Cortactin (CTTN) (NM_138565) Human Untagged Clone

CAT#: SC124848

CTTN (untagged)-Human cortactin (CTTN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cortactin
• Synonyms: EMS1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_138565, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGAAAGCTTCAGCAGGCCACGCTGTGTCCATCGCCCAGGATGACGCGGGGGCCGATGACTGGGAGA
  CCGACCCTGATTTTGTGAATGATGTGAGTGAGAAGGAGCAAAGATGGGGTGCCAAGACGGTGCAGGGCTC
  CGGGCACCAGGAGCATATCAACATACACAAGCTGAGGGAGAATGTCTTTCAAGAGCATCAGACCCTTAAG
  GAGAAGGAACTTGAAACAGGACCAAAAGCTTCCCATGGCTATGGAGGGAAATTTGGTGTGGAACAAGACC
  GAATGGATAAGTCAGCTGTCGGCCACGAATATCAGTCGAAACTTTCCAAGCACTGCTCGCAGGTGGACTC
  GGTCCGTGGCTTCGGAGGCAAGTTTGGTGTCCAGATGGACAGAGTTGATCAGTCTGCTGTAGGCTTTGAA
  TACCAGGGGAAGACTGAGAAGCATGCCTCCCAGAAAGACTACTCCAGTGGTTTTGGCGGCAAGTATGGCG
  TGCAGGCCGACCGAGTAGACAAGAGCGCGGTGGGCTTCGACTACCAGGGCAAGACGGAGAAGCACGAGTC
  ACAGAGAGATTACTCCAAAGGTTTCGGCGGCAAATACGGTATCGACAAGGACAAAGTGGATAAGAGCGCC
  GTTGGCTTTGAGTATCAAGGCAAAACGGAGAAGCACGAGTCCCAGAAAGACTATGTGAAAGGGTTTGGAG
  GAAAATTTGGTGTGCAGACAGACAGACAAGACAAATGTGCCCTTGGCTGGGATCACCAGGAGAAATTGCA
  GCTGCATGAATCCCAAAAAGACTACTCCAAAGGATTCGGCGGGAAGTATGGGGTGCAGAAGGATCGGATG
  GATAAGAATGCGTCAACCTTTGAGGATGTCACCCAGGTGTCCTCTGCCTACCAGAAGACAGTACCTGTCG
  AAGCTGTGACCAGCAAAACAAGTAACATCAGAGCTAACTTTGAAAACCTCGCTAAGGAGAAAGAGCAGGA
  GGACAGGCGGAAGGCGGAGGCGGAGAGAGCCCAGCGGATGGCCAAGGAGCGGCAGGAGCAGGAAGAGGCC
  AGGAGGAAGCTGGAGGAGCAAGCCAGAGCCAAAACGCAAACGCCCCCTGTGTCGCCCGCACCTCAGCCAA
  CCGAGGAGAGGCTGCCCTCGAGCCCCGTCTATGAGGATGCGGCTTCCTTCAAGGCAGAGCTGAGCTACAG
  AGGCCCTGTGAGTGGGACGGAGCCGGAGCCCGTGTACAGCATGGAGGCCGCTGACTACCGAGAGGCCAGC
  AGCCAGCAGGGCCTGGCCTATGCCACAGAGGCTGTCTATGAAAGCGCAGAGGCCCCGGGCCACTATCCCG
  CAGAGGACAGCACCTACGATGAGTACGAGAACGATCTGGGGATCACAGCCGTCGCCCTGTACGACTACCA
  GGCTGCGGGCGATGATGAGATCTCATTTGACCCTGATGACATCATCACCAACATCGAGATGATTGACGAC
  GGCTGGTGGCGCGGGGTGTGCAAGGGCCGGTACGGGCTCTTCCCAGCCAACTATGTGGAGCTGCGGCAGT
  AG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138565 unedited
  NGGTTCAGATTTTGTAATACGACTTACTATAGNNGGCGGCCGCGNNNAATTCGGCACGAA
  GGCGGNAATCCAGGGGCCGACCCGGGCCGGACCGACCCCAGGCGGCGACGGAATCAGTCC
  CCAATGCCTGGAAATTCCTCATTGGATTACTGTGTTTTAAACAGAATTTCGTGAACAGCC
  TTTTATCTCCAAGCGGAAAGAAAGATGTGGAAAGCTTCAGCAGGCCACGCTGTGTCCATC
  GCCCAGGATGACGCGGGGGCCGATGACTGGGAGACCGACCCTGATTTTGTGAATGATGTG
  AGTGAGAAGGAGCAAAGATGGGGTGCCAAGACGGTGCAGGGCTCCGGGCACCAGGAGCAT
  ATCAACATACACAAGCTGAGGGAGAATGTCTTTCAAGAGCATCAGACCCTTAAGGAGAAG
  GAACTTGAAACAGGACCAAAAGCTTCCCATGGCTATGGAGGGAAATTTGGTGTGGAACAA
  GACCGAATGGATAAGTCAGCTGTCGGCCACGAATATCAGTCGAAACTTTCCAAGCACTGC
  TCGCAGGTGGACTCGGTCCGTGGCTTCGGAGGCAAGTTTGGTGTCCAGATGGACAGAGTT
  GATCAGTCTGCTGTAGGCTNTGAATACCAGGGGAAGACTGAGAAGCATGCCTCCCAGAAA
  GACTACTCCAGTGGTTTTGGCGGCAAGTATGGCGTGCAGGCCGACCGAGTAGACAAGAGC
  GCGGTGGGCTTCGACTACCAGGGGCAGACGGAGAAGCACGAGTCACAGAGAGATTACTCN
  CAAGGGTTCNGCGGGCAATACCGTATCGACAGGNACCCAAGTGGATAGAGCGCCGTTGGC
  TTTGAGTATCAAGGCAAAACGGNAGAGCACGAGTCCCAGAAAGACTATGTGAAAAGGGTT
  TGGNAGAANAATTGTGTGCAGACAGACCAGACAGACAATGTGCCCTTGCCTGGNATCACA
  GNAGAAATGCAGCTGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138565
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138565.1, NP_612632.1
• RefSeq Size: 3176 bp
• RefSeq ORF: 1542 bp
• Locus ID: 2017
• UniProt ID: Q14247
• Cytogenetics: 11q13.3
• Protein Families: Druggable Genome
• Protein Pathways: Pathogenic Escherichia coli infection, Tight junction
• Gene Summary: This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (2) lacks an internal segment of sequence, compared to variant 1. The resulting protein (isoform b) has the same N- and C- termini but is shorter when it is compared to isoform a.