Iduronate 2 sulfatase (IDS) (NM_000202) Human Untagged Clone

CAT#: SC120050

IDS (untagged)-Human iduronate 2-sulfatase (IDS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Iduronate 2 sulfatase
• Synonyms: ID2S; MPS2; SIDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120050 sequence for NM_000202 edited (data generated by NextGen Sequencing)
  ATGCCGCCACCCCGGACCGGCCGAGGCCTTCTCTGGCTGGGTCTGGTTCTGAGCTCCGTC
  TGCGTCGCCCTCGGATCCGAAACGCAGGCCAACTCGACCACAGATGCTCTGAACGTTCTT
  CTCATCATCGTGGATGACCTGCGCCCCTCCCTGGGCTGTTATGGGGATAAGCTGGTGAGG
  TCCCCAAATATTGACCAACTGGCATCCCACAGCCTCCTCTTCCAGAATGCCTTTGCGCAG
  CAAGCAGTGTGCGCCCCGAGCCGCGTTTCTTTCCTCACTGGCAGGAGACCTGACACCACC
  CGCCTGTACGACTTCAACTCCTACTGGAGGGTGCACGCTGGAAACTTCTCCACCATCCCC
  CAGTACTTCAAGGAGAATGGCTATGTGACCATGTCGGTGGGAAAAGTCTTTCACCCTGGG
  ATATCTTCTAACCATACCGATGATTCTCCGTATAGCTGGTCTTTTCCACCTTATCATCCT
  TCCTCTGAGAAGTATGAAAACACTAAGACATGTCGAGGGCCAGATGGAGAACTCCATGCC
  AACCTGCTTTGCCCTGTGGATGTGCTGGATGTTCCCGAGGGCACCTTGCCTGACAAACAG
  AGCACTGAGCAAGCCATACAGTTGTTGGAAAAGATGAAAACGTCAGCCAGTCCTTTCTTC
  CTGGCCGTTGGGTATCATAAGCCACACATCCCCTTCAGATACCCCAAGGAATTTCAGAAG
  TTGTATCCCTTGGAGAACATCACCCTGGCCCCCGATCCCGAGGTCCCTGATGGCCTACCC
  CCTGTGGCCTACAACCCCTGGATGGACATCAGGCAACGGGAAGACGTCCAAGCCTTAAAC
  ATCAGTGTGCCGTATGGTCCAATTCCTGTGGACTTTCAGCGGAAAATCCGCCAGAGCTAC
  TTTGCCTCTGTGTCATATTTGGATACACAGGTCGGCCGCCTCTTGAGTGCTTTGGACGAT
  CTTCAGCTGGCCAACAGCACCATCATTGCATTTACCTCGGATCATGGGTGGGCTCTAGGT
  GAACATGGAGAATGGGCCAAATACAGCAATTTTGATGTTGCTACCCATGTTCCCCTGATA
  TTCTATGTTCCTGGAAGGACGGCTTCACTTCCGGAGGCAGGCGAGAAGCTTTTCCCTTAC
  CTCGACCCTTTTGATTCCGCCTCACAGTTGATGGAGCCAGGCAGGCAATCCATGGACCTT
  GTGGAACTTGTGTCTCTTTTTCCCACGCTGGCTGGACTTGCAGGACTGCAGGTTCCACCT
  CGCTGCCCCGTTCCTTCATTTCACGTTGAGCTGTGCAGAGAAGGCAAGAACCTTCTGAAG
  CATTTTCGATTCCGTGACTTGGAAGAGGATCCGTACCTCCCTGGTAATCCCCGTGAACTG
  ATTGCCTATAGCCAGTATCCCCGGCCTTCAGACATCCCTCAGTGGAATTCTGACAAGCCG
  AGTTTAAAAGATATAAAGATCATGGGCTATTCCATACGCACCATAGACTATAGGTATACT
  GTGTGGGTTGGCTTCAATCCTGATGAATTTCTAGCTAACTTTTCTGACATCCATGCAGGG
  GAACTGTATTTTGTGGATTCTGACCCATTGCAGGATCACAATATGTATAATGATTCCCAA
  GGTGGAGATCTTTTCCAGTTGTTGATGCCTTGA
  
