Synaptojanin 2 (SYNJ2) (NM_003898) Human Untagged Clone

CAT#: SC125373

SYNJ2 (untagged)-Human synaptojanin 2 (SYNJ2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Synaptojanin 2
• Synonyms: INPP5H
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125373 sequence for NM_003898 edited (data generated by NextGen Sequencing)
  ATGGCCCTGAGCAAAGGGCTGCGGCTGCTGGGGCGCCTGGGGGCCGAGGGGGACTGTAGC
  GTGCTGCTGGAGGCGCGCGGCCGCGACGACTGCCTGCTGTTCGAGGCCGGCACGGTGGCC
  ACGCTGGCTCCAGAAGAAAAGGAAGTCATTAAAGGACAGTATGGCAAGCTCACGGACGCG
  TACGGCTGCCTGGGGGAGCTGAGGCTGAAATCTGGTGGCACGTCTCTGAGCTTCCTGGTG
  TTGGTGACAGGCTGCACATCTGTGGGCAGAATTCCAGATGCTGAAATCTACAAAATCACT
  GCCACTGACTTTTACCCTCTTCAGGAAGAGGCCAAGGAGGAGGAACGCCTCATAGCTTTG
  AAGAAAATCCTCAGCTCGGGGGTGTTCTATTTCTCATGGCCAAACGATGGGTCTCGCTTT
  GACCTGACTGTCCGCACGCAGAAGCAGGGGGATGACAGCTCTGAATGGGGGAACTCCTTC
  TTCTGGAACCAGCTGTTGCACGTGCCCTTGAGGCAGCACCAGGTGAGCTGCTGTGACTGG
  CTGCTGAAGATCATCTGCGGGGTGGTCACCATCCGCACCGTGTATGCCTCCCACAAGCAG
  GCCAAGGCCTGCCTCGTCTCTCGCGTTAGCTGTGAGCGCACAGGCACTCGCTTCCACACC
  CGTGGCGTGAACGACGACGGCCATGTGTCCAACTTCGTGGAGACAGAGCAGATGATTTAC
  ATGGACGATGGAGTGTCATCTTTTGTCCAGATCAGAGGCTCCGTTCCGCTGTTCTGGGAA
  CAGCCAGGGCTTCAGGTTGGCTCCCATCATCTGAGACTCCACAGAGGCCTGGAAGCCAAT
  GCCCCTGCTTTCGACAGGCACATGGTGCTTCTGAAGGAGCAGTACGGGCAGCAGGTGGTC
  GTGAACCTTCTGGGAAGCAGAGGCGGAGAGGAGGTGCTCAACAGAGCCTTCAAGAAGCTG
  CTCTGGGCTTCTTGCCACGCGGGCGACACGCCTATGATCAATTTTGACTTCCATCAGTTT
  GCCAAAGGTGGGAAGCTAGAGAAATTGGAGACCCTCTTGAGGCCACAGTTAAAGCTGCAC
  TGGGAAGACTTCGATGTGTTCACAAAGGGGGAGAACGTCAGTCCACGTTTTCAGAAAGGC
  ACTTTGCGGATGAACTGTCTTGACTGCCTGGACCGAACCAACACTGTGCAGAGCTTCATC
  GCGCTCGAGGTCCTGCATCTGCAGCTCAAGACCCTGGGGCTGAGTTCAAAACCCATCGTT
  GACCGCTTTGTGGAGTCCTTCAAAGCCATGTGGTCTCTGAATGGCCACAGCCTGAGCAAG
  GTGTTCACAGGCAGCAGAGCCCTGGAAGGGAAGGCCAAGGTGGGGAAGCTGAAGGATGGA
  GCCCGGTCCATGTCTCGAACCATCCAGTCCAACTTCTTCGACGGGGTGAAGCAGGAGGCC
  ATCAAGCTGCTGCTGGTTGGGGACGTCTACGGCGAGGAGGTGGCAGACAAAGGGGGCATG
  CTGCTGGACAGCACGGCGCTCCTGGTGACTCCCAGGATCCTGAAAGCTATGACTGAGCGT
