Pancreatic alpha amylase (AMY2A) (NM_000699) Human Untagged Clone

CAT#: SC119712

AMY2A (untagged)-Human amylase, alpha 2A (pancreatic) (AMY2A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Pancreatic alpha amylase
• Synonyms: AMY2; PA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000699, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGTTCTTTCTGTTGCTTTTCACCATTGGGTTCTGCTGGGCTCAGTATTCCCCAAATACACAACAAG
  GACGGACATCTATTGTTCATCTGTTTGAATGGCGATGGGTTGATATTGCTCTTGAATGTGAGCGATATTT
  AGCTCCGAAGGGATTTGGAGGGGTTCAGGTCTCTCCACCAAATGAAAATGTTGCAATTTACAACCCTTTC
  AGACCTTGGTGGGAAAGATACCAACCAGTTAGCTATAAATTATGCACAAGATCTGGAAATGAAGATGAAT
  TTAGAAACATGGTGACTAGATGTAACAATGTTGGGGTTCGTATTTATGTGGATGCTGTAATTAATCATAT
  GTGTGGTAACGCTGTGAGTGCAGGAACAAGCAGTACCTGTGGAAGTTACTTCAACCCTGGAAGTAGGGAC
  TTTCCAGCAGTCCCATATTCTGGATGGGATTTCAATGATGGTAAATGTAAAACTGGAAGTGGAGATATCG
  AGAACTACAATGATGCTACTCAGGTCAGAGATTGTCGTCTGACTGGTCTTCTTGATCTTGCACTGGAGAA
  GGATTACGTGCGTTCTAAGATTGCCGAATATATGAACCATCTCATTGACATTGGTGTTGCAGGGTTCAGA
  CTTGATGCTTCCAAGCACATGTGGCCTGGAGACATAAAGGCAATTTTGGACAAACTGCATAATCTAAACA
  GTAACTGGTTCCCTGCAGGAAGTAAACCTTTCATTTACCAGGAGGTAATTGATCTGGGTGGTGAGCCAAT
  TAAAAGCAGTGACTACTTTGGTAATGGCCGGGTGACAGAATTCAAGTATGGTGCAAAACTCGGCACAGTT
  ATTCGCAAGTGGAATGGAGAGAAGATGTCTTACTTAAAGAACTGGGGAGAAGGTTGGGGTTTCGTACCTT
  CTGACAGAGCGCTTGTCTTTGTGGATAACCATGACAATCAACGAGGACATGGGGCTGGAGGAGCCTCTAT
  TCTTACCTTCTGGGATGCTAGGCTGTACAAAATGGCAGTTGGATTTATGCTTGCTCATCCTTACGGATTT
  ACACGAGTAATGTCAAGCTACCGTTGGCCAAGACAGTTTCAAAATGGAAACGATGTTAATGATTGGGTTG
  GGCCACCAAATAATAATGGAGTAATTAAAGAAGTTACTATTAATCCAGACACTACTTGTGGCAATGACTG
  GGTCTGTGAACATCGATGGCGCCAAATAAGGAACATGGTTATTTTCCGCAATGTAGTGGATGGCCAGCCT
  TTTACAAATTGGTATGATAATGGGAGCAACCAAGTGGCTTTTGGGAGAGGAAACAGAGGATTCATTGTTT
  TCAACAATGATGACTGGTCATTTTCTTTAACTTTGCAAACTGGTCTTCCTGCTGGCACATACTGTGATGT
  CATTTCTGGAGATAAAATTAATGGCAATTGCACAGGCATTAAAATTTACGTTTCTGATGATGGCAAAGCT
  CATTTTTCTATTAGTAACTCTGCTGAAGATCCATTTATTGCAATTCATGCTGAATCTAAATTGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000699 unedited
  NGTTGCAGCAATTNTGTATACGATCTCACTATAGGGCGGCCGCGAATCGGCACGAGGATG
  GAACTGAATATAAATAGTTTCTGGAAGGACACTGACAACTTCAAAGCAAAATGAAGTTCT
