PDE1C (NM_005020) Human Untagged Clone

CAT#: SC124021

PDE1C (untagged)-Human phosphodiesterase 1C, calmodulin-dependent 70kDa (PDE1C), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE1C
• Synonyms: cam-PDE 1C; DFNA74; hCam-3; Hcam3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124021 sequence for NM_005020 edited (data generated by NextGen Sequencing)
  ATGGAGTCGCCAACCAAGGAGATTGAAGAATTTGAGAGCAACTCTCTGAAATACCTGTAA
  CCGGAACAGATCGAGAAAATCTGGCTTCGGCTCCGCGGGCTGAGGAAATATAAGAAAACG
  TCCCAGAGATTACGGTCTTTGGTCAAACAATTAGAGAGAGGGGAAGCTTCAGTGGTAGAT
  CTTAAGAAGAATTTGGAATATGCAGCCACAGTGCTTGAATCTGTGTATATTGATGAAACA
  AGGAGACTCCTGGATACAGAGGATGAGCTCAGTGACATTCAGTCAGATGCTGTGCCTTCT
  GAGGTCCGAGACTGGCTGGCCTCCACCTTCACGCGGCAGATGGGGATGATGCTCAGGAGG
  AGCGACGAGAAGCCCCGGTTCAAGAGCATCGTTCACGCAGTGCAGGCTGGGATATTTGTG
  GAGAGAATGTATAGACGGACATCAAACATGGTTGGACTGAGCTATCCACCAGCTGTTATT
  GAGGCATTAAAGGATGTGGACAAGTGGTCCTTTGACGTCTTTTCCCTCAATGAGGCCAGT
  GGGGATCATGCACTGAAATTTATTTTCTATGAACTACTCACACGTTATGATCTGATCAGC
  CGTTTCAAGATCCCCATTTCTGCACTTGTCTCATTTGTGGAGGCCCTGGAAGTGGGATAC
  AGCAAGCACAAAAATCCTTACCATAACTTAATGCACGCTGCCGATGTTACACAGACAGTG
  CATTACCTCCTCTATAAGACAGGAGTGGCGAACTGGCTGACGGAGCTGGAGATCTTTGCT
  ATAATCTTCTCAGCTGCCATCCATGACTACGAGCATACCGGAACCACCAACAATTTCCAC
  ATTCAGACTCGGTCTGATCCAGCTATTCTGTATAATGACAGATCTGTACTGGAGAATCAC
  CATTTAAGTGCAGCTTATCGCCTTCTGCAAGATGACGAGGAAATGAATATTTTGATTAAC
  CTCTCAAAGGATGACTGGAGGGAGTTTCGAACCTTGGTAATTGAAATGGTGATGGCCACA
  GATATGTCTTGTCACTTCCAACAAATCAAAGCAATGAAGACTGCTCTGCAGCAGCCAGAA
  GCCATTGAAAAGCCAAAAGCCTTATCCCTTATGCTGCATACAGCAGATATTAGCCATCCA
  GCAAAAGCATGGGACCTCCATCATCGCTGGACAATGTCACTCCTGGAGGAGTTCTTCAGA
  CAGGGTGACAGAGAAGCAGAGCTGGGGCTGCCTTTTTCTCCTCTGTGTGACCGAAAGTCC
  ACTATGGTTGCTCAGTCACAAGTAGGTTTCATTGATTTCATCGTGGAACCCACCTTCACT
  GTGCTTACGGACATGACCGAGAAGATTGTGAGTCCATTAATCGATGAAACCTCTCAAACT
  GGTGGGACAGGACAGAGGCGTTCGAGTTTGAATAGCATCAGCTCGTCAGATGCCAAGCGA
  TCAGGTGTCAAGACCTCTGGTTCAGAGGGAAGTGCCCCGATCAACAATTCTGTCATCTCC
  GTTGACTATAAGAGCTTTAAAGCTACTTGGACGGAAGTGGTGCACATCAATCGGGAGAGA
  TGGAGGGCCAAGGTACCCAAAGAGGAGAAGGCCAAGAAGGAAGCAGAGGAAAAGGCTCGC
  CTGGCCGCAGAGGAGCAGCAAAAGGAAATGGAAGCCAAAAGCCAGGCTGAAGAAGGCGCA
  TCTGGCAAAGCTGAGAAAAAGACGTCTGGAGAAACTAAGAATCAAGTCAATGGAACACGG
  GCAAACAAAAGTGACAACCCTCGTGGGAAAAATTCCAAAGCTGAGAAGTCATCAGGAGAA
  CAGCAACAGAATGGTGACTTCAAAGATGGCAAAAATAAGACAGACAAGAAGGATCACTCT
  AACATCGGAAATGATTCAAAGAAAACAGATGATTCACAAGAGTAA
  
