Kv1.8 (KCNA10) (NM_005549) Human Untagged Clone

CAT#: SC124007

KCNA10 (untagged)-Human potassium voltage-gated channel, shaker-related subfamily, member 10 (KCNA10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Kv1.8
• Synonyms: Kcn1; Kv1.8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005549 edited
  GGCACCAGGTGAAGGGAGCATAAGTAACTTGTTCTCTGTCACGCAGACACTAAGTGGCCA
  AGCCAGGATTGGGAGCTGGCCCTCCTGTCTGCCCCCAGAGCCTGTGCTCCTGACCAGGAT
  GGAGAGCCTGCCTCAGGCTGGATGAGGGGCAGCCCCCACACCCAGCCTGCTGACCTCCCC
  ACTGAATTCAGGTCTCCTGACGGACGCTGCTCTCAGTTTCCAGCTCTACCCTTTCCATGG
  CCCTAAGGCTGACATCGCCACCTCTGCTTTCCACTCAGAAAGGCTGGAGGAGCTGGGCAC
  TCGAGGGACCAGAGGGGCTACAGTGAGCAGCAGAGCCAGCGAGTCTTGCCTAAGACCCCA
  TCTTACCATTCCCTGGTTGGGGAAACTGAGGTTCTTGGAGAGTGAGGCAACATAATCCCG
  ACCACGTGAGACAGAGAGGTACAGAATCCACACTCTTCCGGCCGGGCCGTGGTTCAGCAT
  GAGCCGAAGAGATGGGGCTTGGCTCTAAGAGGGGCAGAGACCAGAAAGGGTGAGGTTTGA
  GAGCCACTCACTGGGGAGAATCTGCAAGACCTGAGGCCTGGCCGACCCTAAAGTGAAAGA
  AAAGCGGCATCAAAGAGCTCACAGCAGGCAGTGAAGGCTGAGGATCTTCATGCTTCTTCC
  CTGGCTCCCCTAGAATGGATGTGTGTGGCTGGAAAGAAATGGAGGTTGCGCTGGTCAATT
  TTGATAATTCAGATGAAATCCAAGAAGAGCCAGGCTATGCCACAGACTTCGACTCAACCA
  GCCCAAAAGGCCGGCCTGGGGGCAGCTCCTTCTCCAACGGGAAGATCCTCATCAGCGAAA
  GCACCAACCATGAGACGGCCTTCTCCAAGCTTCCGGGAGACTATGCTGACCCCCCAGGGC
  CTGAGCCAGTGGTCCTAAATGAAGGAAACCAGCGGGTGATCATCAACATTGCTGGGCTGA
  GATTTGAGACCCAGCTCAGAACCCTTAGTCAGTTCCCAGAGACTCTCCTGGGAGACCGGG
  AAAAAAGGATGCAGTTCTTTGACTCCATGAGAAATGAGTATTTCTTTGATCGGAACCGGC
  CCAGTTTTGATGGAATCCTATATTATTACCAATCTGGTGGGAAAATTCGGCGCCCAGCCA
  ATGTTCCCATTGATATCTTTGCTGATGAAATCTCCTTCTATGAGCTGGGTAGTGAGGCCA
  TGGACCAGTTCCGGGAGGATGAAGGCTTCATCAAAGACCCTGAAACACTGCTACCCACCA
  ATGACATCCACCGTCAGTTCTGGCTCCTCTTTGAGTACCCTGAAAGTTCCAGCGCTGCCC
  GTGCTGTGGCCGTGGTCTCGGTGTTGGTTGTGGTCATCTCCATCACCATCTTCTGCCTGG
  AGACACTGCCAGAGTTCCGGGAGGATAGGGAGCTAAAGGTGGTCAGAGACCCCAATCTCA
  ACATGAGCAAGACAGTCCTCTCCCAGACCATGTTCACCGACCCTTTCTTCATGGTGGAGT
  CTACCTGCATCGTGTGGTTCACCTTCGAGCTGGTGCTCCGGTTCGTGGTCTGCCCCAGCA
  AGACTGACTTCTTCAGGAACATCATGAACATCATTGACATCATCTCCATTATCCCCTACT
  TTGCAACTCTCATCACAGAGCTAGTCCAGGAGACAGAGCCGAGTGCCCAACAGAACATGT
  CCCTGGCCATCCTGAGGATCATCCGCCTGGTGAGGGTCTTCCGCATCTTCAAGCTCTCGC
  GCCACTCCAAGGGGCTGCAGATCCTCGGGCAAACACTGAAGGCGTCCATGCGGGAGTTGG
  GGTTGCTCATCTTCTTTCTCTTCATTGGAGTCATCCTCTTCTCCAGTGCAGTCTACTTTG
  CTGAGGTGGATGAGCCAGAGTCCCATTTCTCTAGCATTCCTGATGGCTTCTGGTGGGCAG
  TGGTCACCATGACAACTGTAGGCTATGGGGACATGTGCCCGACCACCCCAGGGGGGAAGA
  TTGTGGGCACTCTGTGTGCCATTGCAGGGGTCCTCACCATTGCCCTCCCTGTGCCTGTCA
  TTGTCTCCAACTTCAATTACTTCTACCACCGGGAGACTGAGAATGAAGAAAAGCAGAACA
  TCCCAGGAGAAATTGAAAGAATCCTCAACAGTGTAGGCTCAAGAATGGGCAGCACAGACT
  CTCTTAATAAGACCAATGGTGGCTGTTCCACAGAGAAGTCTAGGAAATGATCAGTCCAGG
  GCTTCTTGGGTCCATCCTGTCTCTCTTCTCTCTTCTCTCTCTCTCTCTCTCGCTTTCTCC
