PAK4 (NM_001014832) Human Untagged Clone

CAT#: SC126462

PAK4 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAK4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001014832 edited
  TTCAACATGGCGGCGGGAGTGTCCGCGGTGGTGGCGGTGCAAGAGAGCTGAAGGAGGCGC
  GAGGGCGCGGAGTTCCAGGCCGAGCAGTTAGGCCGCGAGCGACTGCGGCGCCGAGCCGGC
  CGCACCGAGTCCCCGGCACCATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATCTCCGCGC
  CGTCCAACTTCGAGCACCGCGTGCACACGGGCTTCGACCAGCACGAGCAGAAGTTCACGG
  GGCTGCCCCGCCAGTGGCAGAGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAGCCCCTCG
  TCGACCCCGCCTGCATCACCTCCATCCAGCCCGGGGCCCCCAAGACCATCGTGCGGGGCA
  GCAAAGGTGCCAAAGATGGGGCCCTCACGCTGCTGCTGGACGAGTTTGAGAACATGTCGG
  TGACACGCTCCAACTCCCTGCGGAGAGACAGCCCGCCGCCGCCCGCCCGTGCCCGCCAGG
  AAAATGGGATGCCAGAGGAGCCGGCCACCACGGCCAGAGGGGGCCCAGGGAAGGCAGGCA
  GCCGAGGCCGGTTCGCCGGTCACAGCGAGGCGGGTGGCGGCAGTGGTGACAGGCGACGGG
  CGGGGCCAGAGAAGAGGCCCAAGTCTTCCAGGGAGGGCTCAGGGGGTCCCCAGGAGTCCT
  CCCGGGACAAACGCCCCCTCTCCGGGCCTGATGTCGGCACCCCCCAGCCTGCTGGTCTGG
  CCAGTGGGGCGAAACTGGCAGCTGGCCGGCCCTTTAACACCTACCCGAGGGCTGACACGG
  ACCACCCATCCCGGGGTGCCCAGGGGGAGCCTCATGACGTGGCCCCTAACGGGCCATCAG
  CGGGGGGCCTGGCCATCCCCCAGTCCTCCTCCTCCTCCTCCCGGCCTCCCACCCGAGCCC
  GAGGTGCCCCCAGCCCTGGAGTGCTGGGACCCCACGCCTCAGAGCCCCAGCTGGCCCCTC
  CAGCCTGCACCCCCGCCGCCCCTGCTGTTCCTGGGCCCCCTGGCCCCCGCTCACCACAGC
  GGGAGCCACAGCGAGTATCCCATGAGCAGTTCCGGGCTGCCCTGCAGCTGGTGGTGGACC
  CAGGCGACCCCCGCTCCTACCTGGACAACTTCATCAAGATTGGCGAGGGCTCCACGGGCA
  TCGTGTGCATCGCCACCGTGCGCAGCTCGGGCAAGCTGGTGGCCGTCAAGAAGATGGACC
  TGCGCAAGCAGCAGAGGCGCGAGCTGCTCTTCAACGAGGTGGTAATCATGAGGGACTACC
  AGCACGAGAATGTGGTGGAGATGTACAACAGCTACCTGGTGGGGGACGAGCTCTGGGTGG
  TCATGGAGTTCCTGGAAGGAGGCGCCCTCACCGACATCGTCACCCACACCAGGATGAACG
  AGGAGCAGATCGCGGCCGTGTGCCTTGCAGTGCTGCAGGCCCTGTCGGTGCTCCACGCCC
  AGGGCGTCATCCACCGGGACATCAAGAGCGACTCGATCCTGCTGACCCATGATGGCAGGG
  TGAAGCTGTCAGACTTTGGGTTCTGCGCCCAGGTGAGCAAGGAAGTGCCCCGAAGGAAGT
  CGCTGGTCGGCACGCCCTACTGGATGGCCCCAGAGCTCATCTCCCGCCTTCCCTACGGGC
  CAGAGGTAGACATCTGGTCGCTGGGGATAATGGTGATTGAGATGGTGGACGGAGAGCCCC
  CCTACTTCAACGAGCCACCCCTCAAAGCCATGAAGATGATTCGGGACAACCTGCCACCCC
  GACTGAAGAACCTGCACAAGGTGTCGCCATCCCTGAAGGGCTTCCTGGACCGCCTGCTGG
  TGCGAGACCCTGCCCAGCGGGCCACGGCAGCCGAGCTGCTGAAGCACCCATTCCTGGCCA
  AGGCAGGGCCGCCTGCCAGCATCGTGCCCCTCATGCGCCAGAACCGCACCAGATGAGGCC
  CAGCGCCCTTCCCCTCAACCAAAGAGCCCCCCGGGTCACCCCCGCCCCACTGAGGCCAGT
