FBXO22 (NM_147188) Human Untagged Clone

CAT#: SC126942

FBXO22 (untagged)-Human F-box protein 22 (FBXO22), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXO22
• Synonyms: FBX22; FISTC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126942 sequence for NM_147188 edited (data generated by NextGen Sequencing)
  ATGGAGCCGGTAGGCTGCTGCGGCGAGTGCCGCGGCTCCTCCGTAGACCCGCGGAGCACC
  TTCGTGTTGAGTAACCTGGCGGAGGTGGTGGAGCGTGTGCTCACCTTCCTGCCCGCCAAG
  GCGTTGCTGCGGGTGGCCTGCGTGTGCCGCTTATGGAGGGAGTGTGTGCGCAGAGTATTG
  CGGACCCATCGGAGCGTAACCTGGATCTCCGCAGGCCTGGCGGAGGCCGGCCACCTGGAG
  GGGCATTGCTTGGTTCGCGTGGTAGCAGAGGAGCTTGAGAATGTTCGCATCTTACCACAT
  ACAGTTCTTTACATGGCTGATTCAGAAACTTTCATTAGTCTGGAAGAGTGTCGTGGCCAT
  AAGAGAGCAAGGAAAAGAACTAGTATGGAAACAGCACTTGCCCTTGAGAAGCTATTCCCC
  AAACAATGCCAAGTCCTTGGGATTGTGACCCCAGGAATTGTAGTGACTCCAATGGGATCA
  GGTAGCAATCGACCTCAGGAAATAGAAATTGGAGAATCTGGTTTTGCTTTATTATTCCCT
  CAAATTGAAGGAATAAAAATACAACCCTTTCATTTTATTAAGGATCCAAAGAATTTAACA
  TTAGAAAGACATCAACTCACTGAAGTAGGTCTTTTAGATAACCCTGAACTTCGTGTGGTC
  CTTGTCTTTGGTTATAATTGCTGTAAGGTGGGAGCCAGTAATTATCTGCAGCAAGTAGTC
  AGCACTTTCAGTGATATGAATATCATCTTGGCTGGAGGCCAGGTGGACAACCTGTCATCA
  CTGACTTCTGAAAAGAACCCTCTGGATATTGATGCCTCGGGTGTGGTTGGACTGTCATTT
  AGTGGACACCGAATCCAGAGTGCCACTGTGCTCCTCAACGAGGACGTCAGTGATGAGAAG
  ACTGCTGAGGCTGCGATGCAGCGCCTCAAAGCGGCCAACATTCCAGAGCATAACACCATT
  GGCTTCATGTTTGCATGCGTTGGCAGGGGCTTTCAGTATTACAGAGCCAAGGGGAATGTT
  GAGGCTGATGCATTTAGAAAGTTTTTTCCTAGTGTTCCCTTATTCGGCTTCTTTGGAAAT
  GGAGAAATTGGATGTGATCGGATAGTCACTGGGAACTTTATATTGAGGAAATGTAATGAG
  GTAAAAGATGATGATCTGTTTCATAGCTATACAACAATAATGGCACTCATACATCTGGGG
  TCATCTAAATAA
  
