TIA1 (BC015944) Human Untagged Clone

CAT#: SC123619

TIA1 (untagged)-Human TIA1 cytotoxic granule-associated RNA binding protein (cDNA clone MGC:9449 IMAGE:3909177), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TIA1
• Synonyms: TIA-1; WDM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC015944 edited
  GGCGCCGCCGCGACAGCAGCAGCCATGGAGGACGAGATGCCCAAGACTCTATACGTCGGT
  AACCTTTCCAGAGATGTGACAGAAGCTCTAATTCTGCAACTCTTTAGCCAGATTGGACCT
  TGTAAAAACTGCAAAATGATTATGGATACAGCTGGAAATGATCCCTATTGTTTTGTGGAG
  TTTCATGAGCATCGTCATGCAGCTGCAGCATTAGCTGCTATGAATGGACGGAAGATAATG
  GGTAAGGAAGTCAAAGTGAATTGGGCAACAACCCCTAGCAGTCAAAAGAAAGATACAAGC
  AGTAGTACCGTTGTCAGCACACAGCGTTCACAAGATCATTTCCATGTCTTTGTTGGTGAT
  CTCAGCCCAGAAATTACAACTGAAGATATAAAAGCTGCTTTTGCACCATTTGGAAGAATA
  TCAGATGCCCGAGTGGTAAAAGACATGGCAACAGGAAAGTCTAAGGGATATGGCTTTGTC
  TCCTTTTTCAACAAATGGGATGCTGAAAACGCCATTCAACAGATGGGTGGCCAGTGGCTT
  GGTGGAAGACAAATCAGAACTAACTGGGCAACCCGAAAGCCTCCCGCTCCAAAGAGTACA
  TATGAGTGTAGGTGTATTGGAGAAGAAAAGGAAATGTGGAATTTTGGAGAAAAATACGCT
  AGATTTTAAATGTTAGAGCTGTTCCCGGAGACTTATTGCAGAAATAGATGAGAAGCAAAT
  CAAGACTACTATTCAAAAATGTACTTAGTTTTCATTTTTGTAATTATAAATAATATTATT
  TCTAATGTCAAGTCTCCTATTAAATAGAAAATACTGGGTAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC015944 unedited
  NNGGTCAGAATTTGTATACGACTCATATAGGCGGCCGCGNAATCCCGGGATATCGTCGAC
  CCACGCGTCCGGGCGCCGCCGCGACAGCAGCAGCCATGGAGGACGAGATGCCCAAGACTC
  TATACGTCGGTAACCTTTCCAGAGATGTGACAGAAGCTCTAATTCTGCAACTCTTTAGCC
  AGATTGGACCTTGTAAAAACTGCAAAATGATTATGGATACAGCTGGAAATGATCCCTATT
  GTTTTGTGGAGTTTCATGAGCATCGTCATGCAGCTGCAGCATTAGCTGCTATGAATGGAC
  GGAAGATAATGGGTAAGGAAGTCAAAGTGAATTGGGCAACAACCCCTAGCAGTCAAAAGA
  AAGATACAAGCAGTAGTACCGTTGTCAGCACACAGCGTTCACAAGATCATTTCCATGTCT
  TTGTTGGTGATCTCAGCCCAGAAATTACAACTGAAGATATAAAAGCTGCTTTTGCACCAT
  TTGGAAGAATATCAGATGCCCGAGTGGTAAAAGACATGGCAACAGGAAAGTCTAAGGGAT
  ATGGCTTTGTCTCCTTTTTCAACAAATGGGATGCTGAAAACGCCATTCAACAGATGGGTG
  GCCAGTGGCTTGGTGGAAGACAAATCAGAACTAACTGGGCAACCCGAAAGCCTCCCGCTC
  CAAAGAGTACATATGAGTGTAGGTGTATTGGGAGAAGAAAAGGAAATGTGGAATTTTGGG
  AGAAAAATACGCTAGATTTTAAATGTTAGAGCTGTTCCCGGAGACTTATTGCAGAAATAG
  ATGAGAAGCANATCAAGACTACTATTCAAAAATGTACTTAGTTNTCATTTTTGTAATTAT
  AAATAATATTATTTCTAATGTCAAGTCTCCTATTAAATAGAAAATACTGGGTAAAAAAAA
  AAAAAGGGCNGCCGCNGTCATAGCTGTTTCTGAACGATCCCG
  
• Restriction Sites: Please inquire
• ACCN: BC015944
• Insert Size: 834 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015944.1, AAH15944.1
• RefSeq Size: 834 bp
• Locus ID: 7072
• Cytogenetics: 2p13.3
• Protein Families: Druggable Genome
• Gene Summary: The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms has been found for this gene. [provided by RefSeq, May 2017]