  CAGTCCGAATTCACAAATTTCAAGCGGATCCGGATTGCTATGGGGACCTGGAACGTGAAC
  GGAGGAAAGCAGTTCCGGAGCAACGTGCTCAGGACGGCGGAGCTGACAGACTGGCTGCTC
  GACTCGCCCCAGCTCTCGGGAGCTACCGACTCCCAGGATGACAGCAGCCCAGCTGACATA
  TTTGCTGTGGGGTTTGAAGAGATGGTGGAATTGAGCGCAGGGAATATTGTCAATGCCAGT
  ACTACCAACAAGAAGATGTGGGGTGAACAGCTTCAGAAAGCCATCTCACGCTCTCATAGA
  TACATTCTGTTGACTTCGGCACAGCTGGTGGGCGTCTGTCTTTATATCTTTGTACGTCCA
  TACCATGTCCCGTTCATCAGGGACGTAGCCATCGACACAGTGAAGACGGGCATGGGGGGC
  AAGGCGGGGAACAAGGGCGCCGTCGGCATCCGCTTCCAGTTCCACAGCACCAGCTTCTGC
  TTCATATGTAGTCACCTGACGGCCGGGCAGTCCCAGGTGAAGGAGCGGAATGAAGACTAC
  AAGGAGATCACCCAGAAACTCTGCTTCCCAATGGGGAGAAATGTTTTTTCTCATGATTAT
  GTATTTTGGTGTGGCGATTTCAACTACCGCATTGATCTTACTTATGAAGAAGTCTTCTAT
  TTTGTTAAACGCCAAGACTGGAAGAAACTTCTGGAATTTGATCAACTACAGCTACAGAAA
  TCAAGTGGAAAAATTTTTAAGGACTTTCACGAAGGAGCCATTAACTTTGGACCCACCTAC
  AAGTATGACGTCGGCTCAGCCGCCTACGATACAAGCGACAAATGCCGCACCCCCGCCTGG
  ACAGACAGGGTGCTGTGGTGGAGGAAGAAACATCCCTTTGATAAAACAGCTGGAGAACTC
  AACCTTCTAGACAGTGATCTAGATGTTGACACCAAAGTCAGACACACCTGGTCTCCTGGT
  GCCCTGCAGTATTATGGTCGTGCGGAGCTACAAGCTTCTGATCACAGACCTGTGCTGGCG
  ATCGTGGAGGTGGAAGTTCAGGAAGTCGATGTGGGTGCTCGGGAGAGGGTTTTCCAGGAA
  GTGTCCTCCTTCCAGGGCCCCCTGGATGCCACTGTTGTAGTAAACCTTCAATCACCGACC
  TTAGAAGAGAAAAACGAGTTTCCAGAGGACCTGCGTACTGAGCTCATGCAGACCTTGGGG
  AGTTATGGGACAATTGTTCTTGTCAGGATCAACCAAGGGCAGATGCTGGTAACTTTTGCA
  GACAGTCACTCGGCTCTCAGTGTCCTGGACGTGGACGGTATGAAGGTGAAAGGCAGAGCA
  GTGAAGATTAGACCGAAGACCAAGGACTGGCTGAAAGGTTTGCGAGAGGAGATCATTCGG
  AAACGAGACAGCATGGCCCCCGTGTCTCCCACTGCCAACTCCTGTTTGCTGGAGGAAAAC
  TTTGACTTCACAAGTTTGGACTATGAGTCAGAAGGGGATATTCTTGAAGACGATGAAGAC
  TACTTGGTGGATGAATTCAATCAGCCTGGGGTCTCGGACAGTGAACTCGGGGGAGACGAC
  CTCTCTGATGTCCCCGGCCCCACAGCACTGGCTCCTCCCAGCAAGTCACCTGCTCTCACC
  AAAAAGAAGCAGCATCCAACGTACAAAGATGACGCGGACCTGGTGGAGCTCAAGCGGGAG
  CTGGAAGCCGTCGGGGAGTTCCGCCACCGTTCTCCGAGCAGGTCTCTGTCGGTCCCCAAC
  CGACCTCGGCCACCTCAACCCCCGCAGAGACCCCCCCCTCCAACCGGTTTAATGGTGAAA
  AAGTCGGCTTCAGATGCGTCCATCTCCTCCGGCACCCATGGGCAGTATTCAATTTTGCAG
  ACGGCAAGACTTCTACCAGGAGCACCTCAGCAACCTCCCAAGGCTCGGACTGGAATAAGT
