IGF2 (NM_000612) Human Untagged Clone

CAT#: SC119786

IGF2 (untagged)-Human insulin-like growth factor 2 (somatomedin A) (IGF2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IGF2
• Synonyms: C11orf43; GRDF; IGF-II; PP9974; SRS3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000612 edited
  GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGTCGGCCCCCGCGACTCGGCCA
  GAGCGGCGCTGGCAGAGGAGTGTCCGGCAGGAGGGCCAACGCCCGCTGTTCGGTTTGCGA
  CACGCAGCAGGGAGGTGGGCGGCAGCGTCGCCGGCTTCCAGACACCAATGGGAATCCCAA
  TGGGGAAGTCGATGCTGGTGCTTCTCACCTTCTTGGCCTTCGCCTCGTGCTGCATTGCTG
  CTTACCGCCCCAGTGAGACCCTGTGCGGCGGGGAGCTGGTGGACACCCTCCAGTTCGTCT
  GTGGGGACCGCGGCTTCTACTTCAGCAGGCCCGCAAGCCGTGTGAGCCGTCGCAGCCGTG
  GCATCGTTGAGGAGTGCTGTTTCCGCAGCTGTGACCTGGCCCTCCTGGAGACGTACTGTG
  CTACCCCCGCCAAGTCCGAGAGGGACGTGTCGACCCCTCCGACCGTGCTTCCGGACAACT
  TCCCCAGATACCCCGTGGGCAAGTTCTTCCAATATGACACCTGGAAGCAGTCCACCCAGC
  GCCTGCGCAGGGGCCTGCCTGCCCTCCTGCGTGCCCGCCGGGGTCACGTGCTCGCCAAGG
  AGCTCGAGGCGTTCAGGGAGGCCAAACGTCACCGTCCCCTGATTGCTCTACCCACCCAAG
  ACCCCGCCCACGGGGGCGCCCCCCCAGAGATGGCCAGCAATCGGAAGTGAGCAAAACTGC
  CGCAAGTCTGCAGCCCGGCGCCACCATCCTGCAGCCTCCTCCTGACCACGGACGTTTCCA
  TCAGGTTCCATCCCGAAAATCTCTCGGTTCCACGTCCCCCTGGGGCTTCTCCTGACCCAG
  TCCCCGTGCCCCGCCTCCCCGAAACAGGCTACTCTCCTCGGCCCCCTCCATCGGGCTGAG
  GAAGCACAGCAGCATCTTCAAACATGTACAAAATCGATTGGCTTTAAACACCCTTCACAT
  ACCCTCCCCCCAAATTATCCCCAATTATCCCCACACATAAAAAATCAAAACATTAAACTA
  ACCCCCTTCCCCCCCCCCCACAACAACCCTCTTAAAACTAATTGGCTTTTTAGAAACACC
  CCACAAAAGCTCAGAAATTGGCTTTAAAAAAAACAACCACCAAAAAAAATCAATTGGCTA
  AAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000612 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAAT
  TCGGCACGAGGTCGGCCCCCGCGACTCGGCCAGAGCGGCGCTGGCAGAGGAGTGTCCGGC
  AGGAGGGCCAACGCCCGCTGTTCGGTTTGCGACACGCAGCAGGGAGGTGGGCGGCAGCGT
  CGCCGGCTTCCAGACACCAATGGGAATCCCAATGGGGAAGTCGATGCTGGTGCTTCTCAC
  CTTCTTGGCCTTCGCCTCGTGCTGCATTGCTGCTTACCGCCCCAGTGAGACCCTGTGCGG
  CGGGGAGCTGGTGGACACCCTCCAGTTCGTCTGTGGGGACCGCGGCTTCTACTTCAGCAG
  GCCCGCAAGCCGTGTGAGCCGTCGCAGCCGTGGCATCGTTGAGGAGTGCTGTTTCCGCAG
  CTGTGACCTGGCCCTCCTGGAGACGTACTGTGCTACCCCCGCCAAGTCCGAGAGGGACGT
  GTCGACCCCTCCGACCGTGCTTCCGGACAACTTCCCCAGATACCCCGTGGGCAAGTTCTT
  CCAATATGACACCTGNAAGCAGTCCACCCAGCGCCTGCGCAGGGGCCTGCCTGCCCTCCT
  GCGTGCCCGCCGGNGTCACGTGCTCGCCAAGAGCTCGAGGCGTTCAGGGAGGCCAAACGT
  CACCGTCCCCTGATTGCTCTACCCACCCAAGACCCCGCCCACGGGGGCGCCCCCCCAGAG
  ATGGCCAGCATTCGGAAGTGAGCAAAACTGGCCGCAGTCTGCAGCCCGGCGCCACCATCC
  TGCAGCCTCCTNCTGACCACGNACGTTTCCATCANGTTCCATCCCGAATCTCTGGNNNTC
  CACGTCCCCTGGGGCTTCTCTTGAACCAATNCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000612
• Insert Size: 1210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000612.1, NP_000603.1
• RefSeq Size: 1640 bp
• RefSeq ORF: 543 bp
• Locus ID: 3481
• UniProt ID: P01344
• Cytogenetics: 11p15.5
• Domains: IlGF
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Gene Summary: This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
  Transcript Variant: This variant (1) represents the most predominant transcript. Variants 1, 2, 4 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.