Fumarylacetoacetate hydrolase (FAH) (NM_000137) Human Untagged Clone

CAT#: SC120070

FAH (untagged)-Human fumarylacetoacetate hydrolase (fumarylacetoacetase) (FAH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Fumarylacetoacetate hydrolase
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120070 sequence for NM_000137 edited (data generated by NextGen Sequencing)
  ATGTCCTTCATCCCGGTGGCCGAGGATTCCGACTTCCCCATCCACAACCTGCCCTACGGC
  GTCTTCTCGACCAGAGGCGACCCAAGACCGAGGATAGGTGTGGCCATTGGCGACCAGATC
  CTGGACCTCAGCATCATCAAGCACCTCTTTACTGGTCCTGTCCTCTCCAAACACCAGGAT
  GTCTTCAATCAGCCTACACTCAACAGCTTCATGGGCCTGGGTCAGGCTGCCTGGAAGGAG
  GCGAGAGTGTTCTTGCAGAACTTGCTCTCTGTGAGCCAAGCCAGGCTCAGAGATGACACC
  GAACTTCGGAAGTGTGCATTCATCTCCCAGGCTTCTGCCACGATGCACCTTCCAGCCACC
  ATAGGAGACTACACAGACTTCTATTCCTCTCGGCAGCATGCTACCAACGTCGGAATCATG
  TTCAGGGACAAGGAGAATGCGTTGATGCCAAATTGGCTGCACTTACCAGTGGGCTACCAT
  GGCCGTGCCTCCTCTGTCGTGGTGTCTGGCACCCCAATCCGAAGGCCCATGGGACAGATG
  AAACCTGATGACTCTAAGCCTCCCGTATATGGTGCCTGCAAGCTCTTGGACATGGAGCTG
  GAAATGGCTTTTTTTGTAGGCCCTGGAAACAGATTGGGAGAGCCGATCCCCATTTCCAAG
  GCCCATGAGCACATTTTTGGAATGGTCCTTATGAACGACTGGAGTGCACGAGACATTCAG
  AAGTGGGAGTATGTCCCTCTCGGGCCATTCCTTGGGAAGAGTTTTGGGACCACTGTCTCT
  CCGTGGGTGGTGCCCATGGATGCTCTCATGCCCTTTGCTGTGCCCAACCCGAAGCAGGAC
  CCCAGGCCCCTGCCGTATCTGTGCCATGACGAGCCCTACACATTTGACATCAACCTCTCT
  GTTAACCTGAAAGGAGAAGGAATGAGCCAGGCGGCTACCATATGCAAGTCCAATTTTAAG
  TACATGTACTGGACGATGCTGCAGCAGCTCACTCACCACTCTGTCAACGGCTGCAACCTG
  CGGCCGGGGGACCTCCTGGCTTCTGGGACCATCAGCGGGCCGGAGCCAGAAAACTTCGGC
  TCCATGTTGGAACTGTCGTGGAAGGGAACGAAGCCCATAGACCTGGGGAATGGTCAGACC
  AGGAAGTTTCTGCTGGACGGGGATGAAGTCATCATAACAGGGTACTGCCAGGGGGATGGT
  TACCGCATCGGCTTTGGCCAGTGTGCTGGAAAAGTGCTGCCTGCTCTCCTGCCATCATGA
  
