PHF7 (NM_016483) Human Untagged Clone

CAT#: SC125634

PHF7 (untagged)-Human PHD finger protein 7 (PHF7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHF7
• Synonyms: HSPC045; HSPC226; NYD-SP6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_016483 edited
  GTCGAGAGCGGAGTCTTCGCCGGCGCAGTCACCGCGCAGGGCGGCCGCCCGGAGGTAGCT
  ACCACGGCCTGTGTCAACGACTAAAGCTCCAGTACAGCGGCGCCCTCAGACAGCTGGGAG
  GGTGGCTCTGGCCGGGAGCGGCGGCCGGTGAGCTACCGCGAGGAGGAGCGGCGGAGGCGA
  CCTCGGCCCGGCCCTGCACTGGCCGCCCGGCAGGCGCGACATGAGCCTGGTCTGGCATCC
  GCGGGATGCTCCTTAAGCCCCTTCTCCGGCTGTTAACCTCCGGGGAACGGTTGTGACCAC
  ACCGACACGTATTTTACAGATAAATCATTCTTGCGGCGGCGGGTCGAACACGTTTATTTA
  TTTTTTATTTTCTCAACAAGCTTTTACCCAGCACCTGTCCAGTGAAACAACTTGATAATC
  GTTTCGAGGGGCGTCCGCCGGGTTAGGAAGCCACTGCCTGGCAGCTTGTGGAAGCCTCAT
  TTGCAAAGCCACCCCTCAGATGTTTTGAAGATCGTGACGTCTTGTAACTAGCAGTGTGTG
  CACAGAATCCTACTCAAGGAACGTCTTGGCCCAGCGATGCAAAGAACTGAAGTTTCAAGC
  TGGAAGAGCCTGTATTGTCCTCACAATAGTATAGAAGAATTCAAGAGAGGAGAGAGAGAC
  AGCACCGAATGAAGACTGTAAAAGAAAAGAAGGAATGCCAGAGATTGAGAAAATCTGCCA
  AGACTAGGAGGGTAACCCAGAGGAAACCGTCTTCAGGGCCTGTTTGCTGGCTATGCCTTC
  GAGAACCTGGGGATCCCGAAAAATTAGGGGAATTTCTTCAGAAAGACAATATCAGCGTGC
  ATTATTTCTGTCTTATCTTATCTAGTAAGCTGCCTCAGAGGGGCCAGTCCAACAGAGGTT
  TCCATGGATTTCTGCCTGAAGACATCAAAAAGGAGGCAGCCCGGGCTTCTAGGAAGATCT
  GCTTTGTGTGCAAGAAAAAGGGAGCTGCTATCAACTGCCAGAAGGATCAGTGCCTCAGAA
  ACTTCCATCTGCCTTGTGGCCAAGAAAGGGGTTGCCTTTCACAATTTTTTGGAGAGTACA
  AATCATTTTGTGACAAACATCGCCCAACACAGAACATCCAACATGGGCATGTGGGGGAGG
  AAAGCTGCATCTTATGTTGTGAAGACTTATCCCAACAGAGTGTTGAGAACATCCAGAGCC
  CGTGTTGTAGTCAAGCCATCTACCACCGCAAGTGCATACAGAAATATGCCCACACATCAG
  CAAAGCATTTCTTCAAATGTCCACAGTGTAACAATCGAAAAGAGTTTCCTCAAGAAATGC
  TGAGAATGGGAATTCATATTCCAGACAGAGATGCTGCCTGGGAACTCGAGCCAGGGGCTT
  TCTCAGACTTATATCAGCGCTATCAGCACTGTGATGCCCCCATCTGTCTGTATGAACAAG
  GCAGAGACAGCTTTGAGGATGAAGGGAGGTGGTGCCTCATTCTGTGTGCTACATGCGGAT
  CCCACGGAACCCACAGGGACTGCTCCTCTCTTAGATCTAACAGTAAGAAATGGGAGTGTG
  AGGAGTGTTCACCTGCTGCAGCCACAGACTACATACCTGAAAACTCAGGGGACATCCCTT
  GCTGCAGCAGCACCTTCCACCCTGAGGAACATTTCTGCAGAGACAACACCTTGGAAGAGA
  ATCCGGGCCTTTCTTGGACTGATTGGCCAGAACCTTCCTTATTAGAAAAGCCAGAGTCCT
