RPL34 (NM_033625) Human Untagged Clone

CAT#: SC120119

RPL34 (untagged)-Human ribosomal protein L34 (RPL34), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL34
• Synonyms: L34
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120119 sequence for NM_033625 edited (data generated by NextGen Sequencing)
  ATGGTCCAGCGTTTGACATACCGACGTAGGCTTTCCTACAATACAGCCTCTAACAAAACT
  AGGCTGTCCCGAACCCCTGGTAATAGAATTGTTTACCTTTATACCAAGAAGGTTGGGAAA
  GCACCAAAATCTGCATGTGGTGTGTGCCCAGGCAGACTTCGAGGGGTTCGTGCTGTAAGA
  CCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCAGCAGGGCCTATGGTGGT
  TCCATGTGTGCTAAATGTGTTCGTGACAGGATCAAGCGTGCTTTCCTTATCGAGGAGCAG
  AAAATCGTTGTGAAAGTGTTGAAGGCACAAGCACAGAGTCAGAAAGCTAAATAA
  
  Clone variation with respect to NM_033625.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033625 unedited
  TGTTCACCATATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGGGCACT
  CAGAATGGTCCAGCGTTTGACATACCGACGTAGGCTTTCCTACAATACAGCCTCTAACAA
  AACTAGGCTGTCCCGAACCCCTGGTAATAGAATTGTTTACCTTTATACCAAGAAGGTTGG
  GAAAGCACCAAAATCTGCATGTGGTGTGTGCCCAGGCAGACTTCGAGGGGTTCGTGCTGT
  AAGACCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCAGCAGGGCCTATGG
  TGGTTCCATGTGTGCTAAATGTGTTCGTGACAGGATCAAGCGTGCTTTCCTTATCGAGGA
  GCAGAAAATCGTTGTGAAAGTGTTGAAGGCACAAGCACAGAGTCAGAAAGCTAAATAAAA
  AAATGAAACTTTTTTGAGTAATAAAAATGAAAAGACGCTGCAAAAAAAAAAAAAAAAAAC
  TCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCC
  CTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAG
  CCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATAAT
  TATGGGGTGGAGGGGGTGGGTATGGGAACAAGGGGCAAAGTTGGGAAGACAACCTGTAAG
  GCCTGCGGGGGCTATGGGGACCAACCTGAAGTGCAGTGGCACCATCTTGGCTCACTGCAA
  CTCCGCCTCCTGGTTTCAAGCGATCCTTCTGCCCTCACCTCCGGATTGGTGGGGATCCAG
  GCATGCATGACCAGGCTCACTTATTTTTGGTTTTTTGGAAAACCGGGGTTTCCCCTTTTG
  GCCAGCCTGTTTTCCACTCCCAATTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033625 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAGCGTCTTTTCAT
  TTTTATTACTCAAAAAAGTTTCATTTTTTTATTTAGCTTTCTGACTCTGTGCTTGTGCCT
  TCAACACTTTCACAACGATTTTCTGCTCCTCGATAAGGAAAGCACGCTTGATCCTGTCAC
  GAACACATTTAGCACACATGGAACCACCATAGGCCCTGCTGACATGTTTCTTTGTTTTGG
  ACAATCTCATAAGAACTTTAGGTCTTACAGCACGAACCCCTCGAAGTCTGCCTGGGCACA
  CACCACATGCAGATTTTGGTGCTTTCCCAACCTTCTTGGTATAAAGGTAAACAATTCTAT
  TACCAGGGGTTCGGGACAGCCTAGTTTTGTTAGAGGCTGTATTGTAGGAAAGCCTACGTC
  GGTATGTCAAACGCTGGACCATTCTGAGTGCCCCTCGTGCCGAATTCGCGGCCGCCCTAT
  AGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTC
  CCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGG
  AAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACT
  TGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGCCAAAACCGCAT
  CACCATGGTAATAGCGATGACTAATACGTANATGTACTGCCAAGTAGGAAAGTCCCCGTA
  GGTCATGTACTTGGCATAATGCCACGCCGGCCATTTACCGTCATTGACGTCAATAGGGGC
  CGACTGGNCATATGAACACCTGAGTACTGCCAGTGGGCAGTTACCGTAATACTCCCCCAT
  TGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033625
• Insert Size: 430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033625.1, NP_296374.1
• RefSeq Size: 869 bp
• RefSeq ORF: 354 bp
• Locus ID: 6164
• UniProt ID: P49207
• Cytogenetics: 4q25
• Domains: Ribosomal_L34e
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Overexpression of this gene has been observed in some cancer cells. Alternative splicing results in multiple transcript variants, all encoding the same isoform. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (2) represents the longest transcript. Variants 1-6 all encode the same protein.