DHX58 (NM_024119) Human Untagged Clone

CAT#: SC122954

DHX58 (untagged)-Human DEXH (Asp-Glu-X-His) box polypeptide 58 (DHX58)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHX58
• Synonyms: D11LGP2; D11lgp2e; LGP2; RLR-3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024119 edited
  AAGCAGGGCCGCCTTGGGCAGGCCTACGTGGTGGTGCAGGCGAGACCCAGGCTGGGCAAG
  GCGCAGTTTCAGTTTCCATCTTGGGTCTCTGAGCTGAGCAGAGTGGCACCAGGCTGAGTT
  AAGTGGGACTGCCCTGGGCAGACCTACCTACTAGAGCAGAATGGAGCTTCGGTCCTACCA
  ATGGGAGGTGATCATGCCTGCCCTGGAGGGCAAGAATATCATCATCTGGCTGCCCACGGG
  TGCCGGGAAGACCCGGGCGGCTGCTTATGTGGCCAAGCGGCACCTAGAGACTGTGGATGG
  AGCCAAGGTGGTTGTATTGGTCAACAGGGTGCACCTGGTGACCCAGCATGGTGAAGAGTT
  CAGGCGCATGCTGGATGGACGCTGGACCGTGACAACCCTGAGTGGGGACATGGGACCACG
  TGCTGGCTTTGGCCACCTGGCCCGGTGCCATGACCTGCTCATCTGCACAGCAGAGCTTCT
  GCAGATGGCACTGACCAGCCCCGAGGAGGAGGAGCACGTGGAGCTCACTGTCTTCTCCCT
  GATCGTGGTGGATGAGTGCCACCACACGCACAAGGACACCGTCTACAACGTCATCATGAG
  CCAGTACCTAGAACTTAAACTCCAGAGGGCACAGCCGCTACCCCAGGTGCTGGGTCTCAC
  AGCCTCCCCAGGCACTGGCGGGGCCTCCAAACTCGATGGGGCCATCAACCACGTCCTGCA
  GCTCTGTGCCAACTTGGACACGTGGTGCATCATGTCACCCCAGAACTGCTGCCCCCAGCT
  GCAGGAGCACAGCCAACAGCCTTGCAAACAGTACAACCTCTGCCACAGGCGCAGCCAGGA
  TCCGTTTGGGGACTTGCTGAAGAAGCTCATGGACCAAATCCATGACCACCTGGAGATGCC
  TGAGTTGAGCCGGAAATTTGGGACGCAAATGTATGAGCAGCAGGTGGTGAAGCTGAGTGA
  GGCTGCGGCTTTGGCTGGGCTTCAGGAGCAACGGGTGTATGCGCTTCACCTGAGGCGCTA
  CAATGACGCGCTGCTCATCCATGACACCGTCCGCGCCGTGGATGCCTTGGCTGCGCTGCA
  GGATTTCTATCACAGGGAGCACGTCACTAAAACCCAGATCCTGTGTGCCGAGCGCCGGCT
  GCTGGCCCTGTTCGATGACCGCAAGAATGAGCTGGCCCACTTGGCAACTCATGGCCCAGA
  GAATCCAAAACTGGAGATGCTGGAAAAGATCCTGCAAAGGCAGTTCAGTAGCTCTAACAG
  CCCTCGGGGTATCATCTTCACCCGCACCCGCCAAAGCGCACACTCCCTCCTGCTCTGGCT
  CCAGCAGCAGCAGGGCCTGCAGACTGTGGACATCCGGGCCCAGCTACTGATTGGGGCTGG
  GAACAGCAGCCAGAGCACCCACATGACCCAGAGGGACCAGCAAGAAGTGATCCAGAAGTT
  CCAAGATGGAACCCTGAACCTTCTGGTGGCCACGAGTGTGGCGGAGGAGGGGCTGGACAT
  CCCACATTGCAATGTGGTGGTGCGTTATGGGCTCTTGACCAATGAAATCTCCATGGTCCA
  GGCCAGGGGCCGTGCCCGGGCCGATCAGAGTGTATACGCGTTTGTAGCAACTGAAGGTAG
  CCGGGAGCTGAAGCGGGAGCTGATCAACGAGGCGCTGGAGACGCTGATGGAGCAGGCAGT
  GGCTGCTGTGCAGAAAATGGACCAGGCCGAGTACCAGGCCAAGATCCGGGATCTGCAGCA
  GGCAGCCTTGACCAAGCGGGCGGCCCAGGCAGCCCAGCGGGAGAACCAGCGGCAGCAGTT
