IGF1 (BC012881) Human Untagged Clone

CAT#: SC123649

(untagged)-Human cDNA clone MGC:17553 IMAGE:3463076, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IGF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC012881 edited
  GAGCCACCTCCCTGAGGCCGCTGCATCGCTTCCTTACAGCAGTCAACCACAGACTTTAAT
  TAATACAATAAGGTACAGACAACAGGGTGCCACGGACATTTAGGCCCAAGGAATATTATG
  CAAAAGCAGTGTTTTAAGGGAGTTTCACGTGACAGACCAGCAGATTACACTGAACAAAGA
  AACAAGAAGTGATGTTGTCCCTGCCTGGAAGTGAACTTGGGGAGGAGAGAGGGAGAATTA
  TTCACAAAATCTCCTTCCCCAAACAGTCAACACCCAGAACCAACATGGAGCTCCTACCCC
  ACTGGGACCTGCTGACTGAATTCTTTCTGAGACCCAGTAAGGAGAACTGGTTCCGGCAGC
  TGAGAAGCCAGGCTGTCTGCTAACGGCCTACAAGGGGCTCTCTCGCTGCTGTTAGGGCTG
  GTCTGCTCTGTGTCCGTGGTCCAGTTGGGTACAACAAACCCATTCACTCAACAGTGCCAG
  AGACCCCTCTGGAGACTGGGGAAGCAGCTTCCTTGTGGTCGAGAGCTGTGCAGCCGGGAC
  AGGAGCCTGCATTCGTTATCACACATCACGGCCTTCGGCTTGGCTGGGGGCCAGGTGGGC
  ATGGATGCTGGCATGCCCACAGGCAGATCTCAGCTTTCCAGTGAGGTAGCAGCCTGCCGC
  ACCAAGACTCAGGGCTGTCATGAGGAAGAGCCCCTGGGACCCCCAGCATCCTCGCGAACT
  CCCTGGAGCTTCATCATGGCCCATGCGCAGCACACGGCAGACAACCGGTTCAGCAGTCCC
  TGGAGCCCACGTGCCTCCCCTTCCTTTTGCCTTGGCTCCCTTATGCCCACCCACCAGCTC
  TTCCAGGCTCCAAAGGGGAAGTTGAAGAAGAATCTGGTACCTGGCCCAGCTGCCAAGCCC
  CAAGGTCACACAGCCCTAGAACCCTGCTGCCAAGTCCGTCTCGGGGGACCTAGGAGAGGA
  AGTCCGCCAGCCTGGGAAGCTGCCTCTCCAGCCTTGGCCAAGCACCACCTGGCAAACTGC
  AGCTGAAATGCCGAGCACACTACAGGGGGATACCCCCTCCACCCCAGCCCTGCTGAGAAG
  CCCAGCCACACTCTTGATCTCCCTGAAATCCTTGAATCTAGGTCAAGGGTGGTGCTAGGG
  GTTGGATGCACAGGGAAGAGGCTGAGCACCAAGACATGTGGACCAGTCAGGACTAGAGAC
  CTCCTTCCTTCCAGACATGCACACTCAGCCACGCTGGAGATGACAAAACCTTACACACTC
  AAAGCCAATACATGCGTAGGGGCGGCCACATGCCACGCACTGTTCTAAATATCTTGTACA
  AACTCAGCTAATCCTCAGGACAATTCTCATTTTACAGATGAGGAGACCGAGGCACAGCGT
  GGTGAAGTGACTTGTTCATGGTCACATAGGTGGTAAGGCAGTGAGGTGGAACTTGAACCC
  AGACAAGCAGCCTCCACTCCCCCCACCTGCTCACCTCTAACTCAGGCCGCTCTTCGAAGA
  CAACCTGAAGATTTTTCTTTGCAATCGATAGTCTAGAGCAGGAGTCTCCAAGGGGAGAGA
  CCATGTCCCACTGGGAGCCCAAAGCACTCCAGGGAGGGTAGGAAAAAAATATGAGAGCTT
  TTGTACTGATTTTGTTTCCTCACTCCTGTTTCCTTCTCTCCCCTTGTGGGTGAGGGCGAG
  TGAGCAGTTGATCATCTCTTAAACCAGGGCTGCCAGAGCCAGAGCCAGAGCCAGAAAATG
  GAGTGCCTTCCTTCCTTCCTTTCCTTCCTTCCTTTCCTTCCTTCCTTCCTCCCTCCCTCT
  CTTTTTTTTTGATGGAGTCTAGCTCTGTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC012881 unedited
  AACCTAAGATTTGTATACGACTTTTTATAGGCGGCCGCGCATTCTTTCTGAAACCCAGTA
  AGGAGAACTGGTTCCGGCAGCTGAGAAGCCAGGCTGTCTGCTAACGGCCTACAAGGGGCT
  CTCTCGCTGCTGTTAGGGCTGGTCTGCTCTGTGTCCGTGGTCCAGTTGGGTACAACAAAC
  CCATTCACTCAACAGTGCCAGAGACCCCTCTGGAGACTGGGGAAGCAGCTTCCTTGTGGT
  CGAGAGCTGTGCAGCCGGGACAGGAGCCTGCATTCGTTATCACACATCACGGCCTTCGGC
  TTGGCTGGGGGCCAGGTGGGCATGGATGCTGGCATGCCCACAGGCAGATCTCAGCTTTCC
  AGTGAGGTAGCAGCCTGCCGCACCAAGACTCAGGGCTGTCATGAGGAAGAGCCCCTGGGA
  CCCCCAGCATCCTCGCGAACTCCCTGGAGCTTCATCATGGCCCATGCGCAGCACACGGCA
  GACAACCGGTTCAGCAGTCCCTGGAGCCCACGTGCCTCCCCTTCCTTTTGCCTTGGCTCC
  CTTATGCCCACCCACCAGCTCTTCCAGGCTCCAAAGGGGAAGTTGAAGAAGAATCTGGTA
  CCTGGCCCAGCTGCCAAGCCCCAAAGTCACACAGCCCTAGAACCCTGCTGCCAAGTCCGT
  CTCGGGGGACCTAGGAGAGGAAGTCCGCCAGCCTGAGAAGCTGCCTCTCCAGCCTTGGCC
  AAGCACCACCTGGCATACTGCAGCTGATATGCCGAGCACACTACAGGGGGGATACCCCCT
  CCACCCCCAGCCCTGCTGAGAAGCCCAACCACACTCTTGATCTCCCTGAAATCCCTGAAT
  CTAGGTCAAGGGTGGGTGCTAGGGGTTGGATGCACAGGGAAAAGCTGAGCACCAGACTGG
  GGGCCAGTCAGGACAGAGAACCTCT
  
• Restriction Sites: Please inquire
• ACCN: BC012881
• Insert Size: 1832 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012881.1
• RefSeq Size: 1832 bp
• Locus ID: 3479
• Cytogenetics: 12q23.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Dilated cardiomyopathy, Focal adhesion, Glioma, Hypertrophic cardiomyopathy (HCM), Long-term depression, Melanoma, mTOR signaling pathway, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer
• Gene Summary: The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]