TAT (NM_000353) Human Untagged Clone

CAT#: SC119924

TAT (untagged)-Human tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAT
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119924 sequence for NM_000353 edited (data generated by NextGen Sequencing)
  ATGGACCCATACATGATTCAGATGAGCAGCAAAGGCAACCTCCCCTCAATTCTGGACGTG
  CATGTCAACGTTGGTGGGAGAAGCTCTGTGCCGGGAAAAATGAAAGGCAGAAAGGCCAGG
  TGGTCTGTGAGGCCCTCAGACATGGCCAAGAAAACTTTCAACCCCATCCGAGCCATTGTG
  GACAACATGAAGGTGAAACCAAATCCAAACAAAACCATGATTTCCCTGTCCATTGGGGAC
  CCTACTGTGTTTGGAAACCTGCCTACAGACCCTGAAGTTACCCAGGCAATGAAAGATGCC
  CTGGACTCGGGCAAATATAATGGCTATGCCCCATCCATCGGCTTCCTATCCAGTCGGGAG
  GAGATTGCTTCTTATTACCACTGTCCTGAGGCACCCCTAGAAGCTAAGGACGTCATTCTG
  ACAAGTGGCTGCAGCCAAGCTATTGACCTTTGTTTAGCTGTGTTGGCCAACCCAGGGCAA
  AACATCCTGGTTCCAAGACCTGGTTTCTCTCTCTACAAGACTCTGGCTGAGTCTATGGGA
  ATTGAGGTCAAACTCTACAATTTGTTGCCAGAGAAATCTTGGGAAATTGACCTGAAACAA
  CTGGAATATCTAATTGATGAAAAGACAGCTTGTCTCATTGTCAATAATCCATCAAACCCC
  TGTGGGTCAGTGTTCAGCAAACGTCATCTTCAGAAGATTCTGGCAGTGGCTGCACGGCAG
  TGTGTCCCCATCTTAGCTGATGAGATCTATGGAGACATGGTGTTTTCGGATTGCAAATAT
  GAACCACTGGCCACCCTCAGCACCGATGTCCCCATCCTGTCCTGTGGAGGGCTGGCCAAG
  CGCTGGCTGGTTCCTGGCTGGAGGTTGGGCTGGATCCTCATTCATGACCGAAGAGACATT
  TTTGGCAATGAGATCCGAGATGGGCTGGTGAAGCTGAGTCAGCGCATTTTGGGACCCTGT
  ACCATTGTCCAGGGAGCTCTGAAAAGCATCCTATGTCGCACCCCGGGAGAGTTTTACCAC
  AACACTCTGAGCTTCCTCAAGTCCAATGCTGATCTCTGTTATGGGGCGTTGGCTGCCATC
  CCTGGACTCCGGCCAGTCCGCCCTTCTGGGGCTATGTACCTCATGGTTGGAATTGAGATG
  GAACATTTCCCAGAATTTGAGAACGATGTGGAGTTCACGGAGCGGTTAGTTGCTGAGCAG
  TCTGTCCACTGCCTCCCAGCAACGTGCTTTGAGTACCCGAATTTCATCCGAGTGGTCATC
  ACAGTCCCCGAGGTGATGATGCTGGAGGCGTGCAGCCGGATCCAGGAGTTCTGTGAGCAG
  CACTACCATTGTGCTGAAGGCAGCCAGGAGGAGTGTGATAAATAG
  
