KIFAP3 (NM_014970) Human Untagged Clone

CAT#: SC126979

KIFAP3 (untagged)-Human kinesin-associated protein 3 (KIFAP3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KIFAP3
• Synonyms: dJ190I16.1; FLA3; KAP-1; KAP-3; KAP3; SMAP; Smg-GDS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC126979 representing NM_014970.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAAGGGGAGGACGCCAGATACCTCAAAAGGAAAGTTAAAGGAGGGAATATAGATGTACATCCATCA
  GAAAAAGCACTCATTGTTCACTATGAAGTGGAAGCTACCATTCTTGGAGAAATGGGGGACCCCATGTTG
  GGAGAACGAAAAGAATGTCAAAAAATCATTCGACTTAAGAGTCTCAATGCCAACACAGATATAACTTCC
  CTGGCAAGGAAGGTGGTTGAAGAATGTAAACTCATTCATCCTTCAAAACTAAATGAGGTAGAACAGCTG
  TTGTACTATCTACAGAACCGCCGTGATTCATTGTCAGGAAAAGAGAAAAAAGAAAAATCAAGCAAGCCT
  AAAGATCCACCTCCTTTTGAAGGAATGGAGATTGATGAAGTTGCTAACATTAATGACATGGATGAATAT
  ATTGAGTTATTATATGAAGATATTCCTGACAAAGTTCGGGGTTCTGCTTTGATCCTGCAGCTTGCTCGA
  AATCCTGATAACTTGGAAGAACTACTATTGAATGAAACTGCCCTTGGTGCATTAGCAAGGGTCCTGAGA
  GAAGACTGGAAGCAAAGTGTCGAGTTAGCTACAAACATAATTTACATCTTTTTTTGTTTCTCCAGCTTT
  TCTCAATTTCATGGACTTATTACTCACTATAAAATTGGAGCTCTGTGTATGAATATTATTGATCATGAG
  TTAAAAAGACATGAGCTTTGGCAAGAAGAACTCTCAAAGAAGAAGAAAGCTGTTGATGAAGACCCTGAA
  AACCAAACCTTGAGAAAGGATTATGAAAAAACCTTTAAAAAGTACCAGGGGCTTGTGGTAAAACAGGAA
  CAGCTATTACGAGTTGCTCTTTATTTGCTTCTGAATCTTGCTGAGGATACTCGTACCGAACTGAAAATG
  AGGAACAAGAACATAGTTCACATGTTGGTGAAAGCCCTTGATCGGGACAATTTTGAGCTGCTAATTTTA
  GTTGTGTCATTCTTGAAGAAACTCAGCATTTTTATGGAGAATAAAAATGATATGGTGGAAATGGATATT
  GTTGAAAAACTGGTGAAAATGATACCTTGTGAGCATGAAGACCTGCTGAATATCACCCTCCGACTTTTA
  CTAAACCTATCCTTTGACACAGGACTGAGGAATAAGATGGTACAAGTTGGACTGCTTCCCAAGCTCACT
  GCACTCCTAGGCAATGACAACTACAAACAAATAGCAATGTGTGTTCTTTACCACATAAGCATGGATGAC
  CGCTTTAAATCAATGTTTGCATACACTGACTGTATACCACAGTTAATGAAGATGCTGTTTGAATGTTCA
  GATGAACGAATTGACTTGGAACTCATTTCTTTCTGCATTAATCTTGCTGCTAACAAAAGAAATGTACAG
  CTTATCTGTGAAGGAAATGGGCTGAAGATGCTCATGAAGAGGGCTCTGAAGTTTAAGGATCCATTGCTG
  ATGAAAATGATTAGAAACATTTCTCAGCATGATGGACCAACTAAAAATCTGTTTATTGATTATGTTGGG
  GACCTTGCAGCCCAGATCTCTAATGATGAAGAAGAGGAGTTTGTGATTGAATGTTTGGGAACTCTTGCA
  AACTTGACCATTCCAGACTTAGACTGGGAATTGGTTCTTAAAGAATATAAGTTGGTTCCATACCTCAAG
  GATAAACTAAAACCAGGTGCTGCAGAAGATGATCTTGTTTTAGAAGTGGTTATAATGATTGGAACTGTA
  TCCATGGATGACTCTTGTGCTGCATTGCTAGCCAAATCTGGCATAATCCCTGCACTCATTGAATTGCTA
  AATGCTCAACAAGAAGATGATGAATTTGTGTGTCAGATAATTTATGTCTTCTACCAGATGGTTTTCCAC
  CAAGCCACAAGAGACGTCATAATCAAGGAAACACAGGCTCCAGCATATCTCATAGACCTAATGCATGAT
  AAGAATAATGAAATCCGAAAGGTCTGTGATAATACATTAGATATTATAGCGGAATATGATGAAGAATGG
  GCTAAGAAAATTCAGAGTGAAAAGTTTCGCTGGCATAACTCTCAGTGGCTGGAGATGGTAGAGAGTCGT
  CAGATGGATGAGAGTGAGCAGTACTTGTATGGTGATGATCGAATTGAGCCATACATTCATGAAGGAGAT
  ATTCTCGAAAGACCTGACCTTTTCTACAACTCAGATGGATTAATTGCCTCTGAAGGAGCCATAAGTCCC
  GATTTCTTCAATGATTACCACCTTCAAAATGGAGATGTTGTTGGGCAGCATTCATTTCCTGGCAGCCTT
  GGAATGGATGGCTTTGGCCAACCAGTTGGCATTCTTGGACGCCCTGCCACAGCATATGGATTCCGCCCT
  GATGAACCTTACTACTATGGCTATGGATCTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014970
• Insert Size: 2379 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014970.3
• RefSeq Size: 3011 bp
• RefSeq ORF: 2379 bp
• Locus ID: 22920
• UniProt ID: Q92845
• Cytogenetics: 1q24.2
• Domains: Armadillo_seg
• Protein Families: Druggable Genome
• MW: 91.2 kDa
• Gene Summary: The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (1) encodes the longest isoform (1).