Heparin Cofactor II (SERPIND1) (NM_000185) Human Untagged Clone

CAT#: SC120039

SERPIND1 (untagged)-Human serpin peptidase inhibitor, clade D (heparin cofactor), member 1 (SERPIND1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Heparin Cofactor II
• Synonyms: D22S673; HC2; HCF2; HCII; HLS2; LS2; THPH10
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120039 sequence for NM_000185 edited (data generated by NextGen Sequencing)
  ATGAAACACTCATTAAACGCACTTCTCATTTTCCTCATCATAACATCTGCGTGGGGTGGG
  AGCAAAGGCCCGCTGGATCAGCTAGAGAAAGGAGGGGAAACTGCTCAGTCTGCAGATCCC
  CAGTGGGAGCAGTTAAATAACAAAAACCTGAGCATGCCTCTTCTCCCTGCCGACTTCCAC
  AAGGAAAACACCGTCACCAACGACTGGATTCCAGAGGGGGAGGAGGACGACGACTATCTG
  GACCTGGAGAAGATATTCAGTGAAGACGACGACTACATCGACATCGTCGACAGTCTGTCA
  GTTTCCCCGACAGACTCTGATGTGAGTGCTGGGAACATCCTCCAGCTTTTTCATGGCAAG
  AGCCGGATCCAGCGTCTTAACATCCTCAACGCCAAGTTCGCTTTCAACCTCTACCGAGTG
  CTGAAAGACCAGGTCAACACTTTCGATAACATCTTCATAGCACCCGTTGGCATTTCTACT
  GCGATGGGTATGATTTCCTTAGGTCTGAAGGGAGAGACCCATGAACAAGTGCACTCGATT
  TTGCATTTTAAAGACTTTGTTAATGCCAGCAGCAAGTATGAAATCACGACCATTCATAAT
  CTCTTCCGTAAGCTGACTCATCGCCTCTTCAGGAGGAATTTTGGGTACACACTGCGGTCA
  GTCAATGACCTTTATATCCAGAAGCAGTTTCCAATCCTGCTTGACTTCAAAACTAAAGTA
  AGAGAGTATTACTTTGCTGAGGCCCAGATAGCTGACTTCTCAGACCCTGCCTTCATATCA
  AAAACCAACAACCACATCATGAAGCTCACCAAGGGCCTCATAAAAGATGCTCTGGAGAAT
  ATAGACCCTGCTACCCAGATGATGATTCTCAACTGCATCTACTTCAAAGGATCCTGGGTG
  AATAAATTCCCAGTGGAAATGACACACAACCACAACTTCCGGCTGAATGAGAGAGAGGTA
  GTTAAGGTTTCCATGATGCAGACCAAGGGGAACTTCCTCGCAGCAAATGACCAGGAGCTG
  GACTGCGACATCCTCCAGCTGGAATACGTGGGGGGCATCAGCATGCTAATTGTGGTCCCA
  CACAAGATGTCTGGGATGAAGACCCTCGAAGCGCAACTGACACCCCGGGTGGTGGAGAGA
  TGGCAAAAAAGCATGACAAACAGAACTCGAGAAGTGCTTCTGCCGAAATTCAAGCTGGAG
  AAGAACTACAATCTAGTGGAGTCCCTGAAGTTGATGGGGATCAGGATGCTGTTTGACAAA
  AATGGCAACATGGCAGGCATCTCAGACCAAAGGATCGCCATCGACCTGTTCAAGCACCAA
  GGCACGATCACAGTGAACGAGGAAGGCACCCAAGCCACCACTGTGACCACGGTGGGGTTC
  ATGCCGCTGTCCACCCAAGTCCGCTTCACTGTCGACCGCCCCTTTCTTTTCCTCATCTAC
  GAGCACCGCACCAGCTGCCTGCTCTTCATGGGAAGAGTGGCCAACCCCAGCAGGTCCTAG
  