  AGGGGGCCAGGCCTCCCACTCCTCCCAGCCCGGGAGATGCTCCGCGTGGCACCACCCTCC
  TTGCTGGGGGTAGATGAGACCCTACTACTGAACTCCAGTTTTGATCTCGTGACTTTTAGA
  AAAACACAGGGACTCGTGGGAGCAAGCGAGGCTCCCAGGACCCCCACCCTCTGGGACAGG
  CCCTCCCCCATGTTCTTCTGTCTCCAGGAAGGGCAGCAGCCCTCCCATCACTGGAAGTCT
  GCAGTGGGGGTCGCTGGGGGTGGAGAGAACACTAAGAGGTGAACATGTATGAGTGTGTGC
  ACGCGTGTGAGTGTGCATGTGTGTGTGTGTGCAAAGGTCCAGCCACCCCGTCCTCCAGCC
  TGCAAGGGGTGTCTGGCGCCTTGCCTGACACCCAGCCCCCTCTCCCCCTGAGCCATTGTG
  GGGGTCGATCATGAATGTCCGAAGAGTGGCCTTTTCCCGTAGCCCTGCGCCCCCTTTCTG
  TGGCTGGATGGGGAGACAGGTCAGGGCCCCCCACCCTCTCCAGCCCCTGCAGCAAATGAC
  TACTGCACCTGGACAGCCTCCTCTTTTCTAGAAGTCTATTTATATTGTCATTTTATAACA
  CTCTAGCCCCTGCCCTTATTGGGGGACAGATGGTCCCTGTCCTGCGGGGTGGCCCTGGCA
  GAACCACTGCCTGAAGAACCAGGTTCCTGCCCGGTCAGCGCAGCCCCAGCCCGCCCACCC
  CTGCCTCGAGTTAGTTTTACAATTAAAACATTGTCTTGTTTAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001014832 unedited
  NGGGCTAGATTTGTATACCATTTTATGGGCGGCCGCGNATTCACATCTGGTACCGGTCCG
  GAATTCCCGGGATATCGTCGACCCACGCGTCCGTTCAACATGGCGGCGGGAGTGTCCGCG
  GTGGTGGCGGTGCAAGAGAGCTGAAGGAGGCGCGAGGGCGCGGAGTTCCAGGCCGAGCAG
  TTAGGCCGCGAGCGACTGCGGCGCCGAGCCGGCCGCACCGAGTCCCCGGCACCATGTTTG
  GGAAGAGGAAGAAGCGGGTGGAGATCTCCGCGCCGTCCAACTTCGAGCACCGCGTGCACA
  CGGGCTTCGACCAGCACGAGCAGAAGTTCACGGGGCTGCCCCGCCAGTGGCAGAGCCTGA
  TCGAGGAGTCGGCTCGCCGGCCCAAGCCCCTCGTCGACCCCGCCTGCATCACCTCCATCC
  AGCCCGGGGCCCCCAAGACCATCGTGCGGGGCAGCAAAGGTGCCAAAGATGGGGCCCTCA
  CGCTGCTGCTGGACGAGTTTGAGAACATGTCGGTGACACGCTCCAACTCCCTGCGGAAAG
  ACAGCCCGCCGCCGCCCGCCCGTGCCCGCCAGGAAAATGGGATGCCAGAGGAGCCGGCCA
  CCACGGCCAGAGGGGGCCCAGGGAAGGCAGGCAGCCGAGGCCGGTTCGCCGGTCACAGCG
  AGGCGGGTGGCGGCAGTGGTGACAGGCGACGGGCGGGGCCAGAAAAGAGGCCCAAGTCTT
  CCAGGGAGGGCTCAGGGGGTCCCCAGGAGTCCTCCCGGGACAACGCCCCTCTCCGGGCCT
  GATGTCGGCACCCCCCAGCCTGCTGGTCTGGCCATGGGGCGAAACTGCCAGCTGGGCCGC
  CCTTTACACCTACCCGAGGGCTGAACGGACAACCATCCCGGGTGCCCAGGGGAACCTA
  
• Restriction Sites: Please inquire
• ACCN: NM_001014832
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014832.1, NP_001014832.1
• RefSeq Size: 2765 bp
• RefSeq ORF: 1776 bp
• Locus ID: 10298
• UniProt ID: O96013
• Cytogenetics: 19q13.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
• Gene Summary: PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) lacks an exon in the 5' UTR, and encodes the same isoform (1), as compared to variant 1.