGGCCTCCTTCTCCCACTAAAAAAAAAAAAAAAACTCGACTCTA
  ATTTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGGGGCATCCTGGGGACCC
  TTTCCCAGGGCGTTTTCCTGGCCTTGGAATTGCCCCTTCCAGGGCCCCACAACCCTTGCC
  CCAATAAAAATTAATTGCCATAATTTGGCCTGACAAGGGGGCCCTCTTTAAAATTATGGG
  GGGAGGGGGTGGTATTGAAACAAGGCCAAATTTGAAAAACAACTTTTGGGGCGTGGGGGT
  TTTTGGGAAACAACGTGGGTGGAGGGGCACAATTTGGGCCAGTGAATCCCCCCCCCGGGG
  GAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001862
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001862.2, NP_001853.2
• RefSeq Size: 523 bp
• RefSeq ORF: 390 bp
• Locus ID: 1329
• UniProt ID: P10606
• Cytogenetics: 2q11.2
• Domains: COX5B
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008]