GRIK1 (NM_175611) Human Untagged Clone

CAT#: SC121962

GRIK1 (untagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRIK1
• Synonyms: EAA3; EEA3; GLR5; GluK1; gluR-5; GLUR5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121962 sequence for NM_175611 edited (data generated by NextGen Sequencing)
  ATGGAGCACGGCACACTCCTCGCCCAGCCCGGGCTCTGGACCAGGGACACCAGCTGGGCA
  CTCCTCTATTTCCTCTGCTATATCCTCCCTCAGACCGCCCCGCAAGTACTCAGGATCGGA
  GGGATTTTTGAAACAGTGGAAAATGAGCCTGTTAATGTTGAAGAATTAGCTTTCAAGTTT
  GCAGTCACCAGCATTAACAGAAACCGAACCCTGATGCCTAACACCACATTAACCTATGAC
  ATCCAGAGAATTAACCTTTTTGATAGTTTTGAAGCCTCGCGGAGAGCATGTGACCAGCTG
  GCTCTTGGTGTGGCTGCTCTCTTTGGCCCTTCCCATAGCTCCTCCGTCAGTGCTGTGCAG
  TCTATTTGCAATGCTCTCGAAGTTCCACACATACAGACCCGCTGGAAACACCCCTCGGTG
  GACAACAAAGATTTGTTTTACATCAACCTTTACCCAGATTATGCAGCTATCAGCAGGGCG
  ATCCTGGATCTGGTCCTCTATTACAACTGGAAAACAGTGACCGTGGTGTATGAAGACAGC
  ACAGGTCTAATTCGTCTACAAGAGCTCATCAAAGCTCCCTCCAGATATAATATTAAAATC
  AAAATCCGCCAGCTGCCCTCTGGGAATAAAGATGCCAAGCCTTTACTCAAGGAGATGAAG
  AAAGGCAAGGAGTTCTATGTGATATTTGATTGTTCACATGAAACAGCCGCTGAAATCCTT
  AAGCAGATTCTGTTCATGGGCATGATGACCGAGTACTATCACTACTTTTTCACAACCCTG
  GACTTATTTGCTTTGGATCTGGAACTCTATAGGTACAGTGGCGTAAACATGACCGGGTTT
  CGGCTGCTTAACATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAG
  AGACTGCAGGCCCCACCCAGGCCCGAGACTGGCCTTTTGGATGGCATGATGACAACTGAA
  GCGGCTCTGATGTACGATGCTGTGTACATGGTGGCCATTGCCTCGCACCGGGCATCCCAG
  CTGACCGTCAGCTCCCTGCAGTGCCATAGACATAAGCCATGGCGCCTCGGACCCAGATTT
  ATGAACCTGATCAAAGAGGCCCGGTGGGATGGCTTGACTGGGCATATCACCTTTAATAAA
  ACCAATGGCTTGAGGAAGGATTTTGATCTGGATATTATTAGTCTCAAAGAGGAAGGAACT
  GAAAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGAC
  AAGTCCAGCAATATCACTGATTCATTGGCCAACAGAACACTCATTGTCACCACCATTCTG
  GAAGAACCCTATGTTATGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTT
  GAAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTGGGTTTCATTTATGAT
  GTTAAACTAGTTCCCGATGGCAAATATGGGGCCCAGAATGACAAAGGGGAGTGGAACGGG
  ATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACC
  TACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGGCATCAGCATT
  CTCTACCGGAAGCCCAATGGTACCAATCCAGGCGTTTTCTCCTTCCTCAACCCCCTGTCT
  CCAGATATTTGGATGTATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTG
  ATTGCAAGGTTTACACCCTACGAGTGGTATAACCCCCACCCATGCAACCCTGACTCAGAC
  GTGGTGGAAAACAATTTTACTTTACTAAATAGTTTCTGGTTTGGAGTTGGAGCTCTCATG
  CAGCAAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGATATGG
  TGGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGGCTGCCTTCTTGACA
  GTAGAGAGAATGGAATCCCCCATAGATTCGGCAGATGATCTGGCAAAGCAAACCAAGATA
  GAATATGGGGCGGTTAGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCC
  ACCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAAC
  AGTGATGAGGGGATCCAGAGAGTGCTCACCACAGACTACGCGCTGCTGATGGAGTCCACC
  AGCATTGAGTATGTGACGCAGAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGAC
  TCCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGGATAAAATTACTATT
  GCTATTCTTCAACTCCAAGAAGAAGGGAAGCTGCATATGATGAAAGAGAAGTGGTGGCGT
  GGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATT
  GGAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTATTTGTAGCTATTGGA
  GAATTCATATACAAATCACGGAAGAATAATGATATTGAACAGTGTCTCTCTTTCAACGCT
  ATCATGGAAGAACTGGGAATCTCACTGAAGAATCAGAAAAAAATAAAGAAAAAGTCAAGA
  ACTAAGGGGAAATCTTCCTTCACAAGTATCCTTACTTGTCATCAGAGACGAACTCAGAGA
  AAAGAGACTGTGGCGTGA
  
