IGHM (BC017356) Human Untagged Clone

CAT#: SC123605

(untagged)-Human immunoglobulin heavy constant mu (cDNA clone MGC:29722 IMAGE:5095131), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IGHM
• Synonyms: AGM1; MU; VH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC017356 edited
  GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCA
  GCTCCCAGATGGGTCCTGTCCCAGGTGCAGCTACAGCAGTGGGGCGCAGGACTGTTGAAG
  CCTTCGGAGACCCTGTCCCTCACCTGCGGTGTTTATGGTGGGTCCTTCAGTGGTTACTAT
  TGGAGCTGGATTCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGGAAATCAATCAT
  AGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACG
  TCCAAGAAGCAGCTCTCCCTGAAGTTGAGCTCTGTGAACGCCGCGGACACGGCTGTGTAT
  TACTGTGCGAGAGTTATTACTAGGGCGAGTCCTGGCACAGACGGGAGGTACGGTATGGAC
  GTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCCAACCCTT
  TTCCCCCTCGTCTCCTGTGAGAATTCCCCGTCGGATACGAGCAGCGTGGCCGTTGGCTGC
  CTCGCACAGGACTTCCTTCCCGACTCCATCACTTTCTCCTGGAAATACAAGAACAACTCT
  GACATCAGCAGCACCCGGGGCTTCCCATCAGTCCTGAGAGGGGGCAAGTACGCAGCCACC
  TCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGACGAACACGTGGTGTGC
  AAAGTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGATTGCCGAG
  CTGCCTCCCAAAGTGAGCGTCTTCGTCCCACCCCGCGACGGCTTCTTCGGCAACCCCCGC
  AAGTCCAAGCTCATCTGCCAGGCCACGGGTTTCAGTCCCCGGCAGATTCAGGTGTCCTGG
  CTGCGCGAGGGGAAGCAGGTGGGGTCTGGCGTCACCACGGACCAGGTGCAGGCTGAGGCC
  AAAGAGTCTGGGCCCACGACCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGAC
  TGGCTCAGCCAGAGCATGTTCACCTGCCGCGTGGATCACAGGGGCCTGACCTTCCAGCAG
  AATGCGTCCTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCC
  CCATCCTTTGCCAGCATCTTCCTCACCAAGTCCACCAAGTTGACCTGCCTGGTCACAGAC
  CTGACCACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAA
  ACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTTCAGCGCCGTGGGTGAGGCC
  AGCATCTGCGAGGATGACTGGAATTCCGGGGAGAGGTTCACGTGCACCGTGACCCACACA
  GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGG
  CCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACC
  ATCACGTGCCTGGTGACGGGCTTCTCTCCCGCGGACGTCTTCGTGCAGTGGATGCAGAGG
  GGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCC
  CCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAACACGGGG
  GAGACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGAGAGGACC
  GTGGACAAGTCCACCGAGGGGGAGGTGAGCGCCGACGAGGAGGGCTTTGAGAACCTGTGG
  GCCACCGCCTCCACCTTCATCGTCCTCTTCCTCCTGAGCCTCTTCTACAGTACCACCGTC
  ACCTTGTTCAAGGTGAAATGATCCCAACAGAAGAACATCGGAGACCAGAGAGAGGAACTC
  AAAGGGGCGCAGCCTCCGGGTCTGGGGTCCTGGCCTGCGTGGCCTGTTGGCACGTGTTTC
  TCTTCCCCGCCCGGCCTCCAGTTGTGTGCTCTCACACAGGCTTCCTTCTCGACCGGCAGG
  GGCTGGCTGGCTTGCAGGCCACGAGGTGGGCTCTACCCCACACTGCTTTGCTGTGTATAC
  GCTTGTTGCCCTGAAATAAATATGCACATTTTATCCATGAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC017356 unedited
  GTCCAAATTTTGTAATACGACTCAACTATAGGGCGGCCGCGAATTCGCACGAGGGTCATG
  GACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCC
  AGATGGGTCCTGTCCCAGGTGCAGCTACAGCAGTGGGGCGCAGGACTGTTGAAGCCTTCG
  GAGACCCTGTCCCTCACCTGCGGTGTTTATGGTGGGTCCTTCAGTGGTTACTATTGGAGC
  TGGATTCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGGAAATCAATCATAGTGGA
  AGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAG
  AAGCAGCTCTCCCTGAAGTTGAGCTCTGTGAACGCCGCGGACACGGCTGTGTATTACTGT
  GCGAGAGTTATTACTAGGGCGAGTCCTGGCACAGACGGGAGGTACGGTATGGACGTCTGG
  GGCCAAGGGACCACGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCCAACCCTTTTCCCC
  CTCGTCTCCTGTGAGAATTCCCCGTCGGATACGAGCAGCGTGGCCGTTGGCTGCCTCGCA
  CAGGACTTCCTTCCCGACTCCATCACTTTCTCCTGGAAATACAAGAACAACTCTGACATC
  AGCAGCACCCGGGGCTTCCCATCAGTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAG
  GTGCTGCTGCCTTCCCAGGACGTCATGCAGGNCACAGACGAACACGTGGTGTGCAAAGTC
  CAGCACCCNAACGCAACAAAGAAAAGACGTGCCCTTTCCAGTGATTGCCGAGCTGCCTCC
  CAAAGTGAGCGGTCTCGTCCCACCCCGCGACGGCTTNCTTCGGAACCCCCGCAGTCCAAG
  CTTATCTGGCAGGCCACGGTTTTAAGTCCCG
  
• Restriction Sites: Please inquire
• ACCN: BC017356
• Insert Size: 2179 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC017356.2
• RefSeq Size: 2170 bp
• Locus ID: 3507
• Cytogenetics: 14q32.33
• Gene Summary: Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]