  Clone variation with respect to NM_000202.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000202 unedited
  GTCAGGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATCGGCACGAGGGCGGGC
  CCGGGCGGCGGCTGTGTTGCGCAGTCTTCATGGGTTCCCGACGAGGAGGTCTCTGTGGCT
  GCGGCGGCGGCTGCTAACTGCGCCACCTGCTGCAGCCTGTCCCCGCCGCTCTGAAGCGGC
  CGCGTCGAAGCCGAAATGCCGCCACCCCGGACCGGCCGAGGCCTTCTCTGGCTGGGTCTG
  GTTCTGAGCTCCGTCTGCGTCGCCCTCGGATCCGAAACGCAGGCCAACTCGACCACAGAT
  GCTCTGAACGTTCTTCTCATCATCGTGGATGACCTGCGCCCCTCCCTGGGCTGTTATGGG
  GATAAGCTGGTGAGGTCCCCAAATATTGACCAACTGGCATCCCACAGCCTCCTCTTCCAG
  AATGCCTTTGCGCAGCAAGCAGTGTGCGCCCCGAGCCGCGTTTCTTTCCTCACTGGCAGG
  AGACCTGACACCACCCGCCTGTACGACTTCAACTCCTACTGGAGGGTGCACGCTGGAAAC
  TTCTCCACCATCCCCCAGTACTTCAAGGAGAATGGCTATGTGACCATGTCGGTGGGAAAA
  GTCTTTCACCCTGNGATATCTTCTAACCATACCGATGATTCTCCGTATAGCTGGTCTTTT
  CCACCTTATCATCCTTCCTCTGAGAAGTATGAAAACACTAAGACATGTCGAGGGCCAGAT
  GGAGAACTCCATGCCCACCCTGCTTGCCCTGTGGATGTGCTGGATGTTCCCGAGGGACCT
  TTGCCTGACAAACAGAGCACTTGAGCAGCCATACAGTTGTTGGGAAAGATGAAACGTCAG
  CCAGTCCTTTTCTTCCTGGGCCGTTGGTATCATAAGCCACACCATCCCCTTCAAATACCC
  CAAGGATTTCAGAAGTGTATCCCTTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000202 unedited
  ATGACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTAGATATTTAATA
  TTTATTTAGAAAGCTATTGACACTTCTGATGACTACTGATAACTGAGAAGATTCAACATG
  TTATAAATATGAGTTGTAAATATTCCATCCCTTTTGAGATTATATACACTAAGCTTTTTA
  CTACCACTGGCCTTTCTGGATACTTTCATATTTAGAGAATATAGAACTACATTCTGGGAG
  ATTGACAATGACCTAATATTACACTAATGAAAAGTCTTCACAGTCCTCAAAATAATTTAG
  AATTTAATGTTTTCTTTAATAAGCAATTAAAATGGAATATTTTCTGCCTCCTGAATAAAT
  ACATGTCTGTAACCAACCATCCCTAAAGAAGGATACTAATATATTTTGAGTGATTCAATC
  CACCCATGGATATAGTCACATCTTTAAATGTTTTCCAGTGTGAACAACAGTCTTTTAATA
  GATGGCCATTGAGTTCAAGGGTCATATTATTTGCTAACAAATTATTATTAAATTATAAAC
  AAAATAATCAGGAACTTAGTGTGGTGCCTAGTTTGATATATGATTACTTTTTGAAATGCA
  CTAAATTCCACAAATAATGAAAGTATTCTTTGTGTATAATGTTATGTTTGGTTATTATGT
  ATGGTCTTCGTATCCAAAGGTATGACATAACTTGAGTTTGTTTGCTTTGTATTTATTCAG
  TAAATAAGCCGTAACTGTTTTAAAAAGAGGGAAATTAAAAAAAAACTGGTCCAATTACCA
  ATTATAAATTTTAATAAAGACTAAACGAANAGGNNTTGGCTGNTACATATTNCTCAGGCC
  AAAAATGTGATGCATGTTTGGATTAACTAGCCCTNNCAGCTGCTNCATATTATGGNNTAT
  CACAAAACGACAGCTTTAAACTCTNCTCTTACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000202
• Insert Size: 2830 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000202.2, NP_000193.1
• RefSeq Size: 2504 bp
• RefSeq ORF: 1653 bp
• Locus ID: 3423
• UniProt ID: P22304
• Cytogenetics: Xq28
• Domains: Sulfatase
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Gene Summary: This gene encodes a member of the sulfatase family of proteins. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. This enzyme is involved in the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.