ACCN1 (ASIC2) (NM_001094) Human Untagged Clone

CAT#: SC124011

ASIC2 (untagged)-Human amiloride-sensitive cation channel 1, neuronal (ACCN1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACCN1
• Synonyms: ACCN; ACCN1; ASIC2a; BNaC1; BNC1; hBNaC1; MDEG
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001094, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACCTCAAGGAAAGCCCCAGTGAGGGCAGCCTGCAACCTTCTAGCATCCAGATCTTTGCCAACACCT
  CCACCCTCCATGGCATCCGCCACATCTTCGTGTATGGGCCGCTGACCATCCGGCGTGTGCTGTGGGCAGT
  GGCCTTCGTGGGCTCTCTGGGCCTGCTGCTGGTGGAGAGCTCTGAGAGGGTGTCCTACTACTTCTCCTAC
  CAGCATGTCACTAAGGTGGACGAAGTGGTGGCTCAAAGCCTGGTCTTCCCAGCTGTGACCCTCTGTAACC
  TGAATGGCTTCCGGTTCTCCAGGCTCACCACCAATGACCTGTACCATGCTGGGGAGCTGCTGGCCCTGCT
  GGATGTCAACCTGCAGATCCCGGACCCCCATCTGGCTGACCCCTCCGTGCTGGAGGCCCTGCGGCAGAAG
  GCCAACTTCAAGCACTACAAACCCAAGCAGTTCAGCATGCTGGAGTTCCTGCACCGTGTGGGCCATGACC
  TGAAGGATATGATGCTCTACTGCAAGTTCAAAGGGCAGGAGTGCGGCCACCAAGACTTCACCACAGTGTT
  TACAAAATATGGGAAGTGTTACATGTTTAACTCAGGCGAGGATGGCAAACCTCTGCTCACCACGGTCAAG
  GGGGGGACAGGCAACGGGCTGGAGATCATGCTGGACATTCAGCAGGATGAGTACCTGCCCATCTGGGGAG
  AGACAGAGGAAACGACATTTGAAGCAGGAGTGAAAGTTCAGATCCACAGTCAGTCTGAGCCACCTTTCAT
  CCAAGAGCTGGGCTTTGGGGTGGCTCCAGGGTTCCAGACCTTTGTGGCCACACAGGAGCAGAGGCTCACA
  TACCTGCCCCCACCGTGGGGTGAGTGCCGATCCTCAGAGATGGGCCTCGACTTTTTTCCTGTTTACAGCA
  TCACCGCCTGTAGGATTGACTGTGAGACCCGCTACATTGTGGAAAACTGCAACTGCCGCATGGTTCACAT
  GCCAGGGGATGCCCCTTTTTGTACCCCTGAGCAGCACAAGGAGTGTGCAGAGCCTGCCCTAGGTCTGTTG
  GCGGAAAAGGACAGCAATTACTGTCTCTGCAGGACACCCTGCAACCTAACCCGCTACAACAAAGAGCTCT
  CCATGGTGAAGATCCCCAGCAAGACATCAGCCAAGTACCTTGAGAAGAAATTTAACAAATCAGAAAAATA
  TATCTCAGAGAACATCCTTGTTCTGGATATATTTTTTGAAGCTCTCAATTATGAGACAATTGAACAGAAG
  AAGGCGTATGAAGTTGCTGCCTTACTTGGTGATATTGGTGGTCAGATGGGATTGTTCATTGGTGCTAGTA
  TCCTTACAATACTAGAGCTCTTTGATTATATTTATGAGCTGATCAAAGAGAAGCTATTAGACCTGCTTGG
  CAAAGAGGAGGACGAAGGGAGCCACGATGAGAATGTGAGTACTTGTGACACAATGCCAAACCACTCTGAA
  ACCATCAGTCACACTGTGAACGTGCCCCTGCAGACGACCCTGGGGACCTTGGAGGAGATTGCCTGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001094 unedited
  GGTTAGCGGTCACTATAGGGNCGGCCGCGCNNNNNNNGGAGCATCACCCTGATGTCTCCC