  Clone variation with respect to NM_005020.2
  58 c=>t;1773 c=>t;1782 c=>t;1830 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005020 unedited
  TTTGAATACGACTGACTATAGGGCGGCCGCGAATTCGGCACGAGGGACAGCCCAAACTAT
  CTCAGCTTCACCGCCAGTGGTCACCTACCTGCAGCTATGGAGTCGCCAACCAAGGAGATT
  GAAGAATTTGAGAGCAACTCTCTGAAATACCTGTAACCGGAACAGATCGAGAAAATCTGG
  CTTCGGCTCCGCGGGCTGAGGAAATATAAGAAAACGTCCCAGAGATTACGGTCTTTGGTC
  AAACAATTAGAGAGAGGGGAAGCTTCAGTGGTAGATCTTAAGAAGAATTTGGAATATGCA
  GCCACAGTGCTTGAATCTGTGTATATTGATGAAACAAGGAGACTCCTGGATACAGAGGAT
  GAGCTCAGTGACATTCAGTCAGATGCTGTGCCTTCTGAGGTCCGAGACTGGCTGGCCTCC
  ACCTTCACGCGGCAGATGGGGATGATGCTCAGGAGGAGCGACGAGAAGCCCCGGTTCAAG
  AGCATCGTTCACGCAGTGCAGGCTGGGATATTTGTGGAGAGAATGTATAGACGGACATCA
  AACATGGTTGGACTGAGCTATCCACCAGCTGTTATTGAGGCATTAAAGGATGTGGACAAG
  TGGTCCTTTGACGTCTTTTCCCTCAATGAGGCCAGTGGGGATCATGCACTGAAATTTATT
  TTCTATGAACTACTCACACGTTATGATCTGATCAGCCGTTTCAAGATCCCCATTTCTGCA
  CTTGTCTCATTTGTGGAGGCCCTGGAAGT
  
• Restriction Sites: Please inquire
• ACCN: NM_005020
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005020.1, NP_005011.1
• RefSeq Size: 2694 bp
• RefSeq ORF: 1905 bp
• Locus ID: 5137
• UniProt ID: Q14123
• Cytogenetics: 7p14.3
• Domains: PDEase, HDc
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Olfactory transduction, Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: This gene encodes an enzyme that belongs to the 3'5'-cyclic nucleotide phosphodiesterase family. Members of this family catalyze hydrolysis of the cyclic nucleotides, cyclic adenosine monophosphate and cyclic guanosine monophosphate, to the corresponding nucleoside 5'-monophosphates. The enzyme encoded by this gene regulates proliferation and migration of vascular smooth muscle cells, and neointimal hyperplasia. This enzyme also plays a role in pathological vascular remodeling by regulating the stability of growth factor receptors, such as PDGF-receptor-beta. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (4) differs in the 5' and 3' UTR and has multiple coding region differences, compared to variant 3. These differences result in an isoform (1) with distinct N- and C-termini, compared to isoform 3. Variants 1 and 4 encode the same protein. Isoform 1 is also known as HCam-3B. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.