PDE8B (NM_003719) Human Untagged Clone

CAT#: SC125479

PDE8B (untagged)-Human phosphodiesterase 8B (PDE8B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE8B
• Synonyms: ADSD; PPNAD3
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003719 edited
  ATGGGCTGCGCCCCCAGCATCCATGTCTCGCAGAGCGGCGTGATCTACTGCCGGGACTCG
  GACGAGTCCAGCTCGCCCCGCCAGACCACCAGCGTGTCGCAGGGCCCGGCGGCACCCCTG
  CCCGGCCTCTTCGTCCAGACCGACGCCGCCGACGCCATCCCCCCGAGCCGCGCGTCGGGA
  CCCCCCAGCGTAGCCCGCGTCCGCAGGGCCCGCACCGAGCTGGGCAGCGGTAGCAGCGCG
  GGTTCCGCAGCCCCCGCCGCGACCACCAGCAGGGGCCGGAGGCGCCACTGCTGCAGCAGC
  GCCGAGGCCGAGACTCAGACCTGCTACACCAGCGTGAAGCAGGTGTCTTCTGCGGAGGTG
  CGCATCGGGCCCATGAGACTGACGCAGGACCCTATTCAGGTTTTGCTGATCTTTGCAAAG
  GAAGATAGTCAGAGCGATGGCTTCTGGTGGGCCTGCGACAGAGCTGGTTATAGATGCAAT
  ATTGCTCGGACTCCAGAGTCAGCCCTTGAATGCTTTCTTGATAAGCATCATGAAATTATT
  GTAATTGATCATAGACAAACTCAGAACTTCGATGCAGAAGCAGTGTGCAGGTCGATCCGG
  GCCACAAATCCCTCCGAGCACACGGTGATCCTCGCAGTGGTTTCGCGAGTATCGGATGAC
  CATGAAGAGGCGTCAGTCCTTCCTCTTCTCCACGCAGGCTTCAACAGGAGATTTATGGAG
  AATAGCAGCATAATTGCTTGCTATAATGAACTGATTCAAATAGAACATGGGGAAGTTCGC
  TCCCAGTTCAAATTACGGGCCTGTAATTCAGTGTTTACAGCATTAGATCACTGTCATGAA
  GCCATAGAAATAACAAGCGATGACCACGTGATTCAGTATGTCAACCCAGCCTTCGAAAGG
  ATGATGGGCTACCACAAAGGTGAGCTCCTGGGAAAAGAACTCGCTGATCTGCCCAAAAGC
  GATAAGAACCGGGCAGACCTTCTCGACACCATCAATACATGCATCAAGAAGGGAAAGGAG
  TGGCAGGGGGTTTACTATGCCAGACGGAAATCCGGGGACAGCATCCAACAGCACGTGAAG
  ATCACCCCAGTGATTGGCCAAGGAGGGAAAATTAGGCATTTTGTCTCGCTCAAGAAACTG
  TGTTGTACCACTGACAATAATAAGCAGATTCACAAGATTCATCGTGATTCAGGAGACAAT
  TCTCAGACAGAGCCTCATTCATTCAGATATAAGAACAGGAGGAAAGAGTCCATTGACGTG
  AAATCGATATCATCTCGAGGCAGTGATGCACCAAGCCTGCAGAATCGTCGCTATCCGTCC
  ATGGCGAGGATCCACTCCATGACCATCGAGGCTCCCATCACAAAGGTTATAAATATAATC
  AATGCAGCCCAAGAAAACAGCCCAGTCACAGTAGCGGAAGCCTTGGACAGAGTTCTAGAG
  ATTTTACGGACCACAGAACTGTACTCCCCTCAGCTGGGTACCAAAGATGAAGATCCCCAC
  ACCAGTGATCTTGTTGGAGGCCTGATGACTGACGGCTTGAGAAGACTGTCAGGAAACGAG
  TATGTGTTTACTAAGAATGTGCACCAGAGTCACAGTCACCTTGCAATGCCAATAACCATC
  AATGATGTTCCCCCTTGTATCTCTCAATTACTTGATAATGAGGAGAGTTGGGACTTCAAC
  ATCTTTGAATTGGAAGCCATTACGCATAAAAGGCCATTGGTTTATCTGGGCTTAAAGGTC
  TTCTCTCGGTTTGGAGTATGTGAGTTTTTAAACTGTTCTGAAACCACTCTTCGGGCCTGG
  TTCCAAGTGATCGAAGCCAACTACCACTCTTCCAATGCCTACCACAACTCCACCCATGCT
  GCCGACGTCCTGCACGCCACCGCTTTCTTTCTTGGAAAGGAAAGAGTAAAGGGAAGCCTC
  GATCAGTTGGATGAGGTGGCAGCCCTCATTGCTGCCACAGTCCATGACGTGGATCACCCG
  GGAAGGACCAACTTTTTCCTCTGCAATGCAGGCAGTGAGCTTGCTGTGCTCTACAATGAC
  ACTGCTGTTCTGGAGAGTCACCACACCGCCCTGGCCTTCCAGCTCACGGTCAAGGACACC
  AAATGCAACATTTTCAAGAATATTGACAGGAACCATTATCGAACGCTGCGCCAGGCTATT
  ATTGACATGGTTTTGGCAACAGAGATGACAAAACACTTTGAACATGTGAATAAGTTTGTG
