SEMA6D (NM_153617) Human Untagged Clone

CAT#: SC124439

SEMA6D (untagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEMA6D
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_153617, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGGTCTTCCTGCTTTGTGCCTACATACTGCTGCTGATGGTTTCCCAGTTGAGGGCAGTCAGCTTTC
  CTGAAGATGATGAACCCCTTAATACTGTCGACTATCACTATTCAAGGCAATATCCGGTTTTTAGAGGACG
  CCCTTCAGGCAATGAATCGCAGCACAGGCTGGACTTTCAGCTGATGTTGAAAATTCGAGACACACTTTAT
  ATTGCTGGCAGGGATCAAGTTTATACAGTAAACTTAAATGAAATGCCCAAAACAGAAGTAATACCCAACA
  AGAAACTGACATGGCGATCAAGACAACAGGATCGAGAAAACTGTGCTATGAAAGGCAAGCATAAAGATGA
  ATGCCACAACTTTATCAAAGTATTTGTTCCAAGAAACGATGAGATGGTTTTTGTTTGTGGTACCAATGCA
  TTCAATCCCATGTGTAGATACTACAGGTTGAGTACCTTAGAATATGATGGGGAAGAAATTAGTGGCCTGG
  CAAGATGCCCATTTGATGCCAGACAAACCAATGTTGCCCTCTTTGCTGATGGGAAGCTGTATTCTGCCAC
  AGTGGCTGACTTCTTGGCCAGCGATGCCGTTATTTATCGAAGCATGGGTGATGGATCTGCCCTTCGCACA
  ATAAAATATGATTCCAAATGGATAAAAGAGCCACACTTTCTTCATGCCATAGAATATGGAAACTATGTCT
  ATTTCTTCTTTCGAGAAATCGCTGTCGAACATAATAATTTAGGCAAGGCTGTGTATTCCCGCGTGGCCCG
  CATATGTAAAAACGACATGGGTGGTTCCCAGCGGGTCCTGGAGAAACACTGGACTTCATTTCTAAAGGCT
  CGGCTGAACTGTTCTGTCCCTGGAGATTCGTTTTTCTACTTTGATGTTCTGCAGTCTATTACAGACATAA
  TACAAATCAATGGCATCCCCACTGTGGTCGGGGTGTTTACCACGCAGCTCAATAGCATCCCTGGTTCTGC
  TGTCTGTGCATTTAGCATGGATGACATTGAAAAAGTATTCAAAGGACGGTTTAAGGAACAGAAAACTCCA
  GATTCTGTTTGGACAGCAGTTCCCGAAGACAAAGTGCCAAAGCCAAGGCCTGGCTGTTGTGCAAAACACG
  GCCTTGCCGAAGCTTATAAAACCTCCATCGATTTCCCGGATGAAACTCTGTCATTCATCAAATCTCATCC
  CCTGATGGACTCTGCCGTTCCACCCATTGCCGATGAGCCCTGGTTCACAAAGACTCGGGTCAGGTACAGA
  CTGACGGCCATCTCAGTGGACCATTCAGCCGGACCCTACCAGAACTACACAGTCATCTTTGTTGGCTCTG
  AAGCTGGCATGGTACTTAAAGTTCTGGCAAAGACCAGTCCTTTCTCTTTGAACGACAGCGTATTACTGGA
  AGAGATTGAAGCCTACAACCATGCAAAGTGCAGTGCTGAGAATGAGGAAGACAAAAAGGTCATCTCATTA
  CAGTTGGATAAAGATCACCACGCTTTATATGTGGCGTTCTCTAGCTGCATTATCCGCATCCCCCTCAGTC
  GCTGTGAGCGTTATGGATCATGTAAAAAGTCTTGTATTGCATCTCGTGACCCGTATTGTGGCTGGTTAAG
  CCAGGGATCCTGTGGTAGAGTGACCCCAGGGATGCTTGCTGAAGGATATGAACAAGACACAGAATTCGGC
