Neurexin 1 (NRXN1) (NM_138735) Human Untagged Clone

CAT#: SC120579

NRXN1 (untagged)-Human neurexin 1 (NRXN1), transcript variant beta

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NRXN1
• Synonyms: Hs.22998; PTHSL2; SCZD17
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_138735 edited
  GCTGTTTGGAGACTGCAGATTTGCATAGAGCCCCTTGCTTCACCAGCGTGTGTGGTTTTT
  CTTTTCTTTTTTTCTTTTTCTTTTTCTTTTTCTGTTTCTCTCCCTTACTCCTTCGTCTGC
  ATCTTCTTCTCAGCTGCCTTTCCGCCGTTCGATTCCCACTTCCTTCAGAAGGGCGCACTC
  TTCCCTCTGAACCCCTCGCCGGGCGTAAGTGTCAGGAGGCGGCGGACGCGGAGATTGCCT
  CGGGAGCAGGCGATGCGCGCCGCTGCTCCGCGCGCTGCCCGGGGGAGGCTGGCGCTAGCG
  GGCGAGCCGGCCGGGGCTGAATGGAGGGAAGGGGCGCCTGGATTCCATGGGGTCTGCTTC
  TGTCTTGAAAGGAGGTTGGGCCGGCGAAGTGGCCTCCCTAGTCCCCGCACACAAAGCTAA
  ATGGATTTACCTAGTGGATTCGCGGTGGATGTCTGTCATGTAGAAGTGAGGACCTTCCAG
  GAGGAGCTTTAAACAATTTCTCGGCCCCCCACCCGCCGGCACGCACTCTCGCCCGAAACT
  CTTTGGGAGTATCTCTCGAGAACCTGGAGCCCTGGAAATCTGGGAGCAGCCACCCGCTCC
  GCGCTTGCTGTTCCCGGGGCTGCCTTTCCGGACAAGCCCCTCTCCAGCTGCATTCGCCTC
  GGCTATGTTTCGGATTGTTTGGGGTGCTAGGCACGGCGGACTCCAGGACCCCGAGAAGAC
  TGTCCGAAGGAGGGAGAGGATGGGTGCCCTGGCGCGGTGAGGCGGCCGGCCCCTCAGCCC
  ACTCCGCGCCGCCCGCTCCCCTGCGCCCTCTCCTCTCCCCGCGCCCCAAACTTTGCCTCC
  CGCGGCGGCTGCCCCTCGGCGGGCGCCCCGCCATGTACCAGAGGATGCTCCGGTGCGGCG
  CCGAGCTGGGCTCGCCCGGGGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGCAGGGGGGC
  GCCTGGCCCTGCTTTGGATAGTCCCGCTCACCCTCAGCGGCCTCCTAGGAGTGGCGTGGG
  GGGCATCCAGTTTGGGAGCGCACCACATCCACCATTTCCATGGCAGCAGCAAGCATCATT
  CAGTGCCTATTGCAATCTACAGGTCACCGGCATCCTTGCGAGGCGGACACGCTGGGACGA
  CATATATCTTTAGCAAAGGTGGTGGACAAATCACGTATAAGTGGCCTCCTAATGACCGAC
  CCAGTACACGAGCAGACAGACTGGCCATAGGTTTTAGCACTGTTCAGAAAGAAGCCGTAT
  TGGTGCGAGTGGACAGTTCTTCAGGCTTGGGTGACTACCTAGAACTGCATATACACCAGG
  GAAAAATTGGAGTTAAGTTTAATGTTGGGACAGATGACATCGCCATTGAAGAATCCAATG
  CAATCATTAATGATGGGAAATACCATGTAGTTCGTTTCACGAGGAGTGGTGGCAATGCCA
  CGTTGCAGGTGGACAGCTGGCCAGTGATCGAGCGCTACCCTGCAGGGCGTCAGCTCACAA
  TCTTCAATAGCCAAGCAACCATAATAATTGGCGGGAAAGAGCAGGGCCAGCCCTTCCAGG
  GCCAGCTCTCTGGGCTGTACTACAATGGCTTGAAAGTTCTGAATATGGCAGCCGAAAACG
  ATGCCAACATCGCCATAGTGGGAAATGTGAGACTGGTTGGTGAAGTGCCTTCCTCTATGA
  CAACTGAGTCAACAGCCACTGCCATGCAATCAGAGATGTCCACATCAATTATGGAGACTA
  CCACGACCCTGGCTACTAGCACAGCCAGAAGAGGAAAGCCCCCGACAAAAGAACCCATTA
  GCCAGACCACAGATGACATCCTTGTGGCCTCAGCAGAGTGTCCCAGCGATGATGAGGACA
  TTGACCCCTGTGAGCCGAGCTCAGGTGGGTTAGCCAACCCAACCCGAGCAGGCGGCAGAG
  AGCCGTATCCAGGCTCAGCAGAAGTGATCCGGGAGTCCAGCAGCACCACGGGTATGGTCG
  TTGGGATAGTAGCCGCTGCCGCCCTGTGCATCCTTATCCTCCTCTATGCCATGTACAAGT
  ACAGAAACCGGGATGAAGGCTCATACCATGTGGACGAGAGTCGAAACTACATCAGTAACT
  CAGCACAGTCCAATGGGGCTGTTGTAAAGGAGAAACAACCCAGCAGTGCGAAAAGCTCCA
  ACAAAAATAAGAAAAACAAGGATAAAGAGTATTATGTCTGATCCCAAGATCTTAAATGGA
  CACTTGTATAGAAATAGTCTTCATTTTATCTGAGACATAATATAAACTTATTTACTTTCC
  TTTTTATGAAGCACATACAAAAGAAGACAGGGAATGCAATCAGGAAGGAAAGACTTTTTA
  AAAAATAAAAACAAGTATCTCATGCTCTTGTTTCTCAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138735
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The insert of this clone has been fully sequenced and found to be perfect match to NM_138735.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138735.1, NP_620072.1
• RefSeq Size: 3712 bp
• RefSeq ORF: 1329 bp
• Locus ID: 9378
• UniProt ID: P58400
• Cytogenetics: 2p16.3
• Domains: LamG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are cell-surface receptors that bind neuroligins to form Ca(2+)-dependent neurexin/neuroligin complexes at synapses in the central nervous system. This complex is required for efficient neurotransmission and is involved in the formation of synaptic contacts. Three members of this gene family have been studied in detail and are estimated to generate over 3,000 variants through the use of two alternative promoters (alpha and beta) and extensive alternative splicing in each family member. Recently, a third promoter (gamma) was identified for this gene in the 3' region. Mutations in this gene are associated with Pitt-Hopkins-like syndrome-2 and may contribute to susceptibility to schizophrenia. [provided by RefSeq, Aug 2016]
  Transcript Variant: This variant (beta1) results from the use of an alternate promoter, referred to as beta. It contains an alternate 5' terminal exon and lacks most of the 5' exons and another exon in the 3' region compared to variant alpha2. The resulting isoform (beta1) is shorter with a distinct N-terminus lacking the EGF-like domain and containing fewer laminin G domains compared to isoform alpha2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.