  AAACCTTATAATGTCAAGCAGATCAAAACCACCAATGCCCAGGAGGCAGAAGCAGCAATC
  CGGTGTCTCCTGGAAGCCAGAGGAGGTGCCTCCGAAGAAGCCCTAAGTGCCGTGGCCCCA
  AGGGACCTTGAAGCATCCTCTGAACCAGAGCCCACACCGGGGGCAGCCAAACCAGAGACC
  CCACAGGCGCCCCCACTCCTTCCCCGTCGGCCCCCACCCAGAGTTCCTGCCATCAAGAAG
  CCAACCTTGAGAAGGACAGGAAAGCCCCTGTCACCGGAAGAACAGTTTGAGCAACAGACT
  GTCCATTTTACAATCGGGCCCCCGGAGACAAGCGTTGAGGCCCCTCCTGTCGTGACAGCC
  CCTCGAGTCCCTCCTGTTCCCAAACCAAGAACATTTCAGCCTGGGAAAGCTGCAGAGAGG
  CCAAGCCACAGGAAGCCAGCATCAGACGAAGCCCCTCCTGGGGCAGGAGCCTCTGTGCCA
  CCACCTCTGGAGGCGCCGCCTCTTGTGCCCAAGGTACCCCCGAGGAGGAAGAAGTCAGCC
  CCCGCAGCCTTCCACCTGCAGGTCCTGCAGAGCAACAGCCAGCTTCTCCAGGGCCTCACT
  TACAATAGCAGTGACAGCCCCTCTGGGCACCCACCTGCCGCGGGCACCGTCTTCCCACAA
  GGGGACTTTCTCAGCACTTCATCTGCTACAAGCCCCGACAGCGATGGCACCAAAGCGATG
  AAGCCAGAGGCAGCCCCACTTCTTGGTGATTATCAGGACCCCTTCTGGAACCTTCTTCAC
  CACCCTAAACTGTTGAATAACACTTGGCTTTCTAAGAGCTCAGACCCTTTGGACTCAGGA
  ACCAGGAGCCCCAAAAGAGATCCCATAGACCCAGTGTCAGCTGGCGCTTCAGCTGCCAAG
  GCAGAGCTGCCACCAGATCATGGACACAAAACCTTAGGTCACTGGGTGACAATCAGTGAC
  CAAGAAAAGAGGACAGCACTGCAGGTGTTTGACCCACTGGCAAAAACATGA
  
  Clone variation with respect to NM_003898.3
  2352 t=>c;2556 g=>t;3090 a=>g;3303 g=>a;3402 a=>g;4403 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003898 unedited
  GAAAGGTTGTAGACTTTGAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGC
  GGCGCAAAGTGAAACTCTGGCCAAGTTGCCGGCCGCGCCGGGGAGCTTGTCGCGGNGCAA
  GCGCCGCCCTTCGGGGAGGACGTGGCCCCNNCGGCCCCCGCCCGCAGTGNGGCCCCGACC
  CTCATGGCCCTGAGCAAAGGGCTGCGGCTGCTGGGGCGCCTGGGGGCCGAGGGGGACTGT
  AGCGTGCTGCTGGAGGCGCGCGGCCGCGACGACTGCCTGCTGTTCGAGGCCGGCACGGTG
  GCCACGCTGGCTCCAGAAGAAAAGGAAGTCATTAAAGGACAGTATGGCAAGCTCACGGAC
  GCGTACGGCTGCCTGGGGGAGCTGAGGCTGAAATCTGGTGGCACGTCTCTGAGCTTCCTG
  GTGTTGGTGACAGGCTGCACATCTGTGGGCAGAATTCCAGATGCTGAAATCTACAAAATC
  ACTGCCACTGACTTTTACCCTCTTCAGGAAGAGGCCAAGGAGGAGGAACGCCTCATAGCT
  TTGAAGAAAATCCTCANGCTCGGGGTGTTCTATTTCTCATGGCCAAACGATGGGTCTCGC
  TTTGACCTGACTGTCCGCACGCAGAAGCCAGGGGATGACAGCTCTGAATGGNGGAACCTC
  CTCTTCTGGAACCAGCTGTTGCACGTGCNCTTGAGGCAGCACCAGGTGAGCTGCTGTGAC