  AACACAGCTCATCTAGGGGACTGCCATGAAATTTTGCCTACTTCAACTACACCAGATTACAAAATATTTG
  GCGGTCCAACATCTGGTGTACGATGGGAAGTCCAGTCTGGAGAGTCCAACCAGATGGTCCACATGAATGT
  CCTCATCACCTGTGTCTTTGCTGCTTTTGTTTTGGGGGCATTCATTGCAGGTGTGGCAGTATACTGCTAT
  CGAGACATGTTTGTTCGGAAAAACAGAAAGATCCATAAAGATGCAGAGTCCGCCCAGTCATGCACAGACT
  CCAGTGGAAGTTTTGCCAAACTGAATGGTCTCTTTGACAGCCCTGTCAAGGAATACCAACAGAATATTGA
  TTCTCCTAAACTGTATAGTAACCTGCTAACCAGTCGGAAAGAGCTACCACCCAATGGAGATACTAAATCC
  ATGGTAATGGACCATCGAGGGCAACCTCCAGAGTTGGCTGCTCTTCCTACTCCTGAGTCTACACCCGTGC
  TTCACCAGAAGACCCTGCAGGCCATGAAGAGCCACTCAGAAAAGGCCCATGGCCATGGAGCTTCAAGGAA
  AGAAACCCCTCAGTTTTTTCCGTCTAGTCCGCCACCTCATTCCCCATTAAGTCATGGGCATATCCCCAGT
  GCCATTGTTCTTCCAAATGCTACCCATGACTACAACACGTCTTTCTCAAACTCCAATGCTCACAAAGCTG
  AAAAGAAGCTTCAAAACATTGATCACCCTCTCACAAAGTCATCCAGTAAGAGAGATCACCGGCGTTCTGT
  TGATTCCAGAAATACCCTCAATGATCTCCTGAAGCATCTGAATGACCCAAATAGTAACCCCAAAGCCATC
  ATGGGAGACATCCAGATGGCACACCAGAACTTAATGCTGGATCCCATGGGATCGATGTCTGAGGTCCCAC
  CTAAAGTCCCTAACCGGGAGGCATCGCTATACTCCCCTCCTTCAACTCTCCCCAGAAATAGCCCAACCAA
  GCGAGTGGATGTCCCCACCACTCCTGGAGTCCCAATGACTTCTCTGGAAAGACAAAGAGGTTATCACAAA
  AATTCCTCCCAGAGGCACTCTATATCTGCTATGCCTAAAAACTTAAACTCACCAAATGGTGTTTTGTTAT
  CCAGACAGCCTAGTATGAACCGTGGAGGATATATGCCCACCCCCACTGGGGCGAAGGTGGACTATATTCA
  GGGAACACCAGTGAGTGTTCATCTGCAGCCTTCCCTCTCCAGACAGAGCAGCTACACCAGTAATGGCACT
  CTTCCTAGGACGGGACTAAAGAGGACGCCGTCCTTAAAACCTGACGTGCCACCAAAGCCTTCCTTTGTTC
  CTCAAACCCCATCTGTCAGACCACTGAACAAATACACATACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153617 unedited
  TATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCCTGACTTCACCTTGGCCCA
  CCATGAGGGTCTTCCTGCTTTGTGCCTACATACTGCTGCTGATGGTTTCCCAGTTGAGGG
  CAGTCAGCTTTCCTGAAGATGATGAACCCCTTAATACTGTCGACTATCACTATTCAAGGC
  AATATCCGGTTTTTAGAGGACGCCCTTCAGGCAATGAATCGCAGCACAGGCTGGACTTTC
  AGCTGATGTTGAAAATTCGAGACACACTTTATATTGCTGGCAGGGATCAAGTTTATACAG
  TAAACTTAAATGAAATGCCCAAAACAGAAGTAATACCAAACAAGAAACTGACATGGCGAT
  CAAGACAACAGGATCGAGAAAACTGTGCTATGAAAGGCAAGCATAAAGATGAATGCCACA
  ACTTTATCAAAGTATTTGTTCCAAGAAACGATGAGATGGTTTTTGTTTGTGGTACCAATG
