CACNA2D3 (NM_018398) Human Untagged Clone

CAT#: SC121942

CACNA2D3 (untagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 3 (CACNA2D3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CACNA2D3
• Synonyms: HSA272268
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018398 edited
  GGCAGGCGGGGCGGCGCGGAGCGGAGCAGGCAGCCCCGCGCGCTCGCCCACCGCCCGCTC
  CGCGCAGCTCCCCGCGGCCGCTCTCGTCGCCGCCGCAGCGGGCGCGTCGGAGGGAGCCCA
  GCATGGCCGGGCCGGGCTCGCCGCGCCGCGCGTCCCGGGGGGCCTCGGCGCTTCTCGCTG
  CCGCGCTTCTCTACGCCGCGCTGGGGGACGTGGTGCGCTCGGAGCAGCAGATACCGCTCT
  CCGTGGTGAAGCTCTGGGCCTCGGCTTTTGGTGGGGAGATAAAATCCATTGCTGCTAAGT
  ACTCCGGTTCCCAGCTTCTGCAAAAGAAATACAAAGAGTATGAGAAAGACGTTGCCATAG
  AAGAAATTGATGGCCTCCAACTGGTAAAGAAGCTGGCAAAGAACATGGAAGAGATGTTTC
  ACAAGAAGTCTGAGGCCGTCAGGCGTCTGGTGGAGGCTGCAGAAGAAGCACACCTGAAAC
  ATGAATTTGATGCAGACTTACAGTATGAATACTTCAATGCTGTGCTGATAAATGAAAGGG
  ACAAAGACGGGAATTTTTTGGAGCTGGGAAAGGAATTCATCTTAGCCCCAAATGACCATT
  TTAATAATTTGCCTGTGAACATCAGTCTAAGTGACGTCCAAGTACCAACGAACATGTACA
  ACAAAGACCCTGCAATTGTCAATGGGGTTTATTGGTCTGAATCTCTAAACAAAGTTTTTG
  TAGATAACTTTGACCGTGACCCATCTCTCATATGGCAGTACTTTGGAAGTGCAAAGGGCT
  TTTTTAGGCAGTATCCGGGGATTAAATGGGAACCAGATGAGAATGGAGTCATTGCCTTCG
  ACTGCAGGAACCGAAAATGGTACATCCAGGCAGCAACTTCTCCGAAAGACGTGGTCATTT
  TAGTTGACGTCAGTGGCAGCATGAAAGGACTCCGTCTGACTATCGCGAAGCAAACAGTCT
  CATCCATTTTGGATACACTTGGGGATGATGACTTCTTCAACATAATTGCTTATAATGAGG
  AGCTTCACTATGTGGAACCTTGCCTGAATGGAACTTTGGTGCAAGCCGACAGGACAAACA
  AAGAGCACTTCAGGGAGCATCTGGACAAACTTTTCGCCAAAGGAATTGGAATGTTGGATA
  TAGCTCTGAATGAGGCCTTCAACATTCTGAGTGATTTCAACCACACGGGACAAGGAAGTA
  TCTGCAGTCAGGCCATCATGCTCATAACTGATGGGGCGGTGGACACCTATGATACAATCT
  TTGCAAAATACAATTGGCCAGATCGAAAGGTTCGCATCTTCACATACCTCATTGGACGAG
  AGGCTGCGTTTGCAGACAATCTAAAGTGGATGGCCTGTGCCAACAAAGGATTTTTTACCC
  AGATCTCCACCTTGGCTGATGTGCAGGAGAATGTCATGGAATACCTTCACGTGCTTAGCC
  GGCCCAAAGTCATCGACCAGGAGCATGATGTGGTGTGGACCGAAGCTTACATTGACAGCA
  CTCTCCCTCAGGCACAAAAGCTGACTGATGATCAGGGCCCCGTCCTGATGACCACTGTAG
  CCATGCCTGTGTTTAGTAAGCAGAACGAAACCAGATCGAAGGGCATTCTTCTGGGAGTGG
  TTGGCACAGATGTCCCAGTGAAAGAACTTCTGAAGACCATCCCCAAATACAAGTTAGGGA
  TTCACGGTTATGCCTTTGCAATCACAAATAATGGGTATATCCTGACGCATCCGGAACTCA
  GGCTGCTGTACGAAGAAGGAAAAAAGCGAAGGAAACCTAACTATAGTAGCGTTGACCTCT