  CCCAGTGGAGCACGTGCAGCTACTCTGCATCAACTGCATGGTGGCTGTGGGCCATGGCAG
  CGACCTGCGGAAGGTGGAGGGCACCCACCATGTCAATGTGAACCCCAACTTCTCGAACTA
  CTATAATGTCTCCAGGGATCCTGTGGTCATCAACAAAGTCTTCAAGGACTGGAAGCCTGG
  GGGTGTCATCAGCTGCAGGAACTGTGGGGAGGTCTGGGGTCTGCAGATGATCTACAAGTC
  AGTGAAGCTGCCAGTGCTCAAAGTCCGCAGCATGCTGCTGGAGACCCCTCAGGGGCGGAT
  CCAGGCCAAAAAGTGGTCCCGCGTGCCCTTCTCCGTGCCTGACTTTGACTTCCTGCAGCA
  TTGTGCCGAGAACTTGTCGGACCTCTCCCTGGACTGACCACCTCATTGCTGCAGTGCCCG
  GTTTGGGCTGTAGGGGGCGGGAGAGTCTGCAGCAGACTCCAGGCCCCTCCTTCCTGAATC
  ATCAGCTGTGGGCATCAGGCCCACCAGCCACACAGGAGTCCTGGGCACCCTGGCTTAGGC
  TCCCGCAATGGGAAAACAACCGGAGGGCCAGAGCTTAGTCCAGACCTACCTTGTACGCAC
  ATAGACATTTTCATATGCACTGGATGGAGTTAGGGAAACTGAGGCAAAAGAATTTGCCAT
  ACTGTACTCAGAATCACGACATTCCTTCCCTACCAAGGCCACTTCTATTTTTTGAGGCTC
  CTCATAAAAATAAATGAAAAAATGGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024119 unedited
  GCAGTTCGCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAAGCGGG
  CCGCCTTGGGCAGGCCTACGTGGTGGTGCAGGCGANGACCCAGCTGGGCAAGGCGCAGTT
  TCAGTTTCCATCTTGGGTCTCTGAGCTGAGCAGAGTGGCACCAGGCTGAGTTAAGTGGGA
  CTGCCCTGGGCAGACCTACCTACTAGAGCAGAATGGAGCTTCGGTCCTACCAATGGGAGG
  TGATCATGCCTGCCCTGGAGGGCAAGAATATCATCATCTGGCTGCCCACGGGTGCCGGGA
  AGACCCGGGCGGCTGCTTATGTGGCCAAGCGGCACCTAGAGACTGTGGATGGAGCCAAGG
  TGGTTGTATTGGTCAACAGGGTGCACCTGGTGACCCAGCATGGTGAAGAGTTCAGGCGCA
  TGCTGGATGGACGCTGGACCGTGACAACCCTGAGTGGGGACATGGGACCACGTGCTGGCT
  TTGGCCACCTGGCCCGGTGCCATGACCTGCTCATCTGCACAGCAGAGCTTCTGCAGATGG
  CACTGACCAGCCCCGAGGAGGAGGAGCACGTGGAGCTCACTGTCTTCTCCCTGATCGTGG
  TGGATGAGTGCCACCACACGCACAAGGACACCGTCTACAACGTCATCATGAGCCAGTACC
  TAGAACTTAAACTCCAGAGGGCACAGCCGCTACCCCAGGTGCTGGGGTCTCACAGCCTCC
  CCAGGCACTGGCGGGGCCTCCAAACTCGATGGGGGCCATCAACCACGTCCTGGCAGCTCT
  GTTGCCACCTTGGACACGTGGTGCATCATGTCACCCCCAAAACTGCTGCCCCCAGCTGCA
  GGAGCACAGCCAACAGCCTGGCAACAGTTCAACCCTCTGCCACAGGCCCAACCAGGTTTC
  TT
  
• Restriction Sites: Please inquire
• ACCN: NM_024119
• Insert Size: 2584 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024119.1, NP_077024.1
• RefSeq Size: 2613 bp
• RefSeq ORF: 2037 bp
• Locus ID: 79132
• UniProt ID: Q96C10
• Cytogenetics: 17q21.2
• Protein Pathways: RIG-I-like receptor signaling pathway
• Gene Summary: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.[UniProtKB/Swiss-Prot Function]