MRE11 (NM_005590) Human Untagged Clone

CAT#: SC126932

MRE11A (untagged)-Human MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRE11
• Synonyms: ATLD; HNGS1; MRE11A; MRE11B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126932 sequence for NM_005590 edited (data generated by NextGen Sequencing)
  ATGAGTACTGCAGATGCACTTGATGATGAAAACACATTTAAAATATTAGTTGCAACAGAT
  ATTCATCTTGGATTTATGGAGAAAGATGCAGTCAGAGGAAATGATACGTTTGTAACACTC
  GATGAAATTTTAAGACTTGCCCAGGAAAATGAAGTGGATTTTATTTTGTTAGGTGGTGAT
  CTTTTTCATGAAAATAAGCCCTCAAGGAAAACATTACATACCTGCCTCGAGTTATTAAGA
  AAATATTGTATGGGTGATCGGCCTGTCCAGTTTGAAATTCTCAGTGATCAGTCAGTCAAC
  TTTGGTTTTAGTAAGTTTCCATGGGTGAACTATCAAGATGGCAACCTCAACATTTCAATT
  CCAGTGTTTAGTATTCATGGCAATCATGACGATCCCACAGGGGCAGATGCACTTTGTGCC
  TTGGACATTTTAAGTTGTGCTGGATTTGTAAATCACTTTGGACGTTCAATGTCTGTGGAG
  AAGATAGACATTAGTCCGGTTTTGCTTCAAAAAGGAAGCACAAAGATTGCGCTATATGGT
  TTAGGATCCATTCCAGATGAAAGGCTCTATCGAATGTTTGTCAATAAAAAAGTAACAATG
  TTGAGACCAAAGGAAGATGAGAACTCTTGGTTTAACTTATTTGTGATTCATCAGAACAGG
  AGTAAACATGGAAGTACTAACTTCATTCCAGAACAATTTTTGGATGACTTCATTGATCTT
  GTTATCTGGGGCCATGAACATGAGTGTAAAATAGCTCCAACCAAAAATGAACAACAGCTG
  TTTTATATCTCACAACCTGGAAGCTCAGTGGTTACTTCTCTTTCCCCAGGAGAAGCTGTA
  AAGAAACATGTTGGTTTGCTGCGTATTAAAGGGAGGAAGATGAATATGCATAAAATTCCT
  CTTCACACAGTGCGGCAGTTTTTCATGGAGGATATTGTTCTAGCTAATCATCCAGACATT
  TTTAACCCAGATAATCCTAAAGTAACCCAAGCCATACAAAGCTTCTGTTTGGAGAAGATT
  GAAGAAATGCTTGAAAATGCTGAACGGGAACGTCTGGGTAATTCTCACCAGCCAGAGAAG
  CCTCTTGTACGACTGCGAGTGGACTATAGTGGAGGTTTTGAACCTTTCAGTGTTCTTCGC
  TTTAGCCAGAAATTTGTGGATCGGGTAGCTAATCCAAAAGACATTATCCATTTTTTCAGG
  CATAGAGAACAAAAGGAAAAAACAGGAGAAGAGATCAACTTTGGGAAACTTATCACAAAG
  CCTTCAGAAGGAACAACTTTAAGGGTAGAAGATCTTGTAAAACAGTACTTTCAAACCGCA
  GAGAAGAATGTGCAGCTCTCACTGCTAACAGAAAGAGGGATGGGTGAAGCAGTACAAGAA
  TTTGTGGACAAGGAGGAGAAAGATGCCATTGAGGAATTAGTGAAATACCAGTTGGAAAAA
  ACACAGCGATTTCTTAAAGAACGTCATATTGATGCCCTCGAAGACAAAATCGATGAGGAG
  GTACGTCGTTTCAGAGAAACCAGACAAAAAAATACTAATGAAGAAGATGATGAAGTCCGT
  GAGGCTATGACCAGGGCCAGAGCACTCAGATCTCAGTCAGAGGAGTCTGCTTCTGCCTTT
  AGTGCTGATGACCTTATGAGTATAGATTTAGCAGAACAGATGGCTAATGACTCTGATGAT
  AGCATCTCAGCAGCAACCAACAAAGGAAGAGGCCGAGGAAGAGGTCGAAGAGGTGGAAGA
  GGGCAGAATTCAGCATCGAGAGGAGGGTCTCAANNNNNNNNNNNCTTTAAATCTACAAGA
  CAGCAGCCTTCCCGAAATGTCACTACTAAGAATTATTCAGAGGTGATTGAGGTAGATGAA
  TCAGATGTGGAAGAAGACATTTTTCCTACCACTTCAAAGACAGATCAAAGGTGGTCCAGC
  ACATCATCCAGCAAAATCATGTCCCAGAGTCAAGTATCGAAAGGGGTTGATTTTGAATCA
  AGTGAGGATGATGATGATGATCCTTTTATGAACACTAGTTCTTTAAGAAGAAATAGAAGA
  TAA
  
