KCNMA1 (NM_002247) Human Untagged Clone

CAT#: SC122078

KCNMA1 (untagged)-Human potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNMA1
• Synonyms: bA205K10.1; BKTM; CADEDS; hSlo; IEG16; KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA; SLO1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002247, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAAATGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGAGGCAGCAGTCTTA
  GAATGAGTAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCCTCCTCCTCTTC
  CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC
  ATCCCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCCTGGCCTCCT
  CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT
  GTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATTAACAATGGCTCAAGCCAGGCGGATGGC
  ACTCTCAAACCAGTGGATGAAAAAGAGGAGGCAGTGGCCGCCGAGGTCGGCTGGATGACCTCCGTGAAGG
  ACTGGGCGGGGGTGATGATATCCGCCCAGACACTGACTGGCAGAGTCCTGGTTGTCTTAGTCTTTGCTCT
  CAGCATCGGTGCACTTGTAATATACTTCATAGATTCATCAAACCCAATAGAATCCTGCCAGAATTTCTAC
  AAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTTCTCTACTTCGGCTTGCGGTTTA
  TTGCAGCCAACGATAAATTGTGGTTCTGGCTGGAAGTGAACTCTGTAGTGGATTTCTTCACGGTGCCCCC
  CGTGTTTGTGTCTGTGTACTTAAACAGAAGTTGGCTTGGTTTGAGATTTTTAAGAGCTCTGAGACTGATA
  CAGTTTTCAGAAATTTTGCAGTTTCTGAATATTCTTAAAACAAGTAATTCCATCAAGCTGGTGAATCTGC
  TCTCCATATTTATCAGCACGTGGCTGACTGCAGCCGGGTTCATCCATTTGGTGGAGAATTCAGGGGACCC
  ATGGGAAAATTTCCAAAACAACCAGGCTCTCACCTACTGGGAATGTGTCTATTTACTCATGGTCACAATG
  TCCACCGTTGGTTATGGGGATGTTTATGCAAAAACCACACTTGGGCGCCTCTTCATGGTCTTCTTCATCC
  TCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATA
  CGGGGGCTCCTATAGTGCGGTTAGTGGAAGAAAGCACATTGTGGTCTGCGGACACATCACTCTGGAGAGT
  GTTTCCAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGACGTCAATGTGGAGATCGTTTTTCTTC
  ACAACATCTCCCCCAACCTGGAGCTTGAAGCTCTGTTCAAACGACATTTTACTCAGGTGGAATTTTATCA
  GGGTTCCGTCCTCAATCCACATGATCTTGCAAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTT
  GCCAACAAGTACTGCGCTGACCCGGATGCGGAGGATGCCTCGAATATCATGAGAGTAATCTCCATAAAGA
  ACTACCATCCGAAGATAAGAATCATCACTCAAATGCTGCAGTATCACAACAAGGCCCATCTGCTAAACAT
  CCCGAGCTGGAATTGGAAAGAAGGTGATGACGCAATCTGCCTCGCAGAGTTGAAGTTGGGCTTCATAGCC
  CAGAGCTGCCTGGCTCAAGGCCTCTCCACCATGCTTGCCAACCTCTTCTCCATGAGGTCATTCATAAAGA
  TTGAGGAAGACACATGGCAGAAATACTACTTGGAAGGAGTCTCAAATGAAATGTACACAGAATATCTCTC
  CAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTCAAGCTCCTAATG
  ATAGCCATTGAGTACAAGTCTGCCAACCGAGAGAGCCGTATATTAATTAATCCTGGAAACCATCTTAAGA
  TCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGCATTTTTTTACTG
  CAAGGCCTGTCATGATGACATCACAGATCCCAAAAGAATAAAAAAATGTGGCTGCAAACGGCTTGAAGAT
  GAGCAGCCGTCAACACTATCACCAAAAAAAAAGCAACGGAATGGAGGCATGCGGAACTCACCCAACACCT
  CGCCTAAGCTGATGAGGCATGACCCCTTGTTAATTCCTGGCAATGATCAGATTGACAACATGGACTCCAA
  TGTGAAGAAGTACGACTCTACTGGGATGTTTCACTGGTGTGCACCCAAGGAGATAGAGAAAGTCATCCTG
  ACTCGAAGTGAAGCTGCCATGACCGTCCTGAGTGGCCATGTCGTGGTCTGCATCTTTGGCGACGTCAGCT
  CAGCCCTGATCGGCCTCCGGAACCTGGTGATGCCGCTCCGTGCCAGCAACTTTCATTACCATGAGCTCAA
  GCACATTGTGTTTGTGGGCTCTATTGAGTACCTCAAGCGGGAATGGGAGACGCTTCATAACTTCCCCAAA
  GTGTCCATATTGCCTGGTACGCCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCAACCTCTGTGACA
  TGTGCGTTATCCTGTCAGCCAATCAGAATAATATTGATGATACTTCGCTGCAGGACAAGGAATGCATCTT
  GGCGTCACTCAACATCAAATCTATGCAGTTTGATGACAGCATCGGAGTCTTGCAGGCTAATTCCCAAGGG
