CD42c (GP1BB) (NM_000407) Human Untagged Clone

CAT#: SC119912

GP1BB (untagged)-Human glycoprotein Ib (platelet), beta polypeptide (GP1BB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD42c
• Synonyms: BDPLT1; BS; CD42C; GPIBB; GPIbbeta
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119912 sequence for NM_000407 edited (data generated by NextGen Sequencing)
  ATGGGCTCCGGGCCGCGCGGGGCGCTGAGCTTACTGCTCCTGCTGCTGGCCCCGCCGAGC
  CGCCCGGCCGCAGGTTGCCCGGCGCCCTGTAGCTGCGCGGGGACGCTCGTGGACTGCGGG
  CGCCGCGGGCTGACTTGGGCCTCGCTGCCGACCGCCTTCCCTGTCGACACAACCGAGCTG
  GTGCTGACCGGCAACAACCTGACGGCGCTGCCGCCGGGGCTGCTGGACGCGCTGCCCGCG
  CTGCGCACCGCACACCTGGGCGCCAACCCCTGGCGCTGCGACTGCCGCCTTGTGCCGCTG
  CGCGCCTGGCTGGCCGGCCGCCCCGAGCGTGCGCCCTACCGCGACCTGCGTTGCGTGGCG
  CCCCCAGCGCTGCGCGGCCGCCTGCTGCCCTATCTGGCCGAGGACGAGCTGCGCGCCGCT
  TGCGCTCCCGGCCCGCTCTGCTGGGGGGCGCTGGCGGCGCAGCTTGCGCTGCTGGGCCTT
  GGGCTGCTGCACGCGTTGCTGCTGGTGCTGCTGCTGTGCCGCCTGCGGAGGCTGCGGGCC
  CGGGCCCGCGCTCGCGCCGCAGCCCGGCTGTCGCTGACCGACCCGCTGGTGGCCGAGCGA
  GCCGGAACCGACGAGTCCTGA
  
