KCNMA1 (NM_001014797) Human Untagged Clone

CAT#: SC125444

KCNMA1 (untagged)-Human potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNMA1
• Synonyms: bA205K10.1; BKTM; CADEDS; hSlo; IEG16; KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA; SLO1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001014797, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAAATGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGAGGCAGCAGTCTTA
  GAATGAGTAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCCTCCTCCTCTTC
  CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC
  ATCCCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCCTGGCCTCCT
  CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT
  GTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATTAACAATGGCTCAAGCCAGGCGGATGGC
  ACTCTCAAACCAGTGGATGAAAAAGAGGAGGCAGTGGCCGCCGAGGTCGGCTGGATGACCTCCGTGAAGG
  ACTGGGCGGGGGTGATGATATCCGCCCAGACACTGACTGGCAGAGTCCTGGTTGTCTTAGTCTTTGCTCT
  CAGCATCGGTGCACTTGTAATATACTTCATAGATTCATCAAACCCAATAGAATCCTGCCAGAATTTCTAC
  AAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTTCTCTACTTCGGCTTGCGGTTTA
  TTGCAGCCAACGATAAATTGTGGTTCTGGCTGGAAGTGAACTCTGTAGTGGATTTCTTCACGGTGCCCCC
  CGTGTTTGTGTCTGTGTACTTAAACAGAAGTTGGCTTGGTTTGAGATTTTTAAGAGCTCTGAGACTGATA
  CAGTTTTCAGAAATTTTGCAGTTTCTGAATATTCTTAAAACAAGTAATTCCATCAAGCTGGTGAATCTGC
  TCTCCATATTTATCAGCACGTGGCTGACTGCAGCCGGGTTCATCCATTTGGTGGAGAATTCAGGGGACCC
  ATGGGAAAATTTCCAAAACAACCAGGCTCTCACCTACTGGGAATGTGTCTATTTACTCATGGTCACAATG
  TCCACCGTTGGTTATGGGGATGTTTATGCAAAAACCACACTTGGGCGCCTCTTCATGGTCTTCTTCATCC
  TCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATA
  CGGGGGCTCCTATAGTGCGGTTAGTGGAAGAAAGCACATTGTGGTCTGCGGACACATCACTCTGGAGAGT
  GTTTCCAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGACGTCAATGTGGAGATCGTTTTTCTTC
  ACAACATCTCCCCCAACCTGGAGCTTGAAGCTCTGTTCAAACGACATTTTACTCAGGTGGAATTTTATCA
  GGGTTCCGTCCTCAATCCACATGATCTTGCAAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTT
  GCCAACAAGTACTGCGCTGACCCGGATGCGGAGGATGCCTCGAATATCATGAGAGTAATCTCCATAAAGA
  ACTACCATCCGAAGATAAGAATCATCACTCAAATGCTGCAGTATCACAACAAGGCCCATCTGCTAAACAT
  CCCGAGCTGGAATTGGAAAGAAGGTGATGACGCAATCTGCCTCGCAGAGTTGAAGTTGGGCTTCATAGCC
  CAGAGCTGCCTGGCTCAAGGCCTCTCCACCATGCTTGCCAACCTCTTCTCCATGAGGTCATTCATAAAGA
  TTGAGGAAGACACATGGCAGAAATACTACTTGGAAGGAGTCTCAAATGAAATGTACACAGAATATCTCTC
  CAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTCAAGCTCCTAATG
  ATAGCCATTGAGTACAAGTCTGCCAACCGAGAGAGCCGAAGCCGAAAGCGTATATTAATTAATCCTGGAA
  ACCATCTTAAGATCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGC
  ATTTTTTTACTGCAAGGCCTGTCATGATGACATCACAGATCCCAAAAGAATAAAAAAATGTGGCTGCAAA
  CGGCTTGAAGATGAGCAGCCGTCAACACTATCACCAAAAAAAAAGCAACGGAATGGAGGCATGCGGAACT
  CACCCAACACCTCGCCTAAGCTGATGAGGCATGACCCCTTGTTAATTCCTGGCAATGATCAGATTGACAA
  CATGGACTCCAATGTGAAGAAGTACGACTCTACTGGGATGTTTCACTGGTGTGCACCCAAGGAGATAGAG
  AAAGTCATCCTGACTCGAAGTGAAGCTGCCATGACCGTCCTGAGTGGCCATGTCGTGGTCTGCATCTTTG
  GCGACGTCAGCTCAGCCCTGATCGGCCTCCGGAACCTGGTGATGCCGCTCCGTGCCAGCAACTTTCATTA
  CCATGAGCTCAAGCACATTGTGTTTGTGGGCTCTATTGAGTACCTCAAGCGGGAATGGGAGACGCTTCAT
  AACTTCCCCAAAGTGTCCATATTGCCTGGTACGCCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCA
  ACCTCTGTGACATGTGCGTTATCCTGTCAGCCAATCAGAATAATATTGATGATACTTCGCTGCAGGACAA
  GGAATGCATCTTGGCGTCACTCAACATCAAATCTATGCAGTTTGATGACAGCATCGGAGTCTTGCAGGCT
  AATTCCCAAGGGTTCACACCTCCAGGAATGGATAGATCCTCTCCAGATAACAGCCCAGTGCACGGGATGT
  TACGTCAACCATCCATCACAACTGGGGTCAACATCCCCATCATCACTGAACTAGTGAACGATACTAATGT
