Ribosomal protein S10 (RPS10) (BC030568) Human Untagged Clone

CAT#: SC125872

(untagged)-Human cDNA clone MGC:45392 IMAGE:5526694, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS10
• Synonyms: RPS10_2_147
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC030568 edited
  AGTAAAGGTTTGAAAAAGGAAAGTTTTATTAGAAAGAAAGAACGTGCCTTTCCAGCCCCC
  CACCCGGACCCTACAGCCACCGAGATGTTGATGCCTAAGAAGAACTGGATTGCCATTTAT
  GAACTCCTTTTTAAGGAGGGAGTCATGGTGGCCAAGAAGGATGTCCACATGCCTAAGCAC
  CCGGAGCTGGCAGACAAGAATGCGCCCAACCTTCGTGTCATGAAGGCCATGCAGTCTCTC
  AAGTCCCGAGGCTACGTGAAGGAAGTTTGCCTGGAGACATTTCTACTGGTACCTTACCAA
  TGAGGGTATCCAGTATCTCTGTGATTACCTTCATCTGCCCTCGGAGATTGTGCCTGCCAC
  CTTACACCGCAGCTGTCCAGAGACTGGCAGGCCTTGGCCTAAAGTCTGGAGGGTGAGCGA
  CCTGCAAGACTCACAAGAGGGGAAGCCAACAGAGATACCTACAAATGGAATGCTGTGCCC
  CCTGGTGCCAACAAGAAAGCCGAGGCTGGGGCCGGGTCAGCAACCAAATTCCAGTTCAGA
  GATGGATTTGGTTGTGGACGTGGTCAGCCACCTCAGTAAAATTGGAGAGGATTATTATGC
  AATGAATAAACTTACAGTCAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC030568 unedited
  NNNGGGTTCAGAATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATCCCGGGATATC
  GTCGACCCACGCGTCCGCTATACTGTTGTCAGTAAAGGTTTGAAAAAGGAAAGTTTTATT
  AGAAAGAAAGAACGTGCCTTTCCAGCCCCCCACCCGGACCCTACAGCCACCGAGATGTTG
  ATGCCTAAGAAGAACTGGATTGCCATTTATGAACTCCTTTTTAAGGAGGGAGTCATGGTG
  GCCAAGAAGGATGTCCACATGCCTAAGCACCCGGAGCTGGCAGACAAGAATGCGCCCAAC
  CTTCGTGTCATGAAGGCCATGCAGTCTCTCAAGTCCCGAGGCTACGTGAAGGAAGTTTGC
  CTGGAGACATTTCTACTGGTACCTTACCAATGAGGGTATCCAGTATCTCTGTGATTACCT
  TCATCTGCCCTCGGAGATTGTGCCTGCCACCTTACACCGCAGCTGTCCAGAGACTGGCAG
  GCCTTGGCCTAAAGTCTGGAGGGTGAGCGACCTGCAAGACTCACAAGAGGGGAAGCCAAC
  AGAGATACCTACAAATGGAATGCTGTGCCCCCTGGTGCCAACAAGAAAGCCGAGGCTGGG
  GCCGGGTCAGCAACCAAATTCCAGTTCAGAGATGGATTTGGTTGTGGACGTGGTCAGCCA
  CCTCAGTAAAATTGGAGAGGATTATTATGCAATGAATAAACTTACAGTCAAAAAAAAAAA
  AAAAAGGGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCCGGTGGCATCCCTGTGA
  CCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGT
  CCTAATAAAATAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATGGTGAGG
  GGGGNNGGGGGGGGGGGTTGNNANNNCCA
  
• Restriction Sites: NotI-NotI
• ACCN: BC030568
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030568.2
• RefSeq Size: 635 bp
• Locus ID: 6204
• Cytogenetics: 6p21.31
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternate splicing results in multiple transcript variants that encode the same protein. Naturally occurring read-through transcription occurs between this locus and the neighboring locus NUDT3 (nudix (nucleoside diphosphate linked moiety X)-type motif 3).[provided by RefSeq, Feb 2011]