PNPLA4 (NM_004650) Human Untagged Clone

CAT#: SC121700

PNPLA4 (untagged)-Human patatin-like phospholipase domain containing 4 (PNPLA4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PNPLA4
• Synonyms: DXS1283E; GS2; iPLA2eta
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121700 sequence for NM_004650 edited (data generated by NextGen Sequencing)
  ATGAAGCACATCAACCTATCATTTGCAGCGTGTGGATTTCTGGGCATTTACCACTTGGGG
  GCAGCATCTGCACTTTGCAGACATGGCAAAAAACTTGTGAAGGATGTCAAAGCCTTCGCT
  GGGGCGTCTGCGGGATCGTTGGTTGCTTCTGTTCTGCTAACAGCACCAGAAAAAATAGAG
  GAATGTAACCAATTTACCTACAAGTTTGCCGAAGAAATCAGAAGGCAGTCTTTCGGGGCA
  GTAACGCCCGGTTATGACTTCATGGCCCGACTAAGAAGTGGGATGGAGTCGATTCTTCCT
  CCCAGCGCTCACGAGCTGGCCCAGAACCGACTGCACGTATCCATCACCAACGCCAAAACC
  AGAGAAAATCACTTAGTCTCCACTTTTTCCTCCAGGGAGGACCTCATTAAGGTCCTCCTA
  GCCAGCAGTTTTGTGCCCATTTATGCAGGACTGAAGCTAGTGGAATACAAAGGGCAGAAG
  TGGGTGGACGGAGGCCTCACCAACGCTCTTCCCATCCTGCCCGTCGGCCGGACAGTAACC
  ATCTCCCCCTTCAGTGGACGACTGGACATCTCCCCGCAGGACAAAGGGCAGCTAGATCTG
  TATGTTAATATCGCCAAGCAGGATATCATGTTGTCCCTGGCAAACCTGGTGAGACTCAAC
  CAAGCCCTTTTTCCCCCAAGCAAGAGGAAAATGGAATCTTTGTATCAGTGTGGTTTTGAT
  GACACTGTTAAGTTTTTACTTAAAGAAAATTGGTTTGAATAA
  
  Clone variation with respect to NM_004650.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004650 unedited
  TTGTAATACGACTCACTTAGGGCGGCCGCGACTTCGGCACGAGGCAGGACTTTCCTGGGC
  CGGCTCGCGGAGAGCGTAGCGCGGCCTTGGTGGCGGAATGGCGTTGAGTGACGGCCCGGC
  CCCGCCATCTGGTTAAAGGGACTCGTTCACACGGAAGTGTCCCGGGGCTGCATTGTGCTA
  CAGCTAGAATGAAGCACATCAACCTATCATTTGCAGCGTGTGGATTTCTGGGCATTTACC
  ACTTGGGGGCAGCATCTGCACTTTGCAGACATGGCAAAAAACTTGTGAAGGATGTCAAAG
  CCTTCGCTGGGGCGTCTGCGGGATCGTTGGTTGCTTCTGTTCTGCTAACAGCACCAGAAA
  AAATAGAGGAATGTAACCAATTTACCTACAAGTTTGCCGAAGAAATCAGAAGGCAGTCTT
  TCGGGNGCAGTAACGCCCGGTTATGACTTCATGGCCCACTAAGAAGTGGGAAGGGAGTCG
  ATTCTTCCTCCCAGCGCTCACGAGCTGGCCCAGAACCGACTGCACGTATCCATCACCAAC
  GCCAAAACCAGAGAAAATCACTTAGTCTCCACTTTTCCTTNGGGGGGAAGACCTCATTTT
  AGGTCCTCTACCCANCAAGGTTTTTGTGCCCATTNATGCANGACTGAAGCTAGTGGATAC
  AAGGCCAAAGTGGGGGGGACACGGAGCCTCACCACGCTCTTCCATNCCTGCCGTCGGCCG
  GACAGTACCATCTCCCCTTCAGTGGACGACTGGACATCTCCCCGCAGGACAAGGCAGCTA
  ATTTNTGTATGTTATATCGCCAGCAGATATAAAGGGTTGTCCTGGCAAACCTGGTGAGAT
  CAACCCAGCCTTTTTCCCCAGCAGAAGAAATGGATCTTGTTANNNNNGGGGTTTTTAAAA
  CTTTGTAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004650
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004650.1, NP_004641.1
• RefSeq Size: 2809 bp
• RefSeq ORF: 762 bp
• Locus ID: 8228
• UniProt ID: P41247
• Cytogenetics: Xp22.31
• Protein Pathways: Retinol metabolism
• Gene Summary: This gene encodes a member of the patatin-like family of phospholipases. The encoded enzyme has both triacylglycerol lipase and transacylase activities and may be involved in adipocyte triglyceride homeostasis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome Y. [provided by RefSeq, Feb 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.