Fumarylacetoacetate hydrolase (FAH) (NM_000137) Human Tagged ORF Clone

CAT#: RC200388

FAH (Myc-DDK-tagged)-Human fumarylacetoacetate hydrolase (fumarylacetoacetase) (FAH)

Product Images

Circular map for RC200388

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  Western blot validation of overexpression lysate (Cat# LY400047) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200388 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FAH protein (Cat# TP300388). The protein was produced from HEK293T cells transfected with FAH cDNA clone (Cat# RC200388) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fumarylacetoacetate hydrolase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200388 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTCATCCCGGTGGCCGAGGATTCCGACTTCCCCATCCACAACCTGCCCTACGGCGTCTTCTCGA
  CCAGAGGCGACCCAAGACCGAGGATAGGTGTGGCCATTGGCGACCAGATCCTGGACCTCAGCATCATCAA
  GCACCTCTTTACTGGTCCTGTCCTCTCCAAACACCAGGATGTCTTCAATCAGCCTACACTCAACAGCTTC
  ATGGGCCTGGGTCAGGCTGCCTGGAAGGAGGCGAGAGTGTTCTTGCAGAACTTGCTGTCTGTGAGCCAAG
  CCAGGCTCAGAGATGACACCGAACTTCGGAAGTGTGCATTCATCTCCCAGGCTTCTGCCACGATGCACCT
  TCCAGCCACCATAGGAGACTACACAGACTTCTATTCCTCTCGGCAGCATGCTACCAACGTCGGAATCATG
  TTCAGGGACAAGGAGAATGCGTTGATGCCAAATTGGCTGCACTTACCAGTGGGCTACCATGGCCGTGCCT
  CCTCTGTCGTGGTGTCTGGCACCCCAATCCGAAGGCCCATGGGACAGATGAAACCTGATGACTCTAAGCC
  TCCCGTATATGGTGCCTGCAAGCTCTTGGACATGGAGCTGGAAATGGCTTTTTTTGTAGGCCCTGGAAAC
  AGATTGGGAGAGCCGATCCCCATTTCCAAGGCCCATGAGCACATTTTTGGAATGGTCCTTATGAACGACT
  GGAGTGCACGAGACATTCAGAAGTGGGAGTATGTCCCTCTCGGGCCATTCCTTGGGAAGAGTTTTGGGAC
  CACTGTCTCTCCGTGGGTGGTGCCCATGGATGCTCTCATGCCCTTTGCTGTGCCCAACCCGAAGCAGGAC
  CCCAGGCCCCTGCCGTATCTGTGCCATGACGAGCCCTACACATTTGACATCAACCTCTCTGTTAACCTGA
  AAGGAGAAGGAATGAGCCAGGCGGCTACCATATGCAAGTCCAATTTTAAGTACATGTACTGGACGATGCT
  GCAGCAGCTCACTCACCACTCTGTCAACGGCTGCAACCTGCGGCCGGGGGACCTCCTGGCTTCTGGGACC
  ATCAGCGGGCCGGAGCCAGAAAACTTCGGCTCCATGTTGGAACTGTCGTGGAAGGGAACGAAGCCCATAG
  ACCTGGGGAATGGTCAGACCAGGAAGTTTCTGCTGGACGGGGATGAAGTCATCATAACAGGGTACTGCCA
  GGGGGATGGTTACCGCATCGGCTTTGGCCAGTGTGCTGGAAAAGTGCTGCCTGCTCTCCTGCCATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200388 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFIPVAEDSDFPIHNLPYGVFSTRGDPRPRIGVAIGDQILDLSIIKHLFTGPVLSKHQDVFNQPTLNSF
  MGLGQAAWKEARVFLQNLLSVSQARLRDDTELRKCAFISQASATMHLPATIGDYTDFYSSRQHATNVGIM
  FRDKENALMPNWLHLPVGYHGRASSVVVSGTPIRRPMGQMKPDDSKPPVYGACKLLDMELEMAFFVGPGN
  RLGEPIPISKAHEHIFGMVLMNDWSARDIQKWEYVPLGPFLGKSFGTTVSPWVVPMDALMPFAVPNPKQD
  PRPLPYLCHDEPYTFDINLSVNLKGEGMSQAATICKSNFKYMYWTMLQQLTHHSVNGCNLRPGDLLASGT
  ISGPEPENFGSMLELSWKGTKPIDLGNGQTRKFLLDGDEVIITGYCQGDGYRIGFGQCAGKVLPALLPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000137
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000137.4
• RefSeq Size: 1810 bp
• RefSeq ORF: 1260 bp
• Locus ID: 2184
• UniProt ID: P16930
• Cytogenetics: 15q25.1
• Domains: FAA_hydrolase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tyrosine metabolism
• MW: 46.4 kDa
• Gene Summary: This gene encodes the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT). [provided by RefSeq, Jul 2008]