  Clone variation with respect to NM_005590.3
  1774 a=>n;1775 g=>n;1776 a=>n;1777 g=>n;1778 g=>n;1779 a=>n;1780 a=>n;1781 g=>n;1782 a=>n;1783 g=>n;1784 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005590 unedited
  CTGACATTTGAGTACAGTACCCAGGGGTTCTTGGAGAAGAACCTGGTCCCAGAGGAGCTT
  GACTGACCATAAAAATGAGTACTGCAGATGCACTTGATGATGAAAACACATTTAAAATAT
  TAGTTGCAACAGATATTCATCTTGGATTTATGGAGAAAGATGCAGTCAGAGGAAATGATA
  CGTTTGTAACACTCGATGAAATTTTAAGACTTGCCCAGGAAAATGAAGTGGATTTTATTT
  TGTTAGGTGGTGATCTTTTTCATGAAAATAAGCCCTCAAGGAAAACATTACATACCTGCC
  TCGAGTTATTAAGAAAATATTGTATGGGTGATCGGCCTGTCCAGTTTGAAATTCTCAGTG
  ATCAGTCAGTCAACTTTGGTTTTAGTAAGTTTCCATGGGTGAACTATCAAGATGGCAACC
  TCAACATTTCAATTCCAGTGTTTAGTATTCATGGNCATCATGACGATCCCACAGGGATGC
  ACTTTGTGCCTTGGACATTNTAAGTTGTGCTGGATTTGTNAATCACTTTGGACGTTCAAT
  GTCTGTGGAGAGATAGACATTAGTCCGGTTTTGCTTAAAAAGGAGCACAAGATTGCCTAT
  ATGGTTTAGGATCCATTCCGATGAAAGGCTCTATCGATGTTTGTCATAAAAAGTACATGT
  TGAGACAAGGAGAGAGACTCTGGGGTACTTATTGTGTTCTCAGACAGAGTACTTGGAGCC
  TACCTCTTCAACATTTTGGAGCTCTTGATCTTGAATTGGGCCTGACTGATGAAAAACTCA
  CAAAGACACCCTGTTTTTCTCAACTGAAGCAGGGTCTCTCTCCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005590 unedited
  TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTAAGGAAAAAAAC
  TTTATTCCTTTTTTCCTAGGAACTTGTCAGGATACTTTAGTGACCATTCCCTCACTATAA
  CTCTCACCCAGACCCACCTAACTGATGCATGAGAAGAGCCACTAGCTGATGTGGCCCACA
  GCTCAGACTGAGCCAGCATGGCTTGCGCAGGGGTAGGTAGCACAGCATCATCCAGACACT
  GCCTCCCCTGGTAGCTTCCATTACATCATGGACAGCACTGCTCTTCCCTGACTTAACCTT
  CTGCTTTGTTCAAATACTAGAAATATTAGATTTCAAGTTCAATAAAGATGTGGGCAGATC
  TGCCTAAGAATATTTCACAGGACAACGCCTTCCACTGAGTTAATGCTACTTGCTCAAATA
  ACCCTTATGCTCAAGCCAGGTACAGAAAAAATATTTTTAAGAAAGTCAATACAATGTTAT
  ATTGCAAGTTCTGACTTACGCCTCTTCAAACGTGCTATATGAGGCAGCCACTAACCAAGT
  GTCTGTCTCTTATAATTNGACACATTCCAGGATGACTATTCTTAATGGTCATGAGTATCA
  CTGAGTCAAGGCTCTGCCTCCTACCTATAAAGAAATGTCGGGCCCACTGTACCTCCGAAG
  ACGTATAACTCCCCACTTATAAGTATCGCTGGTCGCCCACCACCCCCAGAAGGAATAACC
  CTCAGGGCCTCTTTTCCCCGAGGGATCATATCGAATGGGGTTATTTAACCTTACCTCTGA
  AGCAGGCCCATTCCTCATCTCTCCCCGCAAACACGGTTGGACTGCACCTTTTACATGGGC
  CTTTACGAATTCTCGACCTCGCCCTCTCCGAATGTCTCTCGGGCACCCCCAGGGAATCAT
  ACCTGCTCACGCGCAACCTTGCTTTGCTTTCGCCTCCCCCTCCCTATCTCTCTCCAGGGG
  TCACGCCCCCCCTCTTAATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005590
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005590.3, NP_005581.2
• RefSeq Size: 5164 bp
• RefSeq ORF: 2043 bp
• Locus ID: 4361
• UniProt ID: P49959
• Cytogenetics: 11q21
• Domains: Metallophos, Mre11_DNA_bind
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Homologous recombination, Non-homologous end-joining
• Gene Summary: This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform 2 lacks an internal segment, compared to isoform 1.