  TTCACACCTCCAGGAATGGATAGATCCTCTCCAGATAACAGCCCAGTGCACGGGATGTTACGTCAACCAT
  CCATCACAACTGGGGTCAACATCCCCATCATCACTGAACTAGTGAACGATACTAATGTTCAGTTTTTGGA
  CCAAGACGATGATGATGACCCTGATACAGAACTGTACCTCACGCAGCCCTTTGCCTGTGGGACAGCATTT
  GCCGTCAGTGTCCTGGACTCACTCATGAGCGCGACGTACTTCAATGACAATATCCTCACCCTGATACGGA
  CCCTGGTGACCGGAGGAGCCACGCCGGAGCTGGAGGCTCTGATTGCTGAGGAAAACGCCCTTAGAGGTGG
  CTACAGCACCCCGCAGACACTGGCCAATAGGGACCGCTGCCGCGTGGCCCAGTTAGCTCTGCTCGATGGG
  CCATTTGCGGACTTAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAGCTCTGAAAACATATAATA
  TGCTTTGTTTTGGAATTTACCGGCTGAGAGATGCTCACCTCAGCACCCCCAGTCAGTGCACAAAGAGGTA
  TGTCATCACCAACCCGCCCTATGAGTTTGAGCTCGTGCCGACGGACCTGATCTTCTGCTTAATGCAGTTT
  GACCACAATGCCGGCCAGTCCCGGGCCAGCCTGTCCCATTCCTCCCACTCGTCGCAGTCCTCCAGCAAGA
  AGAGCTCCTCTGTTCACTCCATCCCATCCACAGCAAACCGACAGAACCGGCCCAAGTCCAGGGAGTCCCG
  GGACAAACAGAAGTACGTGCAGGAAGAGCGGCTTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002247 unedited
  GGCGGCCGCGAATTCGGCACCAGCCCTGCGCCGGCTGCCCGTTGCTAGCTATGGCAAATG
  GTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGAGGCAGCAGTCTTA
  GAATGAGTAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCCT
  CCTCCTCTTCCTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGC
  CCAAGATGGATGCGCTCATCATCCCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCC
  AACGCATGTGGTGGGCTTTCCTGGCCTCCTCCATGGTGACTTTCTTCGGGGGCCTCTTCA
  TCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGTGTGCTGCCACTGCGGGGGCA
  AGACGAAGGAGGCCCAGAAGATTAACAATGGCTCAAGCCAGGCGGATGGCACTCTCAAAC
  CAGTGGATGAAAAAGAGGAGGCAGTGGCCGCCGAGGTCGGCTGGATGACCTCCGTGAAGG
  ACTGGGCGGGGGTGATGATATCCGCCCAGACACTGACTGGCAGAGTCCTGGCTGTCTTAG
  TCTTTGCTCTCAGCATCGGTGCACTTGTAATATACTTCATAGATTCATCANACCCAATAG
  AATCCTGCCAGAATTTCTACAAAGATTTCACATTACAGATCGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002247 unedited
  CCCCCCTGACTTGGACCGCGGNCCGCATCCTACGACGCGGTTTTTTTTTTTTTTTTTTGG
  AACTCCTTTTTTTTTAATTTCACAAAAGTTTTCACACGGACAACGTTATAGAAGAAAACC
  CCCAGCAGTGGCTAGGTCATGCAGAACCATTAATTGTCATACCTTGGCCCATTCTATTCA
  TCCTTGTTGCACTTTAGAGAGAGAAGTAAGCTATGTGAGTTTTACAATGCTTTTAAACTG
  TCATATTTCCTGTTGAGCACTTTAACTGGCACATTCTTATAGTTATAAATGTTCTGAGGG
  CGTAACTTTATAACCTCCTTTGCAAAGAATGCATGAAGAGCTCTTCATGATCCAATACTA
  GGGGATACATATATATAGTTATATATATAGTTATATATATATAATTATATAGTTATATAT
  ATATAATTATATATAGTTTATATAAAGAGATAGTTATACATAATATAAATCAATATATAG
  AGGGACAAAAATAATTTGAAAAAATTCTTCCACTCATAGGGCTTGATAATTTACATGTAT
  ACAATTATTCCCCACCACAATACTGGTTTGAATACTACGCTGCCCCTGTCTTTGGTGTGG
  GGGAGGGTGGGAGGGCTCTCCCTGACCCCCCCCCATCGCTCATCAACACCGCTTTGCCTC
  CTTCTCCACAGGACGCCCCAATCCCCCACTCTCACGCTTTGCGGCCACCTTGTATCCCCA
  CCCCCACCCCCACCTCCTCACATCCCCAACCCTCTATTCCCACGGGGCACCCACGTGACC
  CGCTCCCCCCCCCCCTTTTCCCCCTACTCTATCCCCGCCCTCCCCCTCCCACTCACCCTC
  TCCCCTTCCCTCACCCCCTGCCTTTTTCATCCTATACTGCAATATTGTACATCCCAACCT
  CCACCACCATCTCCCTCCCACCCACCCCTCCACGTCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002247
• Insert Size: 6100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002247.2, NP_002238.2
• RefSeq Size: 6103 bp
• RefSeq ORF: 3537 bp
• Locus ID: 3778
• UniProt ID: Q12791
• Cytogenetics: 10q22.3
• Domains: BK_channel_a, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 3' UTR and has multiple coding region differences, compared to variant 1. The resulting isoform (b) contains a distinct C-terminus, compared to isoform a.