SHC (SHC1) (NM_003029) Human Untagged Clone

CAT#: SC126613

SHC1 (untagged)-Human SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SHC
• Synonyms: SHC; SHCA
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003029 edited
  GGGGAGAAAGTTGGAGCGGTAACCTAAGCTGGCAGTGGCGTGATCCGGCACCAAATCGGC
  CCGCGGTGCGGTGCGGAGACTCCATGAGGCCCTGGACATGAACAAGCTGAGTGGAGGCGG
  CGGGCGCAGGACTCGGGTGGAAGGGGGCCAGCTTGGGGGCGAGGAGTGGACCCGCCACGG
  GAGCTTTGTCAATAAGCCCACGCGGGGCTGGCTGCATCCCAACGACAAAGTCATGGGACC
  CGGGGTTTCCTACTTGGTTCGGTACATGGGTTGTGTGGAGGTCCTCCAGTCAATGCGTGC
  CCTGGACTTCAACACCCGGACTCAGGTCACCAGGGAGGCCATCAGTCTGGTGTGTGAGGC
  TGTGCCGGGTGCTAAGGGGGCGACAAGGAGGAGAAAGCCCTGTAGCCGCCCGCTCAGCTC
  TATCCTGGGGAGGAGTAACCTGAAATTTGCTGGAATGCCAATCACTCTCACCGTCTCCAC
  CAGCAGCCTCAACCTCATGGCCGCAGACTGCAAACAGATCATCGCCAACCACCACATGCA
  ATCTATCTCATTTGCATCCGGCGGGGATCCGGACACAGCCGAGTATGTCGCCTATGTTGC
  CAAAGACCCTGTGAATCAGAGAGCCTGCCACATTCTGGAGTGTCCCGAAGGGCTTGCCCA
  GGATGTCATCAGCACCATTGGCCAGGCCTTCGAGTTGCGCTTCAAACAATACCTCAGGAA
  CCCACCCAAACTGGTCACCCCTCATGACAGGATGGCTGGCTTTGATGGCTCAGCATGGGA
  TGAGGAGGAGGAAGAGCCACCTGACCATCAGTACTATAATGACTTCCCGGGGAAGGAACC
  CCCCTTGGGGGGGGTGGTAGACATGAGGCTTCGGGAAGGAGCCGCTCCAGGGGCTGCTCG
  ACCCACTGCACCCAATGCCCAGACCCCCAGCCACTTGGGAGCTACATTGCCTGTAGGACA
  GCCTGTTGGGGGAGATCCAGAAGTCCGCAAACAGATGCCACCTCCACCACCCTGTCCAGG
  CAGAGAGCTTTTTGATGATCCCTCCTATGTCAACGTCCAGAACCTAGACAAGGCCCGGCA
  AGCAGTGGGTGGTGCTGGGCCCCCCAATCCTGCTATCAATGGCAGTGCACCCCGGGACCT
  GTTTGACATGAAGCCCTTCGAAGATGCTCTTCGCGTGCCTCCACCTCCCCAGTCGGTGTC
  CATGGCTGAGCAGCTCCGAGGGGAGCCCTGGTTCCATGGGAAGCTGAGCCGGCGGGAGGC
  TGAGGCACTGCTGCAGCTCAATGGGGACTTCCTGGTACGGGAGAGCACGACCACACCTGG
  CCAGTATGTGCTCACTGGCTTGCAGAGTGGGCAGCCTAAGCATTTGCTACTGGTGGACCC
  TGAGGGTGTGGTTCGGACTAAGGATCACCGCTTTGAAAGTGTCAGTCACCTTATCAGCTA
  CCACATGGACAATCACTTGCCCATCATCTCTGCGGGCAGCGAACTGTGTCTACAGCAACC
  TGTGGAGCGGAAACTGTGATCTGCCCTAGCGCTCTCTTCCAGAAGATGCCCTCCAATCCT
  TTCCACCCTATTCCCTAACTCTCGGGACCTCGTTTGGGAGTGTTCTGTGGGCTTGGCCTT
  GTGTCAGAGCTGGGAGTAGCATGGACTCTGGGTTTCATATCCAGCTGAGTGAGAGGGTTT
  GAGTCAAAAGCCTGGGTGAGAATCCTGCCTCTCCCCAAACATTAATCACCAAAGTATTAA
