PPP2R1A (BC001537) Human Untagged Clone

CAT#: SC121908

PPP2R1A (untagged)-Homo sapiens, clone MGC:786 IMAGE:2987938, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R1A
• Synonyms: alpha isoform of regulatory subunit A, protein phosphatase 2; medium tumor antigen-associated 61 KDA protein; MGC786; PP2A, subunit A, PR65-alpha isoform; PP2A, subunit A, R1-alpha isoform; PR65A; protein phosphatase 2 (formerly 2A), regulatory subunit A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC001537, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGCCGACGGCGACGACTCGCTGTACCCCATCGCGGTGCTCATAGACGAACTCCGCAATGAGG
  ACGTTCAGCTTCGCCTCAACAGCATCAAGAAGCTGTCCACCATCGCCTTGGCCCTTGGGGTTGAAAGGAC
  CCGAAGTGAGCTTCTGCCTTTCCTTACAGATACCATCTATGATGAAGATGAGGTCCTCCTGGCCCTGGCA
  GAACAGCTGGGAACCTTCACTACCCTGGTGGGAGGCCCAGAGTACGTGCACTGCCTGCTGCCACCGCTGG
  AGTCGCTGGCCACAGTGGAGGAGACAGTGGTGCGGGACAAGGCAGTGGAGTCCTTACGGGCCATCTCACA
  CGAGCACTCGCCCTCTGACCTGGAGGCGCACTTTGTGCCGCTAGTGAAGCGGCTGGCGGGCGGCGACTGG
  TTCACCTCCCGCACCTCGGCCTGCGGCCTCTTCTCCGTCTGCTACCCCCGAGTGTCCAGTGCTGTGAAGG
  CGGAACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACCCCCATGGTGCGGCGGGCCGCAGCCTC
  CAAGCTGGGGGAGTTTGCCAAGGTGCTGGAGCTGGACAACGTCAAGAGTGAGATCATCCCCATGTTCTCC
  AACCTGGCCTCTGACGAGCAGGACTCGGTGCGGCTGCTGGCGGTGGAGGCGTGCGTGAACATCGCCCAGC
  TTCTGCCCCAGGAGGATCTGGAGGCCCTGGTGATGCCCACTCTGCGCCAGGCCGCTGAAGACAAGTCCTG
  GCGCGTCCGCTACATGGTGGCTGACAAGTTCACAGAGCTCCAGAAAGCAGTGGGGCCTGAGATCACCAAG
  ACAGACCTGGTCCCTGCCTTCCAGAACCTGATGAAAGACTGTGAGGCCGAGGTGAGGGCCGCAGCCTCCC
  ACAAGGTCAAAGAGTTCTGTGAAAACCTCTCAGCTGACTGTCGGGAGAATGTGATCATGTCCCAGATCTT
  GCCCTGCATCAAGGAGCTGGTGTCCGATGCCAACCAACATGTCAAGTCTGCCCTGGCCTCAGTCATCATG
  GGTCTCTCTCCCATCTTGGGCAAAGACAACACCATCGAGCACCTCTTGCCCCTCTTCCTGGCTCAGCTGA
  AGGATGAGTGCCCTGAGGTACGGCTGAACATCATCTCTAACCTGGACTGTGTGAACGAGGTGATTGGCAT
  CCGGCAGCTGTCCCAGTCCCTGCTCCCTGCCATTGTGGAGCTGGCTGAGGACGCCAAGTGGCGGGTGCGG
  CTGGCCATCATTGAGTACATGCCCCTCCTGGCTGGACAGCTGGGAGTGGAGTTCTTTGATGAGAAACTTA
  ACTCCTTGTGCATGGCCTGGCTTGTGGATCATGTATATGCCATCCGCGAGGCAGCCACCAGCAACCTGAA
  GAAGCTAGTGGAAAAGTTTGGGAAGGAGTGGGCCCATGCCACAATCATCCCCAAGGTCTTGGCCATGTCC
  GGAGACCCCAACTACCTGCACCGCATGACTACGCTCTTCTGCATCAATGTGCTGTCTGAGGTCTGTGGGC
  AGGACATCACCACCAAGCACATGCTACCCACGGTTCTGCGCATGGCTGGGGACCCGGTTGCCAATGTCCG
  CTTCAATGTGGCCAAGTCTCTGCAGAAGATAGGGCCCATCCTGGACAACAGCACCTTGCAGAGTGAAGTC
  AAGCCCATCCTAGAGAAGCTGACCCAGGACCAGGATGTGGACGTCAAATACTTTGCCCAGGAGGCTCTGA
  CTGTTCTGTCTCTCGCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC001537 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCTGACAGGAAAGGACGGA
  GCCAAGATGGCGGCGGCCGACGGCGACGACTCGCTGTACCCCATCGCGGTGCTCATAGAC
  GAACTCCGCAATGAGGACGTTCAGCTTCGCCTCAACAGCATCAAGAAGCTGTCCACCATC
  GCCTTGGCCCTTGGGGTTGAAAGGACCCGAAGTGAGCTTCTGCCTTTCCTTACAGATACC
  ATCTATGATGAAGATGAGGTCCTCCTGGCCCTGGCAGAACAGCTGGGAACCTTCACTACC
  CTGGTGGGAGGCCCAGAGTACGTGCACTGCCTGCTGCCACCGCTGGAGTCGCTGGCCACA
  GTGGAGGAGACAGTGGTGCGGGACAAGGCAGTGGAGTCCTTACGGGCCATCTCACACGAG
  CACTCGCCCTCTGACCTGGAGGCGCACTTTGTGCCGCTAGTGAAGCGGCTGGCGGGCGGC
  GACTGGTTCACCTCCCGCACCTCGGCCTGCGGCCTCTTCTCCGTCTGCTACCCCCGAGTG
  TCCAGTGCTGTGAAGGCGGAACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACC
  CCCATGGTGCGGCGGGCCGCAGCCTNCAAGCTGGNGGAGTTTGCCAAGGTGCTGGAGCTG
  GNACACGTCAAGAGTGAGATCATCCCCCATGTCTCCAACCTGGCCTCTGACGAGCANGAC
  NNTCGTGCGCTGCTGGCNGNGGNNAGCTTGCCTAAACATCNNCCAGCTTCTGCCCCAGGA
  GATCTGGAGGCCCTGNGGATGCCCACTCTGCGCCACGCCGCTGAAAACAGTCCTGGCGCG
  TCCGCTACATGTTGCTGACAAGTCACAGAGCTCAAAAGCAGGGGGGCTGAAATCACCAGA
  AGAACTGGTCCCTGCTCCAAACCTGATGAAGACTGGAGGCGAGGTGANGCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC001537 unedited
  TTTCTCTGTGAAGGGGGAAATAAAGGATAAATAAAACGGCACAGTTGACACACAAAAAAA
  AACCAATGATGGGGAGGACGGGAGGTGGAGAAGTAAATGGGGGAGGGGTTCCCATTACAG
  CAGCAGGATCCAGTGACCCGGGATGCTCACATCTCTCCCTGACGTGGGCGGAGTAGCCCC
  TTCCTCCCAAGGTCACTGTCCTGTCCAACCCCGTGCTCCCCTAGCCCGTTGGGAGGTGGA
  CAGTGAGACATCTTCCCAGGCTGGGGAGAGGAGGAACCGTGCTGGGGGAAGGGGCCTGGG
  GTCAGACCATGCACAGGGAGTGACAGCCAAAGGCCCCCCAGTGTCTCCCCAAAGAGGGAC
  ACGTGGGGGTTGGAGGGTGGACACCAGAGGCCAGCGTTTGCTCCTCTTCCAGCATCAGGC
  GAGAGACAGAACAGTCAGAGCCTCCTGGGCAAAGTATTTGACGTCCACATCCTGGTCCTG
  GGTCAGCTTCTCTAGGATGGGCTCGACTTCACTCTGCCAGGTGCTGTTGCCCAGGATGGG
  CCCTATCCTCTGAACAGACTTGGCCACATTGAAGCCGGACATTGGCACCCGGTCCCCCAC
  CTTGCCCATTACCGTGGGCACCCTGTGCTTGTTGGTGAGGTCTTGCTACAGACCTATTAC
  GTACAATTGCGCCTAAAAACTTTTCCCTGCCGCTCCGGTTAACTGGGCCCTCCCGCCTTG
  GCCCACACCTTTGGGAAAATTTGCCCTGGGCCCCCTCCTTTCCCAACTTTTCCACTACCC
  TTTTCAGGTTTCTCGGTGCTGTCTCCGCGTGGGTTTTACTCATTCCCAACCTCGCCACTC
  ACTCGATTACCCCCCCACACCAACTTCCCCCCCCCCTTCTCCCGGAGTTATTTACCCACA
  CGGTCACCCCCCTCTCCTGGCGTCCCCCCCACCCTTCATCCCTGAACCCCCCTGGACACT
  TCTCCGCCCTCCTTTCTCCCATTCTCTCATCCTCCTCCTCCCCAGG
  
• Restriction Sites: NotI-NotI
• ACCN: BC001537
• Insert Size: 2390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC001537.2, AAH01537.1
• RefSeq Size: 2282 bp
• Locus ID: 5518
• Cytogenetics: 19q13.41
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
• Gene Summary: This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]