  Clone variation with respect to NM_000137.2
  267 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000137 unedited
  TTTTGTAATCCGACTTACTATAGNNGGCGGCCGCGCAATTCGGCACGAGGCCGCAGCCGT
  GCCGGGTGCTCTTCAGCATGTCCTTCATCCCGGTGGCCGAGGATTCCGACTTCCCCATCC
  ACAACCTGCCCTACGGCGTCTTCTCGACCAGAGGCGACCCAAGACCGAGGATAGGTGTGG
  CCATTGGCGACCAGATCCTGGACCTCAGCATCATCAAGCACCTCTTTACTGGTCCTGTCC
  TCTCCAAACACCAGGATGTCTTCAATCAGCCTACACTCAACAGCTTCATGGGCCTGGGTC
  AGGCTGCCTGGAAGGAGGCGAGAGTGTTCTTGCAGAACTTGCTCTCTGTGAGCCAAGCCA
  GGCTCAGAGATGACACCGAACTTCGGAAGTGTGCATTCATCTCCCAGGCTTCTGCCACGA
  TGCACCTTCCAGCCACCATAGGAGACTACACAGACTTCTATTCCTCTCGGCAGCATGCTA
  CCAACGTCGGAATCATGTTCAGGGACAAGGAGAATGCGTTGATGCCAAATTGGCTGCACT
  TACCAGTGGGCTACCATGGCCGTGCCTCCTCTGTCGTGGTGTCTGGCACCCCAATCCGAA
  GGCCCATGGGACAGATGAAACCTGATGACTCTAAGCCTCCCGTATATGGTGCCTGCAAGC
  TCCTTGGACATGGAGCTGGAAATGGCTTTTTTTGTACGCCCTGGAAACAGATTGGGAGAG
  CCGATCCCCATTTCCAAGGCCCATGAGCACATTCTTGGCATGGGCCCTCATGAACGATTG
  AAGTGCACGAGACATTCAGAAGTGNGAGTATGTCCCTCTCGGGCCCATCCTTGGGAAGAG
  CTTTGGGACCCCTGTCTCTCCGTGGGGGGGGCCCATGGTGCTCCTATGCACTTTTTGGGG
  CCAACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000137 unedited
  CCGCGGGCCGCATTTTAGNATCGAGTTTTTTTTTTTTTTTTTTGCGGCAGTGCAGGCCTC
  AAAAACAATGGCTTTATTTGTCACTGAGTGGCGGACCACGCCTACAGCCCGAGGGAGGAC
  CCCAGTTACAGGTTGAAACAAAGGCTTGAGCCCTTTGTTTCCAGAAGAGCAGAGAAAATC
  TCATGATGGCAGGAGAGCAGGCATCACTTTTCCAGCACACTGGCCAAAGCCGATGCGGTA
  ACCATCCCCCTGGCAGTACCCTGTTATGATGACTTTATCCCCGTTCAGCAGAAACTTCCT
  GGTCTGACCACTTCCCATGTCTATGGGCTTATTTCCCTTCCACGACCCCTCCTACCTGGT
  ACCCCAATCTCTCCGGCCTCCGCCACCTTCATGGCCCCACAAATCATCTCGCTTCCCTTA
  CCTTCGTCCACACACCGTCGACCCCTCCGGTACCGCCCTCTTCCTCCCGCTCCTCCTTTC
  TAAATCTCACCCAATATAAACCCCTATGCGTCTCTTTCCCCGTTTTTCCCGCTTCCCCCT
  CTCTACGCTCTTCCACCTCACCTCTTATCCTCCTTCCCCCCATTTTTTCCCGTCTTCTCC
  CCCTCTCTTCCCCTCCTTTTTTTTATCACCTTCACTAACTACTCCCCCCCTCCCTTCCTT
  CCTTATTTCTCGCTAATTCTTCCCCTCCCTCGCTTCCTTCCCCCCCTTTCCTTATTTTAT
  AATTCGTCTTTCTCCACCCGCTTCTTCCCCCCTCTTCCCGCCCCCCCCCCCCCACACCAT
  CGCACCCCTACATTTCCATTGTACCTTTCCACTTCCACCTTTTCTTATTTTCCCTTCCGC
  CCCTTTTCCTCTTTTTATTTTTTTCATCCTTCCTTTTCACTCACTTTCCATCTATTTTCC
  CTCTCCCCCCCTGTTATATTTACATCCTCATCCTCCCCTCCCCTTTCTCATTTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000137
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000137.1, NP_000128.1
• RefSeq Size: 1447 bp
• RefSeq ORF: 1260 bp
• Locus ID: 2184
• UniProt ID: P16930
• Cytogenetics: 15q25.1
• Domains: FAA_hydrolase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tyrosine metabolism
• Gene Summary: This gene encodes the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT). [provided by RefSeq, Jul 2008]