  TCAGTTTTTGGACCAAGACGATGATGATGACCCTGATACAGAACTGTACCTCACGCAGCCCTTTGCCTGT
  GGGACAGCATTTGCCGTCAGTGTCCTGGACTCACTCATGAGCGCGACGTACTTCAATGACAATATCCTCA
  CCCTGATACGGACCCTGGTGACCGGAGGAGCCACGCCGGAGCTGGAGGCTCTGATTGCTGAGGAAAACGC
  CCTTAGAGGTGGCTACAGCACCCCGCAGACACTGGCCAATAGGGACCGCTGCCGCGTGGCCCAGTTAGCT
  CTGCTCGATGGGCCATTTGCGGACTTAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAGCTCTGA
  AAACATATAATATGCTTTGTTTTGGAATTTACCGGCTGAGAGATGCTCACCTCAGCACCCCCAGTCAGTG
  CACAAAGAGGTATGTCATCACCAACCCGCCCTATGAGTTTGAGCTCGTGCCGACGGACCTGATCTTCTGC
  TTAATGCAGTTTGACCACAATGCCGGCCAGTCCCGGGCCAGCCTGTCCCATTCCTCCCACTCGTCGCAGT
  CCTCCAGCAAGAAGAGCTCCTCTGTTCACTCCATCCCATCCACAGCAAACCGACAGAACCGGCCCAAGTC
  CAGGGAGTCCCGGGACAAACAGAACAGAAAAGAAATGGTTTACAGATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001014797 unedited
  AGTGCGGCCGCGACCTCGGCACGAGGCAGCAGTAGCAGCACTCTGGCCGTTGACTGGTGT
  TTGGGGTGGCGCCGGCGGCGGGAGGATCTCCCCCGGGCCCCTGCGCCGGCTGCCCGTTGC
  TAGCTACTGGCAAATGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGC
  GGAGGCAGCAGTCTTAAAATGAGTAGCAATATCCACGCGAACCAATCTCAGCCTAAACGC
  GTCCTCTTCCTCCTCCTCCTCCTCTTCCTTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCT
  CGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATCATCCCGGTGACCATGGAGGTGC
  CGTGCGACAGCCGGGGCCAACGCATNGTGGTGGGCTTTCCTGGCCTCCTCCATGGTGACT
  TTCTTNCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT
  GTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGAATAACAATGGCTCAAGCCA
  GNCGGATGGCACTCTCAAACCAGTGGATGAAAAAGAGNANGCAGTGGCGNCCCGAGGTCG
  CTTGGATGACCTCCGTGAAAGACTGGCCCNNGGGGGGNTGATGATATCCGCCCAGACACT
  GACTGGGCAAAGCCCCGNGGNGTGGCTTAAATCCTTTGCTCTCACCATCGGGGGCACTTG
  GTAATATACTTCCATAGGATTTCATCAAACCCAAATAGAATNCTGNNCAGAATTNCTACN
  NAAGATTCACATTNACAGATCGACATGGCTTTNAAAGCGGGTTCTTCTTCTTACTTGGGG
  GGGGGGGGGTATTGAGACATAAAAAAAGGGGGTCTGGGCGGGGAGGGACTTTTTAAGGGA
  TTTTTTAGGGCCCCCCCGTTGTTTTTGTTGGACTAAAACAAAGGGGCTTGGGGTGGAAAT
  TTAAACCTTGACGGACAATTTCAAAAATTGGGGGTTTGAATTTTAAAAAGATCCTTAACG
  GGGAATGCTCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001014797 unedited
  TTCGGATTGTTTTTACTAATTTATTTTCCCTTATTACAATTAATTGTGGTGGTGACCATC
  ATTCTCCTCAAAGAAAAAGAGAGAAAGAAGAGAAAAACACATTAAGGAGTCTGACCACTT
  ATTCATTTAGATTTTGCATTGTTTAGATATAGGGAAGATATTTGACAATGAAAAGAAAAA
  ATATATTTTCTTTCTAATAAATAAAAATAATTTAAAATGCTAGAGGCTATACATGACTAT
  TTTAGTATGTTCATTTATAAATGAAATCATTTTCTTTTCCAAAATACATGGAAGCAATAT
  TCAAGGCAGACAAAGAGAAAGATCTGTCTACTTTATTTTCTGGCAATTCTTGCTTATCCT
  AACGTTTCCACCAGTGATTCAGCATGTAAGAAGAAAAGTCACATCACCATTTATGAAGTT
  TGTCAGGCTTTAAAAAAATAGTTCTGGTCTCCTGGGAGTCAACATTCATCTTCAACTTCT
  CTGATTGGTGGAATCAAGCTGCTTGTGGATTTATATTGGTTGATCTGGTTAGCCATGTGG
  GGTACTCATGGGCTTGATTTGAATGTTTCTGGGATAGGCATTATCCGGTTCATCTTGTAA
  CCATTTCTTTTTTCTGTTTGTCCCGGGACTCCCCGGACTTGGGCCCGTTCTGTCCGGTTT
  GCTGTGGATGGGGATGGAGTGACAAAGGGACTTTTCTTGCTGAGGACTGCGACGAGTGGA
  AGGATGGAACAAGCTTGCCCGGGCCGGGCCGGCTTGTGGCAAACCTGCTTAAACAAAAAA
  CAGGTCCCTCTGGCCCAGCCTAACCCTCAAGGCCGGTTGGGGATTAACTACCCCTTTGTG
  CCCCGCCCGGGGGGCCTTGGGGGAGATTCCTTACCCGGGAAATTCCAAACACAACATTTT
  TATTGTTTCTGAGTTTTGCGGAAAATTCCCTTACCCCCCCTCTCCCTTAGCTCCGATGTG
  CCCCTAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001014797
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014797.1, NP_001014797.1
• RefSeq Size: 11993 bp
• RefSeq ORF: 3549 bp
• Locus ID: 3778
• UniProt ID: Q12791
• Cytogenetics: 10q22.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.