  CTGAGGTGGAGTGGGAAGACCGAGATGACGTGTTGAGAAATGCTATGGTGAATCGAAAGA
  CGGGGAAGTTTTCCATGGAGGTGAAGAAGACAGTGGACAAAGGGAAACGGGTTTTGGTGA
  TGACAAATGACTACTATTATACAGACATCAAGGGTACTCCTTTCAGTTTAGGTGTGGCGC
  TTTCCAGAGGTCATGGGAAATATTTCTTCCGAGGGAATGTAACCATCGAAGAAGGCCTGC
  ATGACTTAGAACATCCCGATGTGTCCTTGGCAGATGAATGGTCCTACTGCAACACTGACC
  TACACCCTGAGCACCGCCATCTGTCTCAGTTAGAAGCGATTAAGCTCTACCTAAAAGGCA
  AAGAACCTCTGCTCCAGTGTGATAAAGAATTGATCCAAGAAGTCCTTTTTGACGCGGTGG
  TGAGTGCCCCCATTGAAGCGTATTGGACCAGCCTGGCCCTCAACAAATCTGAAAATTCTG
  ACAAGGGCGTGGAGGTTGCCTTCCTCGGCACTCGCACGGGCCTCTCCAGAATCAACCTGT
  TTGTCGGGGCTGAGCAGCTCACCAATCAGGACTTCCTGAAAGCTGGCGACAAGGAGAACA
  TTTTTAACGCAGACCATTTCCCTCTCTGGTACCGAAGAGCCGCTGAGCAGATTCCAGGGA
  GCTTCGTCTACTCGATCCCATTCAGCACTGGACCAGTCAATAAAAGCAATGTGGTGACAG
  CAAGTACATCCATCCAGCTCCTGGATGAACGGAAATCTCCTGTGGTGGCAGCTGTAGGCA
  TTCAGATGAAACTTGAATTTTTCCAAAGGAAGTTCTGGACTGCCAGCAGACAGTGTGCTT
  CCCTGGATGGCAAATGCTCCATCAGCTGTGATGATGAGACTGTGAATTGTTACCTCATAG
  ACAATAATGGATTTATTTTGGTGTCTGAAGACTACACACAGACTGGAGACTTTTTTGGTG
  AGATCGAGGGAGCTGTGATGAACAAATTGCTAACAATGGGCTCCTTTAAAAGAATTACCC
  TTTATGACTACCAAGCCATGTGTAGAGCCAACAAGGAAAGCAGCGATGGCGCCCATGGCC
  TCCTGGATCCTTATAATGCCTTCCTCTCTGCAGTAAAATGGATCATGACAGAACTTGTCT
  TGTTCCTGGTGGAATTTAACCTCTGCAGTTGGTGGCACTCCGATATGACAGCTAAAGCCC
  AGAAATTGAAACAGACCCTGGAGCCTTGTGATACTGAATATCCAGCATTCGTCTCTGAGC
  GCACCATCAAGGAGACTACAGGGAATATTGCTTGTGAAGACTGCTCCAAGTCCTTTGTCA
  TCCAGCAAATCCCAAGCAGCAACCTGTTCATGGTGGTGGTGGACAGCAGCTGCCTCTGTG
  AATCTGTGGCCCCCATCACCATGGCACCCATTGAAATCAGGTATAATGAATCCCTTAAGT
  GTGAACGTCTAAAGGCCCAGAAGATCAGAAGGCGCCCAGAATCTTGTCATGGCTTCCATC
  CTGAGGAGAATGCAAGGGAGTGTGGGGGTGCGCCGAGTCTCCAAGCCCAGACAGTCCTCC
  TTCTGCTCCCTCTGCTTTTGATGCTCTTCTCAAGGTGACACTGACTGAGATGTTCTCTTA
  CTGACTGAGATGTTCTCTTGGCATGCTAAATCATGGATAAACTGTGAACCAAAATATGGT
  GCAACATACGAGACATGAATATAGTCCAACCATCAGCATCTCATCATGATTTTAAACTGT
  GCGTGATATAAACTCTTAAAGATATGTTGACAAAAAGTTATCTATCATCTTTTTACTTTG
  CCAGTCATGCAAATGTGAGTTTGCCACATGATAATCACCCTTCATCAGAAATGGGACCGC
  AAGTGGTAGGCAGTGTCCCTTCTGCTTGAAACCTATTGAAACCAATTTAAAACTGTGTAC
  TTTTTAAATAAAGTATATTAAAATCATAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018398 unedited
  ATTCGGCACGAGGGGCAGGCGGGGCGGCGCGGAGCAGGAGCAGCAGCCCCGCGCGCTCGC
  CCACCGCCCGCTCCGCGCAGCTCCCCGCGGCCGCTCTCGTCGCCGCCGCAGCGGGCGCGT