  AACAGCATCAACAAGCCAATGGCAGCTGAGATTGAAGGCAGCGACTGTGAATGCAACCCT
  GCTGGGAAGAACTTCCCTGAAAACCAAATCCTGATCAAACGCATGATGATTAAGTGTGCT
  GACGTGGCCAACCCATGCCGCCCCTTGGACCTGTGCATTGAATGGGCTGGGAGGATCTCT
  GAGGAGTATTTTGCACAGACTGATGAAGAGAAGAGACAGGGACTACCTGTGGTGATGCCA
  GTGTTTGACCGGAATACCTGTAGCATCCCCAAGTCTCAGATCTCTTTCATTGACTACTTC
  ATAACAGACATGTTTGATGCTTGGGATGCCTTTGCACATCTGCCAGCCCTGATGCAACAT
  TTGGCTGACAACTACAAACACTGGAAGACACTAGATGACCTAAAGTGCAAAAGTTTGAGG
  CTTCCATCTGACAGCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003719 unedited
  GCACGAGGGGAAAGTTGGGGTGACGCGCGCGGTCCCCGGAGGCTCGGCGGGGGGCACCGC
  GGCCAGCCCGACGGAGCGGCGGACACACAGGCCGGGGGGCGCGCAGTCCGGGCGCCGCCG
  CGGCCGCCCCCTCACTGCAGGTGGCAGCGGGTGCGCTGGGTCCCGGCGGCCGCGGGCGCG
  GGCGGGCGCGCGGGGGAGCCCGGCCGAGGGATGGGCTGCGCCCCCAGCATCCATGTCTCG
  CAGAGCGGCGTGATCTACTGCCGGGACTCGGACGAGTCCAGCTCGCCCCGCCAGACCACC
  AGCGTGTCGCAGGGCCCGGCGGCACCCCTGCCCGGCCTCTTCGTCCAGACCGACGCCGCC
  GACGCCATCCCCCCGAGCCGCGCGTCGGGACCCCCCAGCGTAGCCCGCGTCCGCAGGGCC
  CGCACCGAGCTGGGCAGCGGTAGCAGCGCGGGTTCCGCAGCCCCCGCCGCGACCACCAGC
  AGGGGCCGGAGGCGCCACTGCTGCAGCAGCGCCGAGGCCGAGACTCAGACCTGCTACACC
  AGCGTGAAGCAGGTGTCTTCTGCGGAGGTGCGCATCGGGCCCATGAGACTGACGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003719 unedited
  AGCCCATATGGCGAATGGCCACTTTCCAGGCCCGGTAAAGCACTGGGGTAGGGTCACAGG
  GCATGCCACCCGGGTTCTGTTCAGAAAACAGCTATGACCGCGGCCGCAATCTATAGTCGA
  GTTTTTTTTTTTTTTTTTTAAAAAAAGCTTGTCCAAGGGCATTCAAATTTAATGGCTTTT
  ATATAATACTTGGTGTAGTGCCTCGTGGCTGCCTCTGTGAACCAAAAATAAAGGAAGCTC
  ATGGATTCCCCAAAAATGAAATGCCACTTTTTCCCTCATCATGGATGACTTTGTTAAGAT
  GAACCCCTTTTACAGGAAAGGGGTTACACAGGCTGCTGATACCAGTCTAGAGAGGGCACC
  CACCAGCCAAGGCTGTGTTCTAACTTAGGTGTCATACCCTCGGCCAAAATAACCATGTTG
  CCATAGAGGCTGTGAAGCTAACTAGTTTATCTGTAATTTGGTCCTAGTTGTTTCACTTAT
  TTTATGTCGTTTTTTTTTTTTCTTAAATAAATCTGTTCAGATAAGCTCCTTATGAATCCT
  TCATATCGATGTTCTTGAAGAAAACAGTCAAGCTAAACAGCAATGATGACTTTTATGGTA
  AAGGATGATCTGATCACTANCTAATCTACTTACTCACCATCCTGGGAGATGAGCTCACAG
  GCACCAAGGCTTTGCTTCCTGTGGCCTGCTAGTTACAGTGAACCATCTCCGTGGATTGAC
  AAGGGTACACAGGATGACAGCAGAGCAAAGGAGTTTGCCAAGTATTTGTTCTGTCATTAA
  GTATTCAAAAGAACATATTTTTTCTCCTCATGGAACATAACTTTCTAAAAATGAAATTTG
  GGGGACTTTGATGATTCAAGGTCAAATATTAACATTAGCTCCTTCACCATACCTGTTCAT
  GTATTAGAAAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003719
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003719.2, NP_003710.1
• RefSeq Size: 3570 bp
• RefSeq ORF: 2658 bp
• Locus ID: 8622
• UniProt ID: O95263
• Cytogenetics: 5q13.3
• Domains: PAS, PDEase
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: The protein encoded by this gene is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.