  Clone variation with respect to NM_000185.3
  1446 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000185 unedited
  TCAGGAATTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACCAGGCCACTTC
  TCAGAACACAGAGCTTTAGCTCCGCCAAAATGAAACACTCATTAAACGCACTTCTCATTT
  TCCTCATCATAACATCTGCGTGGGGTGGGAGCAAAGGCCCGCTGGATCAGCTAGAGAAAG
  GAGGGGAAACTGCTCAGTCTGCAGATCCCCAGTGGGAGCAGTTAAATAACAAAAACCTGA
  GCATGCCTCTTCTCCCTGCCGACTTCCACAAGGAAAACACCGTCACCAACGACTGGATTC
  CAGAGGGGGAGGAGGACGACGACTATCTGGACCTGGAGAAGATATTCAGTGAAGACGACG
  ACTACATCGACATCGTCGACAGTCTGTCAGTTTCCCCGACAGACTCTGATGTGAGTGCTG
  GGAACATCCTCCAGCTTTTTCATGGCAAGAGCCGGATCCAGCGTCTTAACATCCTCAACG
  CCAAGTTCGCTTTCAACCTCTACCGAGTGCTGAAAGACCAGGTCAACACTTTCGATAACA
  TCTTCATAGCACCCGTTGGCATTTCTACTGCGATGGGTATGATTTCCTTAGGTCTGAAGG
  GAGAGACCCATGAACAAGTGCACTCGATTTTGCATTTTAAAGACTTTGTTAATGCCAGCA
  GCAAGTATGAAATCACGACCATTCATAATCTCTTCCGTAAGCTGACTCATCGCCTCTTCA
  GGAGGAATTTTGGGTACACACTGCGGTCAGTCAATGACCTTTATATCCAGAAGCAGTTTC
  CAATCCTGCTTGACTTCAAACTAAGTAAGAGAGTATTACTNTGCTGAGGCCCAATAGCTG
  ACTTCTCAGACCTGCCTTCTATCAAAACCACACCACATCTGAGCTCACAGGGCCTCTANA
  GAGCTCTGAGATATAGACCTGCTACCAGAGATGATCTCACTGCTCACTTCAAGAACCTGG
  TGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000185 unedited
  TTACTTTGNACCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTAAAT
  GTGGCTACATTTATTTATCTCTAGTATGGGAGACATGGCACACACTGTCCTCCAGGTCAC
  AGGGAGGCAACAGCAGGAGGGACTGATGGCATTCATCATGGATTTGAAAGGTCAGGCTTT
  GAGAAAATTAAGCCCTAACATTAGCCCAGGGCACCCCTCATATGTCTTGAGGTGGCTTCT
  TCCCTGGACATCAAGAATGGTGCTAGTTGTGTACCCTCCTTGGGAGAGGTAAGGAATTAG
  TCACACCACTCATTTCTGTGCTTGGTATTCAGATGAGGGGCCTGGTGGAGAAAGGCTTGA
  GTGACAGAGTCAGGTGTGCAAGGATGGGGTCACCGGAGCGGGAGGTGCTTAGGACGCGCT
  GCTCAGGTTTGCTGTGAAGAGTGGGGAGGTGAGACCCTCTAGGTAACAGCAGACACACTA
  CAGTTACAGTGACTTCTATGAGCTTACAGCATGGGCTATTGTGCAGAATTGATTCCAACA
  AGCCTCTCTTCTTGGTCGTTTCTAAGCTCCTCTCATATGGGCCTCGAATTCAGATTGGTA
  GCGTAAACTACGTAAATGATGCCAGAACATCTCTGTTCTCGTTGTTGGAATGGAAACAAA
  ATGAGGGTGCCCCCAAGGCACTTCAGACACCTAGACCTCCACCTCTAGGACCTGCTGGGG
  GNTGGCCACTCTTCCCATGAAGAGCANGCAGCTGGTGCGGTGCTCGTAAATGAAGAAAAG
  AAAGGGGCGGTCGACAGTGAAAGCGACTTNGGGTGGNACAGCGCATGAACCCCACCGTGG
  TCACAGTGGGTGCCTGGGGTGCCNTTCTCGTTCACTGNGATCGTGCCTTGGTGCNTTGAC
  AGNTCGATGGCGATCCTTTTGTCTGAGATGCCTGCATGTTGCCATTTTTGNCAAACACAT
  CCTGATCCCATCACTTCGGGACTCACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000185
• Insert Size: 2290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000185.3, NP_000176.2
• RefSeq Size: 2237 bp
• RefSeq ORF: 1500 bp
• Locus ID: 3053
• UniProt ID: P05546
• Cytogenetics: 22q11.21
• Domains: SERPIN
• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2015]