  CCTCCTCTCTCTCTTCCTTCTGCCTTCCCTTCCTCCCTTCATTCCTTCCTCTTTCCCTCT
  CCCTTCTCTCATTTCATCTTTCCAAAGATCAAAATGTATCTTTTGTGATCAATAACTATC
  TTTGACTACAAGCTATTTTAATAAGCCCATACTTCTACTGTATTTTCTAAACTGTTTCTT
  TTCAATACATTATAGAAAGATTTGGATCTCTTTTCCTATGAAGGGATAGTTCAGGTCCCC
  AGACCAGAAATACTGGTATCACCCGGGAGGATGAAAATGCAGAATC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005549 unedited
  GTGNATAGGNAGCATAATTCACTTGTCCTTTGTCACGCAGGCACTACCTGGCCAATCCAG
  GATGGCAGCTGGCATCTCCTGTCTGCCCCCAGAGCCTGTGCTCCTGACCAGGTAGGCAGA
  GCCTGGACTCAGCTGGATGAGGGGCAGCCCCCGCACCCAGCCTGCTGACCTCCCCACTGA
  ATTCAGGTCTCCTGACGGACGCTGCTCTTAGTTTCCAGCTCTACCCTTTCCATGCCCCTA
  ATGCTGCAATCGCCACCTCTGTTTTCCACTCACAAACGCTGGTAGAGCTGGGCACTCGAG
  GGACCATTGCCCCTACCGTGATCTTTATAGCCAGCGAGTCTTGCCTAACACCCCATGCTT
  ACCCTTCCCTGGTTGGGGAAACTCGAGTCCTTGGACATTGAGGCCTCCATATTTCGACCG
  CGGTGATATTATCCGTTCAGAATCCACCCGCTTTCGGCCGTGTCCGCGTTTTACTCTCGA
  CCCCAATAGATGGCTCTCGGCCCCTTACACGGGCCTAGCCCCAAGCGGGTGCCGCTTTTA
  GAACCCCATCCCTCGCCGACCACCTGCCGGTACCTCATGTCCTGTCCCTTTCTCTTTGAT
  GCCAGAAAAACGCGCATATAACCGCCTCACGCGTATGACGTGTGAGGCACGAGGATTTGT
  ATGGCCTCTAGCCGTGTGTGCCCGACTACCGGTTGTCGTGCTGTTTGCCCACGACACTCC
  GGCTGCTCCCTGCTTTTCTTTATCACCTTTTTACCATTAATCGGCTCCGACGGCCCCGGT
  CGAGTGGTATTGGAACGGTGGGATGGTCTTAATCTCCCCGAGGTTTCGCCCGGGTGTGTC
  TTTTAATTATTATCACGGCGGACCATCCACTCTGATGCCCACATTTATCTTTTTTGCCCC
  ACACCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005549 unedited
  TTCATTTCTCCTGAGATGTTCTGCTTTATTTCATTCTCAGTCTCCCGGTGGTAGAAGTAA
  TTGAAGTTGGAGACAATGACAGGCACAGGGAGGGCAATGGTGAGGACCCCTGCAATGGCA
  CACAGAGTGCCCACAATCTTCCCCCCTGGGGTGGTCGGGCACATGTCCCCATAGCCTACA
  GTTGTCATGGTGACCACTGCCCACCAGAAGCCATCAGGAATGCTAGAGAAATGGGACTCT
  GGCTCATCCACCTCAGCAAAGTAGACTGCACTGGAGAAGAGGATGACTCCAATGAAGAGA
  AAGAAGATGAGCAACCCCAACTCCCGCATGGACGCCTTCAGTGTTTGCCCGAGGATCTGC
  AGCCCCTTGGAGTGGCGCGAGAGCTTGAAGATGCGGAAGACCCTCACCAGGCGGATGATC
  CTCAGGATGGCCAGGGACATGTTCTGTTGGGCACTCGGCTCTGTCTCCTGGACTAGCTCT
  GTGATGAGAGTTGCAAAGTAGGGGATAATGGAGATGATGTCAATGATGTTCATGATGTTC
  CTGAAGAAGTCAGTCTTGCTGGGGCAGACCACGAACCGGAGCACCAGCTCGAAGGTGAAC
  CACACGATGCAGGTAGACTCCACCATGAAGAAAGGGTCGGTGAACATGGTCTGGGAGAGG
  ACTGTCTTGCTCATGTTGAGATTGGGGTCTCTGACCACCTTTAGCTCCCTATCCNTCCGG
  AACTCTGGCAGTGTN
  
• Restriction Sites: Please inquire
• ACCN: NM_005549
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: no
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005549.2, NP_005540.1
• RefSeq Size: 1959 bp
• RefSeq ORF: 1536 bp
• Locus ID: 3744
• UniProt ID: Q16322
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It is specifically regulated by cGMP and postulated to mediate the effects of substances that increase intracellular cGMP. This gene is intronless, and the gene is clustered with genes KCNA2 and KCNA3 on chromosome 1. [provided by RefSeq, Jul 2008]