  Clone variation with respect to NM_000353.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000353 unedited
  GGGGTCAGATTTTGTAAACGACTCATATAGGCGGCCGCGNAATTCGCACGAGCAAANAAA
  AGCTAAGGGAGCTTTCGGGGTTGGCTTCTTGGAGGCTGCTTTCTCCTTTACTTGGAAGGC
  TTCGCTAGTGATGGACCCATACATGATTCAGATGAGCAGCAAAGGCAACCTCCCCTCAAT
  TCTGGACGTGCATGTCAACGTTGGTGGGAGAAGCTCTGTGCCGGGAAAAATGAAAGGCAG
  AAAGGCCAGGTGGTCTGTGAGGCCCTCAGACATGGCCAAGAAAACTTTCAACCCCATCCG
  AGCCATTGTGGACAACATGAAGGTGAAACCAAATCCAAACAAAACCATGATTTCCCTGTC
  CATTGGGGACCCTACTGTGTTTGGAAACCTGCCTACAGACCCTGAAGTTACCCAGGCAAT
  GAAAGATGCCCTGGACTCGGGCAAATATAATGGCTATGCCCCATCCATCGGCTTCCTATC
  CAGTCGGGAGGAGATTGCTTCTTATTACCACTGTCCTGAGGCACCCCTAGAAGCTAAGGA
  CGTCATTCTGACAAGTGGCTGCAGCCAAGCTATTGACCTTTGTTTAGCTGTGTTGGCCAA
  CCCAGGGCAAAACATCCTGGTTCCAAGACCTGGTTTCTCTCTCTACAAGACTCTGGCTGA
  GTCTATGGGAATTGAGGTCAAACTCTACAATTTGTTGCCAGAGAAATCTTGNGAAATTGA
  CCTGAAACAACTGGAATATCTAATTGATGAAAAGACAGCTTGTCTCATTGTCAATAATCC
  ATCANACCCCTGTGGGTCAGTGTTCAGCAAACGTCATCTTCAGAAGATCTGGCAGTGGCT
  GCACGGCAGTGTGTCCCCATCTTAGCTGATGAGATCTATGGAGACATGGTGTTTNTCGAT
  TGCAAATATGACCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000353 unedited
  CCCCCAATTCGTGTGNACCGCGGCCCGCAATTCTAGACNCGGCTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTACAAAGTTGAGTAATACTTTATTTGAAAAAATGAAAAGTGCACACACAC
  ACACACACACTTACATAGGCACAGGATAATCTGGAAGTATGACCAGCAAATGATAACTGA
  TTCCCTCAGGGCAGAACAAACTGGGTGGCTGACGGACCGGAATGAGAAACAAGGACAGTT
  GCGTTTGTTTGTATCGTTTGAAATTGCCCAGTGTGTATGTGTTCTTTTCAAATGTTTGAA
  GAACCATTGGCTCCCTTATCAAAATGTAAACACCAGGGAAAACCAGGAATTCAAGAAGCC
  TACCAATTCTGTAATGCTGGGTACAATTTTCTGCACGTGTACAACTAAAAGGCCCTTTGT
  AAATCACTGAGCCTTCCTGTTTATTTCTGGTTACGAGATCTTTGGGGGCTTGGATGGAGG
  AACAGGAAAAGAGGAGCCAGCCTTTTTTGGAAAATTGGCGCCAGCGGCGGCATACGGCAA
  ACTGAAACCTTTATCTAACCTCATCCTTTGTATATTCGCCTTGGATTTGCTGAAAAAAAA
  TAAAAGGCAAACTTTGCTGTGAGCTCACTGACCTCCCCTTGAACGGGACACCTATGCTGT
  TTATTTTTGTGACAGGACATTAATTGCCTGTGACTGCCACGGCTGCACACCTGAAACTCC
  CTCTTTTACATAAGGAAAATTCCCCTTGGTGCGACATCTTTCCCCTCGTTCTGAAAGAAT
  TCTTACCCCTCCCCCTCTGACAGGTACACTCAAGGATCCCTTCTCTAAGGGAAGGCTTTA
  CCAAAAAATTCAAACCAGCCATTGTGCTCACGACGACTTGCGTCAACAAATAACTCCCCC
  AACTCCTTTGCCCTTTTTCACTCCCTGTTCAGGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000353
• Insert Size: 3860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000353.1, NP_000344.1
• RefSeq Size: 2754 bp
• RefSeq ORF: 1365 bp
• Locus ID: 6898
• UniProt ID: P17735
• Cytogenetics: 16q22.2
• Domains: aminotran_1_2
• Protein Families: Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism, Ubiquinone and other terpenoid-quinone biosynthesis
• Gene Summary: This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible cognitive disability. A regulator gene for tyrosine aminotransferase is X-linked. [provided by RefSeq, Jul 2008]