BCKDHA (NM_000709) Human Untagged Clone

CAT#: SC119718

BCKDHA (untagged)-Human branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BCKDHA
• Synonyms: BCKDE1A; MSU; MSUD1; OVD1A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000709, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGTAGCGATCGCTGCAGCGAGGGTCTGGCGGCTAAACCGTGGTTTGAGCCAGGCTGCCCTCCTGC
  TGCTGCGGCAGCCTGGGGCTCGGGGACTGGCTAGATCTCACCCCCCCAGGCAGCAGCAGCAGTTTTCATC
  TCTGGATGACAAGCCCCAGTTCCCAGGGGCCTCGGCGGAGTTTATAGATAAGTTGGAATTCATCCAGCCC
  AACGTCATCTCTGGAATCCCCATCTACCGCGTCATGGACCGGCAAGGCCAGATCATCAACCCCAGCGAGG
  ACCCCCACCTGCCGAAGGAGAAGGTGCTGAAGCTCTACAAGAGCATGACACTGCTTAACACCATGGACCG
  CATCCTCTATGAGTCTCAGCGGCAGGGCCGGATCTCCTTCTACATGACCAACTATGGTGAGGAGGGCACG
  CACGTGGGGAGTGCCGCCGCCCTGGACAACACGGACCTGGTGTTTGGCCAGTACCGGGAGGCAGGTGTGC
  TGATGTATCGGGACTACCCCCTGGAACTATTCATGGCCCAGTGCTATGGCAACATCAGTGACTTGGGCAA
  GGGGCGCCAGATGCCTGTCCACTACGGCTGCAAGGAACGCCACTTCGTCACTATCTCCTCTCCACTGGCC
  ACGCAGATCCCTCAGGCGGTGGGGGCGGCGTACGCAGCCAAGCGGGCCAATGCCAACAGGGTCGTCATCT
  GTTACTTCGGCGAGGGGGCAGCCAGTGAGGGGGACGCCCATGCCGGCTTCAACTTCGCTGCCACACTTGA
  GTGCCCCATCATCTTCTTCTGCCGGAACAATGGCTACGCCATCTCCACGCCCACCTCTGAGCAGTATCGC
  GGCGATGGCATTGCAGCACGAGGCCCCGGGTATGGCATCATGTCAATCCGCGTGGATGGTAATGATGTGT
  TTGCCGTATACAACGCCACAAAGGAGGCCCGACGGCGGGCTGTGGCAGAGAACCAGCCCTTCCTCATCGA
  GGCCATGACCTACAGGATCGGGCACCACAGCACCAGTGACGACAGTTCAGCGTACCGCTCGGTGGATGAG
  GTCAATTACTGGGATAAACAGGACCACCCCATCTCCCGGCTGCGGCACTATCTGCTGAGCCAAGGCTGGT
  GGGATGAGGAGCAGGAGAAGGCCTGGAGGAAGCAGTCCCGCAGGAAGGTGATGGAGGCCTTTGAGCAGGC
  CGAGCGGAAGCCCAAACCCAACCCCAACCTACTCTTCTCAGACGTGTATCAGGAGATGCCCGCCCAGCTC
  CGCAAGCAGCAGGAGTCTCTGGCCCGCCACCTGCAGACCTACGGGGAGCACTACCCACTGGATCACTTCG
  ATAAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000709 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTAGCGATCGCTGCAGCGA
  GGGTCTGGCGGCTAAACCGTGGTTTGAGCCAGGCTGCCCTCCTGCTGCTGCGGCAGCCTG
  GGGCTCGGGGACTGGCTAGATCTCACCCCCCCAGGCAGCAGCAGCAGTTTTCATCTCTGG
  ATGACAAGCCCCAGTTCCCAGGGGCCTCGGCGGAGTTTATAGATAAGTTGGAATTCATCC
  AGCCCAACGTCATCTCTGGAATCCCCATCTACCGCGTCATGGACCGGCAAGGCCAGATCA