  Clone variation with respect to NM_147188.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_147188 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGTTCCGGCGGGCTGGT
  GAGGAATGGAGCCGGTAGGCTGCTGCGGCGAGTGCCGCGGCTCCTCCGTAGACCCGCGGA
  GCACCTTCGTGTTGAGTAACCTGGCGGAGGTGGTGGAGCGTGTGCTCACCTTCCTGCCCG
  CCAAGGCGTTGCTGCGGGTGGCCTGCGTGTGCCGCTTATGGAGGGAGTGTGTGCGCAGAG
  TATTGCGGACCCATCGGAGCGTAACCTGGATCTCCGCAGGCCTGGCGGAGGCCGGCCACC
  TGGAGGGGCATTGCTTGGTTCGCGTGGTAGCAGAGGAGCTTGAGAATGTTCGCATCTTAC
  CACATACAGTTCTTTACATGGCTGATTCAGAAACTTTCATTAGTCTGGAAGAGTGTCGTG
  GCCATAAGAGAGCAAGGAAAAGAACTAGTATGGAAACAGCACTTGCCCTTGAGAAGCTAT
  TCCCCAAACAATGCCAAGTCCTTGGGATTGTGACCCCAGGAATTGTAGTGACTCCAATGG
  GATCAGGTAGCAATCGACCTCAGGAAATAGAAATTGGAGAATCTGGTTNTGCTTTATTAT
  TCCCTCAAATTGAAGGAATAAAAATACAACCCTTTCATTTTATTAAGGATCCAAAGAATT
  TAACATTAGAAAGACATCAACTCACTGAAGTAGGTCTTTTAGATACCCTGACTTCGNGTG
  GCCTTGTCTTTGTTATATTGCTGTAGGGTGGAGCCAGTATTATCTGCAGCAGTAGTCAGC
  ACTTTCATGATATGATATCATCTGGCCTGGAGCCAGGTGGACACCTGTATCACTGACTCT
  GAAAGAACCTCTGATATGAGCCCTGGNGGGGGGNTGGACTCCATTTATGGACACGATTCA
  GAGTGCCTGGCCTAAACAGGGACTAAGTAAAAAAAAATGGTGAGCTGGATCACGCTCAAC
  GCCACATCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_147188 unedited
  NTTCTGACTTGACCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTGAGTTC
  CACGCTATGTTTTATTGGTATAAAACCAAAATAAGATGATGTAATACTCTGGTCTACCAG
  TTAGTTAGCTATTTAGACCTAGGTGAAAAAAAAATATTTTTAAGGAAAGAAGAAAAATCT
  TTTACTTATAATAAAATACTGATTAGTCTTGTCAATTTATCTGACCAAAACAAAAATAGT
  TCTTTAATTTTTATCAAATAAGTAAAAAGTCAATAATAATATTGTTACTTCTGAAAAAAT
  TCATAAAACTGAAAGGCAGAGAAAAATTTCTCCAGCCATCAGTATATTTTTCTCCTTTAT
  TAAGGGAGAAAAAAGGTCTTCATGATTCTGCTAAAAGACATCACCTAACAACGCAAGGGG
  TAGTCAGGGAATGTCTTAAATCTATATCCTATTGATGAATACCCTTTTGCTCTTCCTATG
  CAGGAAGACTATTTAAGGTCATGTTACACAGCAGCTACATGATCTGGTGCAACACAACTA
  TAATCAGTTTGCTTCTGCCTGATGCAAAAGCTCTCAACTCCTAGTCAGTGTTATGTCCAA
  AGTTGTCCTTCATCTAATATATTAAATACTACCCTCATGTGATCTTAGAGCATCAATCAA
  AGCTACAAAAAAGATATATCTAAAGTTATTTTTGTTTGAAATACACATGGCCCAAGTTTC
  CATTTTCTGAAAAGGCAGAACCCAAAGTTACATATTATGAAAGCCACTTTAATTATTATT
  TAGATGACCCCAGATGTATGAGTGCCATTATTGTTGTATAGCTATGAAACAGATCATCAT
  CTTTTACCTCATTACATTTNCTCATATAAAGNTNCCAGTGACTATCCGATCACTNCATTT
  NCTNCTTTNCAAAGAAGCCCGATAGGGACACTNAGNAAAACT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_147188
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_147188.1, NP_671717.1
• RefSeq Size: 2818 bp
• RefSeq ORF: 1212 bp
• Locus ID: 26263
• UniProt ID: Q8NEZ5
• Cytogenetics: 15q24.2
• Domains: F-box
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and, as a transcriptional target of the tumor protein p53, is thought to be involved in degradation of specific proteins in response to p53 induction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.