  TGTACAGAGTGGCCCCTCACCTGGGCCTTTCCTGTGCCAACCTGATGCCCCTTCCCCAAG
  AAGGTGAGTGCTTGTCATGGAAAATGTCCTGTGGTGACAGGCCCAGTGGAACAGTCACCC
  TTCTGGGCAAGGGGGAACAAATCACACCTCTGGGCTTCAGGGTATCCCAGACCCCTCTCA
  ACACCCGCCCCCCCCATGTTTAAACTTTGTGCCTTTGACCATCTCTTAGGTCTAATGATA
  TTTTATGCAAACAGTTCTTGGACCCCTGAATTCAATGACAGGGATGCCAACACCTTCTTG
  GCTTCTGGGACCTGTGTTCTTGCTGAGCACCCTCTCCGGTTTGGGTTGGGATAACAGAGG
  CAGGAGTGGCAGCTGTCCCCTCTCCCTGGGGATATGCAACCCTTAGAGATTGCCCCAGAG
  CCCCACTCCCGGCCAGGCGGGAGATGGACCCCTCCCTTGCTCAGTGCCTCCTGGCCGGGG
  CCCCTCACCCCAAGGGGTCTGTATATACATTTCATAAGGCCTGCCCTCCCATGTTGCATG
  CCTATGTACTCTACGCCAAAGTGCAGCCCTTCCTCCTGAAGCCTCTGCCCTGCCTCCCTT
  TCTGGGAGGGCGGGGTGGGGGTGACTGAATTTGGGCCTCTTGTACAGTTAACTCTCCCAG
  GTGGATTTTGTGGAGGTGAGAAAAGGGGCATTGAGACTATAAAGCAGTAGACAATCCCCC
  ACATACCATCTGTAGAGTTGGAACTGCATTCTTTTAAAGTTTTATATGCATATATTTTAG
  GGCTGTAGACTTACTTTCCTATTTTCTTTTCCATTGCTTATTCTTGAGCACAAAATGATA
  ATCAATTATTACATTTATACATCACCTTTTTGACTTTTCCAAGCCCTTTTACAGCTCTTG
  GCATTTTCCTCGCCTAGGCCTGTGAGGTAACTGGGATCGCACCTTTTATACCAGAGACCT
  GAGGCAGATGAAATTTATTTCCATCTAGGACTAGAAAAACTTGGGTCTCTTACCGCGAGA
  CTGAGAGGCAGAAGTCAGCCCGAATGCCTGTCAGTTTCATGGAGGGGAAACGCAAAACCT
  GCAGTTCCTGAGTACCTTCTACAGGCCCGGCCCAGCCTAGGCCCGGGGTGGCCACACCAC
  AGCAAGCCGGCCCCCCCTCTTTTGGCCTTGTGGATAAGGGAGAGTTGACCGTTTTCATCC
  TGGCCTCCTTTTGCTGTTTGGATGTTTCCACGGGTCTCACTTATACCAAAGGGAAAACTC
  TTCATTAAAGTCCGTATTTCTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003029 unedited
  NNNCCATATAACACCCGCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCT
  ATATAAGCAGAGCTCATTTAGGTGACACTATGAGAATACACAGCTTACGTTGTTCTTTTT
  TGCAGNNCGGCCTGCGAATTCTGGCACTGAGGGCTGGGGAGAAAGTTGGAGCGGTAACCT
  AAGCTGGCAGTGGCGTGATCCGGCACCAAATCGGCCCGCGGTGCGGTGCGGAGACTCCAT
  GAGGCCCTGGACATGAACAAGCTGAGTGGAGGCGGCGGGCGCAGGACTCGGGTGGAAGGG
  GGCCAGCTTGGGGGCGAGGAGTGGACCCGCCACGGGAGCTTTGTCAATAAGCCCACGCGG
  GGCTGGCTGCATCCCAACGACAAAGTCATGGGACCCGGGGTTTCCTACTTGGTTCGGTAC
  ATGGGTTGTGTGGAGGTCCTCCAGTCAATGCGTGCCCTGGACTTCAACACCCGGACTCAG
  GTCACCAGGGAGGCCATCAGTCTGGTGTGTGAGGCTGTGCCGGGTGCTAAGGGGGCGACA
  AGGAGGAGAAAGCCCTGTAGCCGCCCGCTCAGCTCTATCCTGGGGAGGAGTAACCTGAAA
  TTTGCTGGAATGCCAATCACTCTCACCGTCTCCACCAGCAGCCTCAACCTCATGGCCGCA