  Clone variation with respect to NM_175611.2
  522 a=>c;1173 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175611 unedited
  GCTTGCTCAGAGCCCCTGCACCAACTCACCCTGTACCCTCTCTCCTTCTTCGTTAGTCTT
  CTTTCCCCCTTTTCCCTCCTCTGTCTGTGCCTATCCCCCGACTTTTGCATCTGACCAAAG
  GACGAATGAGGGAGACGTTCCTGCAGATCGGGGCAGCAACTTTCCTCAGCTGGTCTCTGG
  GCTCCGGGAGCCAGAGAGCGCTGATCCTCCGCGGTCTGCGGCCCATGGAAGAGGAGGAGG
  AGGAGCCGTGATGGGCTAGCGACAGCACTGAGGAGCCCCGAGAGAGCTCAGCCTTGCCAG
  CCAGCTCCGCGGTCCCACGCGGGTTCCCTCGAGCTCGCTCCGTGGGGAGCGCGCAGCGTG
  CTTGGAACCGGAGCATCCAGAGAGGATGAGGCGGGGACCCGGCCCAAGTTGGGTGCATCT
  CTCGGGCGTCCGGCAGCGGCTGTATCTCGGCATGAATTAAGAAGCTAGGAAGATGGAGCA
  CGGNACACTCCTCGCCCAGCCCGGGCTCTGGACCAGGGACACCAGCTGGGCACTCCTCTA
  TTTCCTCTGCTATATCCTCCCTCAGACCGCCCCGNCAGTACTCAGGATCGGAGGGATTTT
  TGAAACAGTGGAAAATGAGCCTGTTAATGGTGAAAGAATAGCTTTCAAGTTTGCAGTCAC
  CAGCATTAACAGAAACCCGACCCTGATGCCCTACACCACATTAACCTATGACATCCAGAG
  AATTACCTTTTTGATAGTTTTGAAACCTGCCGAGAGCATGTGACCAGCTGGGCTCTGTGG
  GGCTGCTCTCTTTGGCCCTTCCATAACTCCTCCGTCATGCTGGGCAGNCTATTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_175611 unedited
  TTAAAACCTGTTGTTTTCATTATTATTTAGNAAGGCACACACAACAATTTATTGGGGGAA
  AAAGAAAAAATGCAAAATAATTGAGCATACAAATTTATTTTAAAAGAACTGGTGTCACTT
  CCCAAGTCATATCTATGTGAGCTTTTTAAACTTTTGTATTTCTTATATCTGTATTCATAA
  TCAGAGTTATGGATATAGATATATGGAAACACATCACACACTCCTCAGAAATCCTTTCTC
  CAAAAATCTGTAGGGAATGCATCCTTTTTCTTCCTACAGGCGTTTCCTTGGATCACGCCA
  CAGTCTCTTTTCTCTGAGTTCGTCTCTGATGACAAGTAAGGATACTTGTGAAGGAAGATT
  TCCCCTTAGTTCTTGACTTTTTCTTTATTTTTTTCTGATTCTTCAGTGAGATTCCCAGTT
  CTTCCATGATAGCGTTGAAAGAGAGACACTGTTCAATATCATTATTCTTCCGTGATTTGT
  ATATGAATTCTCCAATAGCTACAAATACAGAAAGGACCAGTCCGGCAGCCAGAACAATGA
  AGATGCCTCCAATATTTTCCACTCCCAGGGCACTGGCTTCTTTGTTGTCTTCCTCGGGGC
  AGCCATTCCCACGCCACCACTTCTCTTTCATCATATGCAGCTTCCCTTCTTCTTGGAGTT
  GAAGAAATAGCATAGTAATTNTATCCCGGGTAGGAGAACCAATAGGTGTTCCCACTNCCG
  TACCTTTGNAGTAATGAGGCCCCCGATCTGAGTGAGGTTGCAGTTTCTCTGCGTCACATA
  CTCAATGCTGGTGGACTNCATCAGCAGCGCGTAGTCTGTGGTGAGCACTCTCTGGATCCC
  CTCATCACTGTTTCTTACCAGGGCGGTCTGCTGCCTGCTGCTCATGAAAGCCACATCTTC
  TNATAGGTGGAGAATTTTGATTTCTTGAAGAAGTCATTGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_175611
• Insert Size: 3870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175611.2, NP_783300.1
• RefSeq Size: 3489 bp
• RefSeq ORF: 2718 bp
• Locus ID: 2897
• UniProt ID: P39086
• Cytogenetics: 21q21.3
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2), also known as EAA3A, lacks an alternate in-frame exon in the 3' coding region compared to variant 3. It encodes isoform 2, which is shorter than isoform 3.