  CGGAGGGAGCCCAGCATGGCCGGGCCGGGCTCGCCGCGCCGCGCGTCCCGGGGGGCCTCG
  GCGCTTCTCGCTGCCGCGCTTCTCTACGCCGCGCTGGGGGACGTGGTGCGCTCGGAGCAG
  CAGATACCGCTCTCCGTGGTGAAGCTCTGGGCCTCGGCTTTTGGTGGGGAGATAAAATCC
  ATTGCTGCTAAGTACTCCGGTTCCCAGCTTCTGCAAAAGAAATACAAAGAGTATGAGAAA
  GACGTTGCCATAGAAGAAATTGATGGCCTCCAACTGGTAAAGAAGCTGGCAAAGAACATG
  GAAGAGATGTTTCACAAGAAGTCTGAGGCCGTCAGGCGTCTGGTGGAGGCTGCAGAAGAA
  GCACACCTGAAACATGAATTTGATGCAGACTTACAGTATGAATACTTCAATGCTGTGCTG
  ATAAATGAAAGGGACAAAGACGGGAATTTTTTGGAGCTGGGAAAGGAATTCATCTTAGCC
  CCAAATGACCATTNTAATAATTTGCCTGTGAACATCAGTCTAAGTGACGTCCAAGTACCA
  ACGAACATGTACAACAAAGACCCTGCAATTGTCAATGGGGTTTATTGGTCTGAATCTCTA
  AACAAGTTTTTGTAGATAACTTTGACCGTGACCCATCTCTCATATGGCAGTACTTTTGGA
  GTGCANAGGGCTTTTTTTAGCAGTATCCGGGGATTAATGGGGACCAGATGAGNATGGAGT
  CATTGCCTTCGACTGCAGGAACCGAAATGGTACATNCAGGCAGNAACTTTTCGAAAGACG
  GG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018398 unedited
  TCCCCCCCAATGACTATGTACCGCGGCCCGTATACTATGATCGATTTTCTTTTTTTTTTT
  TTTTTATGATTTTAATATACTTTATTTAAAAATACACAGTTTTAAATTGGTTTCAATAGG
  TTTCAAGCAGAAGGGACACTGCCTACCACTTGCGGTCCCATTTCTGATGAAGGGTGATTA
  TCATGTGGCAAACTCACATTTGCATGACTGGCAAAGTTAAAAGATGATAGATAACTTTTT
  GTCAACATATCTTTAAGAGTTTATATCACGCACAGTTTAAAATCATGATGAGATGCTGAT
  GGTTGGACTATATTCATGTCTCGTATGTTGCACCATATTTTGGTTCACAGTTTATCCATG
  ATTTAGCATGCCAAGAGAACATCTCAGTCAGTAAGAGAACATCTCAGTCAGTGTCACCTT
  GAGAAGAGCATCAAAAGCAGAGGGAGCAGAAGGAGGACTGTCTGGGCTTGGAGACTCGGC
  GCACCCCCACACTCCCTTGCATTCTCCTCAGGATGGAAGCCATGACAAGATTCTGGGCGC
  CTTCTGATCTTCTGGGCCTTTAGACGTTCACACTTAAGGGATTCATTATACCTGATTTCA
  ATGGGTGCCATGGTGATGGGGGCCACAGATTCCCAGAGGCCGCTGCTGTCCACCACCACC
  ATGAACAGGTTGCTGCCTTGGGATTGGCTGGATGACAAAGGACTTGGAACAATCTTTACA
  AACCATATTCCCGGGAGTTCCCTTTGAGGTGCCCTCACAAACCAATGGCTGGATATTTAA
  GAACACAAGGCCTCCAGGGTTCTGTTTCAACTTTCTGGGGCTTTACCTGGCCAATCGGGC
  GTGGCCCCCCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018398
• Insert Size: 3800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018398.2, NP_060868.2
• RefSeq Size: 3689 bp
• RefSeq ORF: 3276 bp
• Locus ID: 55799
• UniProt ID: Q8IZS8
• Cytogenetics: 3p21.1-p14.3
• Domains: VWA, Cache
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• Gene Summary: This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]