  GACTGCAAACAGATCATCGCCAACCACCACATGCAATCTATCTCATTTGCATCCTGGCGG
  NGATCCGGACACAGCCTGAGTATGTCGCCTATGTTGCCAAAGACCCTGTGAATCAGAGAG
  CCTGCCACATTCTGGAGTGTCCCTGAAGGGCTTGCTCCAGGATGTCATCAGCACCATTGG
  CCAGGCCTTTCGAGTTGCGCTTCAACAGTACCTCAGGAACCACCCAGACTGGTACTCTCA
  TCACGNAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003029 unedited
  NGGGAATTACTATGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTT
  TAAAAAAAAACGGACTTTAATGAAGAGTTTTCCCTTTGGTATAAGTGAGACCCGTGGAAA
  CATCCAAACAGCAAAAGGAGGCCAGGATGAAAACGGTCAACTCTCCCTTATCCACAAGGC
  CAAAAGAGGGGGGGCCGGCTTGCTGTGGTGTGGCCACCCCGGGCCTAGGCTGGGCCGGGC
  CTGTAGAAGGTACTCAGGAACTGCAGGTTTTGCGTTTCCCCTCCATGAAACTGACAGGCA
  TTCGGGCTGACTTCTGCCTCTCAGTCTCGCGGTAAGAGACCCAAGTTTTTCTAGTCCTAG
  ATGGAAATAAATTTCATCTGCCTCAGGTCTCTGGTATAAAAGGTGCGATCCCAGTTACCT
  CACAGGCCTAGGCGAGGAAAATGCCAAGAGCTGTAAAAGGGCTTGGAAAAGTCAAAAAGG
  TGATGTATAAATGTAATAATTGATTATCATTTTGTGCTCAAGAATAAGCAATGGAAAAGA
  AAATAGGAAAGTAAGTCTACAGCCCTAAAATATATGCATATAAAACTTTAAAAGAATGCA
  GTTCCAACTCTACAGATGGTATGTGGGGGATTGTCTACTGCTTTATAGTCTCAATGCCCC
  CTTTCTCACCTCCACAAAATCCACCTGGGGAGAGTTAACTGTACAAGAAGCCCAAATTCA
  GTCACCCCCACCCCGCCCTCCCAAAAAGGGAGGCAGGGCAAAGGCCTTCAGGAGAAAAGG
  CTGCACCTTTGGCGTAAAGTACATAGGCATGCACCATGGGAGGGCAGCCCCTATGAAAAG
  TATTACCGACCCCTGGGGTGAGGGGCCCGGCCAGGAGCACTGAACAAGGAGGGGTCCATC
  TCCGCCTGGCCGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003029
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain 3bp deletion compared with NM_003029.3.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003029.3, NP_003020.2
• RefSeq Size: 3076 bp
• RefSeq ORF: 1425 bp
• Locus ID: 6464
• UniProt ID: P29353
• Cytogenetics: 1q21.3
• Domains: SH2, PID
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Chemokine signaling pathway, Chronic myeloid leukemia, Dilated cardiomyopathy, ErbB signaling pathway, Focal adhesion, Glioma, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis
• Gene Summary: This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (2) has an alternate 5' sequence, as compared to variant 3. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 3.