  CAAGAAAAACCAGCCGTGCTTATCGCCTGGAAAAAATCAAGAGCTAGAGGGTGAATGGGT
  TAGGGGTAAACGGGGACCCCTGTCAACGTGATAAAGGCTCTCAGGATAAACTCACAGCAT
  CTGGCGCGATGCTTGCCTTGCGTTCTCTCCCCTGAACGTCAAGGTTTAAGCAGAGCCCGA
  GGACTGGGAGCTCTTCTCTGAAATTCGATCAACCTGAAGCCAGTTGCGGAACTGCACGGG
  GTCCCGATGGACCTCAAGGAAAGCCCCAATGAGGGCAGCCTGCAACCTTCTAGCATCCAG
  ATCTTTGCCAACACCTCCACCCTCCATGGCATCCGCCACATCTTCGTGTATGGGCCGCTG
  ACCATCCGGCGTGTGCTGTGGGCAATGGCCTTCGTGGGCTCTCTGGGCCTGCTGCTGGTG
  GAGAGCTCTGAGAGGGTGTCCTACTACTTCTCCTACCAGCATGTCACTAAGGTGGACGAA
  GTGGTGGCTCAAAACCCGGTCTTCCCAGCTGTGACCCTCTGGAACCTGAATGGCTTCCGG
  GTCTCCAGGCTCACCACCCAAAGACCTGTACCATGCTGGGGAGCTGCTGGCCCTGCTGAT
  GTCAACCTGCAGATCCCGGACCCCCATCTGGCTGACCCCTCCCTGCTGGAAGCCCTGCGG
  CAGAAAGCCACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001094 unedited
  CTTCTCTCGTGGCTCCCTTCGTCCTAGGTNNGNGAAGCAGGTCTAATAGCTTCTCTTTGA
  TCAGCTCATAAATATTTTCAAAGAGCTCTAGTATTGTAAGGATACTAGCGCCAATGAACA
  ATCCCATCTGACCACCAATATCACCAATAATAGCAGCAACTTCATACGCCTTCTTCTGTT
  CAATTGTCTCATAATTGAGAGCTTCAAAAAATATATCCAGAACAAGGATGTTCTCTGAGA
  TATATTTTTCTGATTTGTTAAATTTCTTCTCAAGGTACTTGGCTGATGTCTTGCTGGGGA
  TCTTCACCATGGAGAGCTCTTTGTTGTAACGGGTTAAGTTGCAAGGTGTCCTGCAGAGAC
  AGTAATTGCTGTCCTTTTCCGCCAACAGACCTAGGGCAGGCTCTGCACACTCCTTGTGCT
  GCTCAGGGGTACAAAANAGGGCATCCCCTGGCATGTGAACCATGCGGCAGTTGCAGTTTT
  CCACAATGTAGCGGGTCTCACAGTCAATCCTACAGGCGGTGATGCTGTAAACAGGAAAAA
  AGTCCAGGCCCATCTCTGAGGATCGGCACTCACCCCACGGGGGGGGCAAGGTATGTAAGC
  CCTCTGCTCCCTGTGTGGCCACAAAGGTCTGGAACCCTGGAACCCACCCCAAACCCAACT
  CTTGGATGAAAGGTGGCTAAGACTGACTGTGGATCTGAACTTTAACTCCTGCTTCAAATG
  TCGTTTCCTCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001094
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001094.4, NP_001085.2
• RefSeq Size: 2747 bp
• RefSeq ORF: 1539 bp
• Locus ID: 40
• UniProt ID: Q16515
• Cytogenetics: 17q11.2-q12
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Taste transduction
• Gene Summary: This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (MDEG1) uses an alternate 5'-terminal exon, compared to variant MDEG2, resulting in a shorter isoform (MDEG1) with a distinct N-terminus, compared to isoform MDEG2.