  Clone variation with respect to NM_000407.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000407 unedited
  NAGTTCATTTTTGTAATAGACTCACTATAGGCGGCCGCGAATTCGGCACCAGGGCCTCCC
  GATGTTCCCACCCGCATGATCCCTTCCCGCCACACGATGCTCCGTTTTCTTCCGTTGTGA
  ATGCCGCGTCCTGTCCTGGTGACAGGAGAACAATGTTGGTGAACGTCGCAGCGGGTGTCC
  GAGTGCTCCGTGTGCCCCTGAGAGCGGGTGGGAGCGGAAGCCTGAGCGGCCTGCGGCCTC
  CGGCGATAGTGTGCTATCTGCCGCTGCAGCGCGCGTCCGCGCGGCCTCTGGGCTATTTCT
  GGCCAGGCCGCAGCACTGTGGTCGGTGCGGGCGTGGCAGGGGCGGGGCGGCCTTATCGCT
  CGGCTCTCCCGCCTACGCCTCCCGCTGCAGAGTAAGCCGGGCTGCCGTCTTCTCGCCATG
  GGCTCCGGGCCGCGCGGGGCGCTGAGCTTACTGCTCCTGCTGCTGGCCCCGCCGAGCCGC
  CCGGCCGCAGGTTGCCCGGCGCCCTGTAGCTGCGCGGGGACGCTCGTGGACTGCGGGCGC
  CGCGGGCTGACTTGGGCCTCGCTGCCGACCGCCTTCCCTGTCGACACAACCGAGCTGGTG
  CTGACCGGCAACAACCTGACGGCGCTGCCGCCGGGGCTGCTGGACGCGCTGCCCGCGCTG
  CGCACCGCACACCTGGGCGCCAACCCCTGGCGCTGCGACTGCCGCCTTGTGCCGCTGCGC
  CCCTGGCTGGCCGGCCGCCCCGAGCGTGCGCCCTACCGCACCTGGGTTTGCGTGGCGCCC
  CCAGCGCTGCGCGGCCGCCTGCTGCCCTATCTGGGCCCGAGACGAGCTGCGCCGCCGCTT
  GCGCNTCCGGCCGCTCTGCTTGGGNGGCGCTTGCGGGCGCAACTTGCGCTGCTGGGCCTT
  GGGCTGCTGCACGCGTTGCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000407 unedited
  NTTGACTCTGANACCGCGGCCGCATACTAAGATCAAGTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTCCAAATCAGAAGGGTTTATTCACCACCAAAGTTTGGAGAGGGAGACC
  CAGCAGGGTCTGGACCGCTATTGGGCCCCATGGTCCTCCCAACCCTGGAAGTGCGGGTTC
  GCCTCCCTCGGGGTAGGGGGGGCAGGATTTCTGTCCCACGTGGTCTGGGCCTGCAGGGGG
  GGGGGGTCTGAAAAATGGGCAGCGGAGGAGGCGGGGACCGGTCCAGGTTGGCGCGAGGGC
  CCCTCGGGCAGGGTCCTGTCAAGTTTGCAGGCCCGTGTTGCCCACCTCCGGTTTTTTCCT
  CAGGACCGCCCTCTTGTCTCTCTCCGCTTGTGTTCCCCTCTCCCCCTTTCTTCCCCCCCC
  CCCCCCCACTCTTGCTCCCCCTTCCCCCGCCCTTAAATCTGTCCCCGCCCCTCCATTTTT
  CCACCCACTCCTCATTGCGCGCACTTCCCCCTTCCCCCTCCCCTCGCTCCTCACCCCCTA
  TTAATTTTTCATTGTGTTTCGCATGTTCTGTTCCCCCCCCCCATTTTCTCTTCCACCTTT
  TTCCCCCCCCCTTTCTGTTCCCCTTCCCTTCCACGCCCCGTCCTCCCCTTTACCCCCCCT
  TCGCCTCCCCCCCCATCCCTCCCTTTTCTTCTCTTTTCTGCCTTGCTTTTTCCTTGTTAT
  TGCCTTCCATTACTTAGCTTGTTCGTCCTTCTTCTTTTTCTCCCCTTTTTTCCCTCTCCT
  TATATTCTTTTTTTCTTATCACTAATTCTCCCTACCTCCTTTGCTCACTCTCCTTTCTCC
  CTCTGTTCCGCCCCTCTCCCCCCCTCTCGTTTTCGTTCTCCTTCCCTCCCATTCCCTGTA
  CCACCATGCCTTCTATCCCCAACCTCGCCTTCCCTTCTTCACTTCTCTCCTCCCCATAAC
  CCATCTTCCCATTTACTCACTACTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000407
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000407.3, NP_000398.1
• RefSeq Size: 955 bp
• RefSeq ORF: 621 bp
• Locus ID: 2812
• UniProt ID: P13224
• Cytogenetics: 22q11.21
• Domains: LRRNT, LRRCT, LRR
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ECM-receptor interaction, Hematopoietic cell lineage
• Gene Summary: Platelet glycoprotein Ib (GPIb) is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. It is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (VWF), and mediates platelet adhesion in the arterial circulation. GPIb alpha chain provides the VWF binding site, and GPIb beta contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. Mutations in the GPIb beta subunit have been associated with Bernard-Soulier syndrome, velocardiofacial syndrome and giant platelet disorder. The 206 amino acid precursor of GPIb beta is synthesized from a 1.0 kb mRNA expressed in plateletes and megakaryocytes. A 411 amino acid protein arising from a longer, unspliced transcript in endothelial cells has been described; however, the authenticity of this product has been questioned. Yet another less abundant GPIb beta mRNA species of 3.5 kb, expressed in nonhematopoietic tissues such as endothelium, brain and heart, was shown to result from inefficient usage of a non-consensus polyA signal in the neighboring upstream gene (SEPT5, septin 5). In the absence of polyadenylation from its own imperfect site, the SEPT5 gene produces read-through transcripts that use the consensus polyA signal of this gene. [provided by RefSeq, Dec 2010]