TGIF (TGIF1) (NM_173209) Human Untagged Clone

CAT#: SC124492

TGIF1 (untagged)-Human TGFB-induced factor homeobox 1 (TGIF1), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TGIF1
• Synonyms: HPE4; TGIF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_173209, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACATTCCCTTGGACCTTTCTTCATCCGCTGGCTCAGGCAAGAGAAGGAGAAGGGGCAACCTACCCA
  AGGAGTCTGTGCAGATTCTTCGGGATTGGCTGTATGAGCACCGTTACAATGCCTATCCTTCAGAGCAAGA
  AAAAGCGTTGCTGTCCCAGCAAACACACCTGTCTACGCTACAGGTCTGTAACTGGTTCATCAACGCCCGC
  CGCAGGCTCCTCCCTGACATGCTGAGAAAGGATGGCAAAGATCCAAATCAGTTCACAATTTCCCGCCGTG
  GGGCCAAGATTTCTGAAACGAGCTCTGTGGAGTCCGTGATGGGCATCAAAAACTTCATGCCAGCTCTAGA
  GGAGACCCCATTTCATTCCTGTACAGCTGGGCCAAACCCAACCCTAGGGAGGCCACTGTCTCCTAAGCCG
  TCATCCCCGGGATCAGTTTTGGCTCGTCCATCAGTGATCTGCCATACCACTGTGACTGCATTGAAAGATG
  TCCCTTTCTCTCTCTGCCAGTCGGTCGGTGTGGGACAAAACACAGATATACAGCAGATAGCGGCCAAAAA
  CTTCACAGACACCTCTCTCATGTACCCAGAGGACACTTGTAAATCTGGACCAAGTACGAATACACAGAGT
  GGTCTTTTCAACACTCCTCCCCCTACTCCACCGGACCTCAACCAGGACTTCAGTGGATTTCAGCTTCTAG
  TGGATGTTGCACTCAAACGGGCTGCAGAGATGGAGCTTCAGGCAAAACTTACAGCTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173209 unedited
  CATTTCAGATTTTGTATACGACTTACTATAGGGCGGCCGCGATTCGGCACCAGAAGAGTT
  GGTTCTTGTCTCGAAACTTTTCCTCCCGTGCCCCGCCCCTTGAATCAGAGGCGGCTGTCT
  CGTGCGGCTAGAGCAGGGCCAGTAGAGTTCGGGGCAGCTGTCAAAAACGAGACGGGGAGG
  CTTTTCTTCCCGGGGGTGGAGGAGGGAAGGAGGGAGGTGTCCTTTTTTCCATCTGTTGCA
  GTTTCCGAACCAAACGCACCCTCGCCAGCCCCGGGAAGAAAGGCCCAAGGGAGAAACCTG
  GGGCGCTCGGCTGGTGCCCCCACCGCCGGGCGAGGGATGCGGGCGGTCCGTGCGCGGCAG
  GCGGAAAGGATCGTGTTTCTGGACCCGGCCACCCCCCGCGTCCGCACCGCCGCCCCCGAG
  GGACGAGTGACAGCGGCCGCCGCGCTCGGTCCAGTCTTCCCGGCTGGAAGGTATTGTTGC
  AGCATCTGGCAGTGAGACTGAGGATGAGGACAGCATGGACATTCCCTTGGACCTTTCTTC
  ATCCGCTGGCTCAGGCAAGAGAAGGAGAAGGGGCAACCTACCCAAGGAGTCTGTGCAGAT
  TCTTCGGGATTGGCTGTATGAGCACCGTTACAATGCCTATCCTTCAGAGCAAGAAAAAGC
  GTTGCTGTCCCAGCAAACACACCTGTCTACGCTACAGGTCTGTAACTGGTTCATCAACGC
  CCGCCGCAGGCTCCTCCCTGACATGCTGAGAAAGGATGCCACAGATCCAAATCAGTTCAC
  AATTTCCCGCCGTGGGGCCACGATTCTGAACGAGCTCTGTGGAGTCCCGGATGGGCATTA
  AAAACTTAATGCAGCTCTATAGAGACCCCGTTTCATTCTGTACAGCTGGCCAAACCAACC
  CTAGGTAGGCACTGGCTCCTAAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_173209
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173209.1, NP_775301.1
• RefSeq Size: 1382 bp
• RefSeq ORF: 759 bp
• Locus ID: 7050
• UniProt ID: Q15583
• Cytogenetics: 18p11.31
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Gene Summary: The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (5) has an alternate 5' terminal exon, which results in a different 5' UTR and a downstream translation start codon, compared to variant 1. The resulting isoform (d) has a shorter N-terminus, compared to isoform a. Variants 5, 6, 7, 8, and 11 encode the same isoform d. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.