  TTGCTGCTGAAGATCATCTGCGGGGTGGTCACCATTCCGACGTGTATGCCNTCCACAAGC
  AGGCCAAGGCTTGCCTCGTTTCTCGCGTTACCTGTGACCCCCAGGCCCTTCGTTCCACAA
  CCGTGCCTTGAAAACACCGGCCATGTTCAAATTCGGGAAACAGACAATATTTAAATGCCC
  CATGGAGGGCACCTTTGTCATTATAAAGGCTCCTTCCCTTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003898 unedited
  ACTTTCTGAGGCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTT
  CAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAT
  CCTTTTCCAGGTATTAGTTTTCAGATGAAAAGAGATACGTGTTGTAACTTTTGTTTTGAT
  GGCCATATTTAAGTAATCTGCAATATATCTTGACAGTGACAATGAAAACTAATTATGAAA
  TTCCAGATGTCTAATTGGTAACAAGACAGTGGCATAGCCCAGGCAAGATGATCCTTACTA
  TCCTAGCAGTCAGCTTTTAGGACAGACATCCCCCAGTCACGTAAGGGATGACACTTGATC
  ACTGCTGTGCGAGCCTCTGTACGAGGCTCCCAGATACAACAGGCGGTACAGTAAGATTTC
  ACTACTGAACTTCTTATTAAATAGCGAAAACACGGTATCTGCTCCAAATGGATTCCAGAC
  CTTGGTACATTTACTTTAAAAATGTAAAAGCACTTTGGGTAATGTCTTTTTTCAGTTTTT
  TGAAATAGGGTGAAGAAAGTGCCTGTTTGGGAAAACGTTGGGGAAAAAAATTGTTATAGG
  GCAAAAAAAAAATATTGGACCCAAACCATTATTGGGACAAAGGCAAAAACCCCAAGGAAA
  ACAACTTGGCCCTTGGGTGGATTCCTTAATATTGCCAGGGGGACACCATAAGCAACTTTT
  TCCAATCCCGGAAGCTTCCAGACCCGGGACAAGACCAAGGCCTTGAAAACAATTCTTCCA
  TATATTCTAAACCATGGAGAAATAAATTATCCGCGTATTCCAACCAAGAGTTTTTCGAAC
  TGGGGGGCCTTTTTATGTGGAAAG
  
• Restriction Sites: Please inquire
• ACCN: NM_003898
• Insert Size: 5700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003898.2, NP_003889.1
• RefSeq Size: 6738 bp
• RefSeq ORF: 4491 bp
• Locus ID: 8871
• UniProt ID: O15056
• Cytogenetics: 6q25.3
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: The gene is a member of the inositol-polyphosphate 5-phosphatase family. The encoded protein interacts with the ras-related C3 botulinum toxin substrate 1, which causes translocation of the encoded protein to the plasma membrane where it inhibits clathrin-mediated endocytosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). This variant (1) is also known as synaptojanin 2B or 2B2 (SJ2B2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.