  TCAACCCCAGCGAGGACCCCCACCTGCCGAAGGAGAAGGTGCTGAAGCTCTACAAGAGCA
  TGACACTGCTTAACACCATGGACCGCATCCTCTATGAGTCTCAGCGGCAGGGCCGGATCT
  CCTTCTACATGACCAACTATGGTGAGGAGGGCACGCACGTGGGGAGTGCCGCCGCCCTGG
  ACAACACGGACCTGGTGTTTGGCCAGTACCGGGAGGCAGGTGTGCTGATGTATCGGGACT
  ACCCCCTGGAACTATTCATGGCCCAGTGCTATGGCAACATCAGTGACTTGGGCAAGGGGC
  GCCAGATGCCTGTCCACTACGGCTGNCAGGAACGCCACTTCGTCACTATCTCCTCTCCAC
  TGGCCACGCAGATCCCTCAGGCGGGTGGGGGCGGCGTACGCAGCCAAGCGGGCCAATGCC
  AACAGGGNTCGTCATCTGGTACTTCNGCGAGGGGGCAGCCAGTGAGGGGGACGCCATGCC
  NGCTTNCACTTCGCTGCCACACTTGAGTGCCCCATCATCTTCTTCTTGCCGGACAAGGGN
  TACGCCATCTCCACGCCCACCTCTGAGCAGTATCGCGGCGATGGCATTGCAGCACGAGGC
  CCCCGGTATGGCATCATGTCATCCGCGTGGATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000709 unedited
  TGACCGCGGCCGCTTTTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTAAAAGCCAGCGCA
  GAGAGCAGTTTATTCGTGACCTCTGCTGGCCACTCGCACCTGCGCAAGTTCCACAGGCTG
  ACCTGCCATGTCCACCCCATGCCCAGAGGGGCTTCTAGTCCTGAATGTGCCCTCACCCAG
  CCCCTGGGAGAAGGCACTGTAACAACAGGTGAAGAGGGGGCTGACGGAGCCACAGCAACT
  GCTGCAGATGCTGTCCCCTGACAATGTAACAGCCGGGAGGAGCAGGGGTGAAGAGTGGCA
  GCCGCCCTGGCCGCTGAGTATTTTAGAGGGAGCTGACTGGGGAAGACAGTGGTGTGCTGT
  CAGGTCCCCCTGCTCCCCTTAGAGTGGGGCTACCTCTCGGGGTAGCTGAGGATGGGTGGG
  GGTGGGCTGAGCAGGTCTCACTTATCGAAGTGATCCAGTGGGTAGTGCTCCCCGTAGGTC
  TGCAGGTGGCGGGCCAGAGACTCCTGCTGCTTGCGGAGCTGGGCGGGCATCTCCTGATAC
  ACGTCTGAGAAGAGCAGGTTGGGGTTGGGTTTGGGCTTCCGCTCGGCCTGCTCAAAGGCC
  TCCATCACCTTCCTGCGGGACTGCTTCCTCCAGGCCTTCTCCTGCTCCTCATCCCACCAG
  NCCTTGGCTCACCAGATAGTGCCGCAGCCGGGAGATGGGGTGGCCCTGTTTATCCCATTA
  TTTGACCTCATCCACCGAGCGGTACGCTGAACTGCCGTCACTGGTGCTGCGGTGCCCGAT
  CCCTGTAGTCATGCCTCGAGACAAAGGTCTGGTTTCTGCCCAGCCCGCCGTGGGCCTCTT
  TGGGGGCTGTTACCGGAAAACCTCTTACCTCCCCCGGATTGACTGATGCCTACCCGGGGC
  TCGGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000709
• Insert Size: 1870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000709.2, NP_000700.1
• RefSeq Size: 1791 bp
• RefSeq ORF: 1791 bp
• Locus ID: 593
• UniProt ID: P12694
• Cytogenetics: 19q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation
• Gene Summary: The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).