  CATTCAATCCCATGTGTAGATACTACAGGTTGAGTACCTTAGAATATGATGGGGAAGAAA
  TTAGTGGCCTGGCAAGATGCCCATTTGATGCCAGACAAACCAATGTTGCCCTCTTTGCTG
  ATGGGAAGCTGTATTCTGCCACAGTGGCTGACTTCTTGGCCAGCGATGCCCGTATTTATC
  GAAGCATGGGTGATGGATTCTGCCCTTTCGCACAATAAAATATGATTCCAAATGGATAAA
  AGAGCCCACACTTTTCTTCATGCCATAGAATATGGGANACTATGTCTATTTTTCTTCTTT
  CGAGAAAATCGCTGGTCGAACATTATAATNTAAGGCAGGGCTGGGTATTCTCCCGGGTGG
  CCCGCATATGTAAAACGACACTGGGTGGGTTCCACCCGGGTCTGGAGAACAACTGGACTT
  CATTCTTAAGGCTCGCCTGACCGTTCTGTCCTGAGAATACGTTCTTCTCTTTGAGAGAGG
  GCCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153617 unedited
  ACACATTGGCGATTGGCAACCTTCCAGGGNCCAGGGTGAGAGCACTGGAGGCAGGGGTAC
  ACAGGNGCATGCCACTCCGGGCATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATC
  TAGAGTCGAGTTTTTTTTTTTTTTTTTTAATGATTTGTATATTTTATTTTGACTTTACAT
  GCGGTGTTAAAAACCCAAAGTACATATTATTTACACATCCACAATACAAGTTTACAAGGT
  ATCTATTCATGTTAAAGTACTCTATAAAACAGTATAGAGACACTTCACTTGAGGGTTACT
  TTTTATGCCGTACCATTAAAAAAGATACAATCTGCGTTTACCTTTGCACAAATGGATAAA
  GCATTTAAAAATTAAATTAACAGGATAGAGACTAGATTTAATGTTAGAAATTTCAACATA
  AATACTGATTGAACTCACATTACAAACTACAAGGAAGCACAATGTTACAAAATGTTATCT
  GGGGTCACATAGAGTTACTGTTCTGTTTTACACACGCTGGGATTAATATTGGTAAGGTGA
  ATTCAAACACGTTTGTTGTTGGTGGACTGAGTAGTGTTTATCATTGGTTGGTTTAATTTA
  ATAATAAACTGGAATATTTTANTTGCCAAGCTGAAGTGTTTCAAGAACAGAAATTTTAGC
  TTTAGCGCTCCCAAAGATCACTGGAAAATCACGTTCTTAATAAAAAAATGTTTCTTAAAA
  AAAAAAAAAACTGGGATCAGNTTTCCCCATTACTGTAAAAAAATGATCTTGGTCCTCNGC
  AANATGTCCCCCCCCCAAATNNTAGGTCCCCACCGGATTAGCCAAAGAAAACCATTTGAA
  ACNCATTTACCTGATTACAGCNNNGGCAAAACCCNGGCGTGGGAATTTACAAGAATGGTT
  ACCCAAAAAAGTGAGTGGAGGACANNNAATTNACTGAAAAATGNNCAAAGTTCAAACAAT
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_153617
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153617.1, NP_705870.1
• RefSeq Size: 5941 bp
• RefSeq ORF: 3054 bp
• Locus ID: 80031
• UniProt ID: Q8NFY4
• Cytogenetics: 15q21.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (3) lacks an internal exon, compared to variant 4. The encoded isoform (3) is missing an internal segment relative to isoform 4.