  CTCGTGGCAGGAGGAGCTACTCCTGGAGGTCCAAGGGTGTCAGAATCACTAACAGCTGCA
  AAAAATCCAAGTAACACCTTCTGAGTAGCTGCTGTCCCACACAATAGGGTATGAAGCTGC
  GCTCCTCCATCGGGTTTGGGGAGGGAGCACTCTGGGACTGTGAGACAAGGAAGCAGGGCC
  AGCAGTGAGACTATGAGCCAAGCAAAGAGAAGTCTCAGTGGAGCATGAGGAGGGAGCAGT
  CCAGATGCCAACAAGGAAATGCGTTTATGGCTACAAGAGTGCCTCTGCTTTCTCCTCCTC
  TCCTCCCACCAAGGATTCTTCCACCTTAATCTTGTTTTCATATGCCTCTTCTTACTTCAC
  CCATGTTTGTTGTTATGCAAATAAAGGTTTTCTCTCCAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016483 unedited
  NCAGTCAGTATTTGTATACGACNACTATAGGCGGCCGCCCGGTAGTAGCTACCACGGCGC
  TGTGTCAACGACTAAAGCTCCAGTACAGCGGCGCCCTCAGACAGCTGGGAGGGTGGCTCT
  GGCCGGGAGCGGCGGCCGGTGAGCTACCGCGAGGAGGAGCGGCGGAGGCGACCTCGGCCC
  GGCCCTGCACTGGCCGCCCGGCAGGCGCGACATGAGCCTGGTCTGGCATCCGCGGGATGC
  TCCTTAAGCCCCTTCTCCGGCTGTTAACCTCCGGGGAACGGTTGTGACCACACCGACACG
  TATTTTACAGATAAATCATTCTTGCGGCGGCGGGTCGAACACGTTTATTTATTTTTTATT
  TTCTCAACAAGCTTTTACCCAGCACCTGTCCAGTGAAACAACTTGATAATCGTTTCGAGG
  GGCGTCCGCCGGGTTAGGAAGCCACTGCCTGGCAGCTTGTGGAAGCCTCATTTGCAAAGC
  CACCCCTCAGATGTTTTGAAGATCGTGACGTCTTGTAACTAGCAGTGTGTGCACAGAATC
  CTACTCAAGGAACGTCTTGGCCCAGCGATGCAAAGAACTGAAGTTTCAAGCTGGAAGAGC
  CTGTATTGTCCTCACAATAGTATAGAAGAATTCAAGAGAGGAGAGAGAGACAGCACCGAA
  TGAAGACTGTAAAAGAAAAGAAGGAATGCCAGAGATTGAGAAAATCTGCCAAGACTAGGA
  GGGTAACCCAGAGGAAACCGTCTTCAGGGCCTGTTTGCTGGCTATGCCTTCGAGAACCTG
  GGGATCCCGAAAAATAGGGGAATTTCTTCAGAAAGACATATCAGCGTGCATTATTTCTGT
  GCTATCTTATCTAGTAAGCTGCCTCAGAGGGGCCCGTCCCACANAGGGTTCCATGAATTT
  CTGCCCTGAGACATCAAAAAGAAGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016483
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016483.4, NP_057567.3
• RefSeq Size: 2290 bp
• RefSeq ORF: 1146 bp
• Locus ID: 51533
• UniProt ID: Q9BWX1
• Cytogenetics: 3p21.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. This gene is expressed in the testis in Sertoli cells but not germ cells. The protein encoded by this gene contains plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. The protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
  Transcript Variant: This variant (1) encodes the longer isoform (1).