  TTCTGTTGCTTTTCACCATTGGGTTCTGCTGGGCTCAGTATTCCCCAAATACACAACAAG
  GACGGACATCTATTGTTCATCTGTTTGAATGGCGATGGGTTGATATTGCTCTTGAATGTG
  AGCGATATTTAGCTCCCAAGGGATTTGGAGGGGTTCAGGTCTCTCCACCAAATGAAAATG
  TTGCAATTCACAACCCTTTCAGACCTTGGTGGGAAAGATACCAACCAGTTAGCTATAAAT
  TATGCACAAGATCTGGAAATGAAGATGAATTTAGAAACATGGTGACTAGATGTAACAATG
  TTGGGGTTCGTATTTATGTGGATGCTGTAATTAATCATATGTCTGGTAATGCTGTGAGTG
  CAGGAACAAGCAGTACCTGTGGAAGTTACTTCAACCCTGGAAGTAGGGACTTTCCAGCAG
  TCCCATATTCTGGATGGGATTTTAATGATGGTAAATGTAAAACTGGAAGTGGAGATATCG
  AGAACTACAATGATGCTACTCAGGTCAGAGATTGTCGTCTGGTTGGTCTTCTTGATCTTG
  CACTGGAGAAAGATTATGTGCGTTCCAAGATTGCCGAATATATGAATCATCTCATTGACA
  TTGGTGTTGCAGGGTTCAGACTTGATGCTTCCAAGCACATGTGGCCTGNAGACATAAAGG
  CAATTTTGGNNACAACTGCATAATCTAAACAGTAACTGGTTCCCTGCCAGAAAGTAACCT
  TTCATTTACCAGNAGGTAATTGATCTGGGGTGGTGAGCCAATTAAAAGCAGTGACTACTT
  TGGAAATGGCCCGGTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000699 unedited
  GGAAACCTCCAGTTCCAGNAGNAGCACTGGGGCAGGGGTCACAGGGATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTTTGGTTTGAGGATATGCATTTAATTTTAAATTTTATAATTTAGATTCAGCATGAATT
  GCAATAAATGGATCCTCAGCAGAGTTACTAATTTAAAAATGAGCTTTGCCATCGTCAGAA
  ACGTAAATTTTAATGCCTGTGCAATTGCCATTAATTTTATCTCCAGAAATGACATCACAG
  TATGTGCCAGCAGGAAGACCAGTTTGCAAAGTTAAAGAAAATGTCCAGTCATCATTGTTG
  AAAACAATGAATCCTCTGTTTCCTCTCCCAAAAGCCACTTGGTTGCTCCCATTATCATAC
  CAGTTTGTAAAAGGCTGGCCATCCACTACATTGCGGAAATTAACCATGTTCCTTATTTGG
  CGCCATCGATGTTCACAGACCCAGTCATTGCCACAAGTAGTGTCTGGATTAATAGTAACT
  TCTTTAATTACTCCATTATTATTTGGTGGCCCAACCCAATCATTAACATCGTTTCCATTT
  TGAAACTGTCTTGGCCAACGGTAGCTTGACATTACTCGTGTAAAACCATAAGGATGAGCA
  AGCATAAATCCAACTGCCATTTTATACAGCCTAGCATCCCAGAAGGTAAGAATAGAGGCT
  CCTCCAGCCCCATGTCCTCGTTGATTGTCATGGNTATCCACAAAGACAAGTGCTCTGTCA
  GAAAGCATGAACCCCAACCTTCTTCCAGATCTTTAGTAAGACATCTTCTCTTCATTCACT
  TGCGAATACTGTGCCGAGTTTTGCACATACTTGAATCCTGTCACCCGGCATTTCCAAGTA
  TCACTGCTTTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000699
• Insert Size: 1660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000699.2, NP_000690.1
• RefSeq Size: 1660 bp
• RefSeq ORF: 1536 bp
• Locus ID: 279
• UniProt ID: P04746
• Cytogenetics: 1p21.1
• Domains: alpha-amylase, Aamy_C, Aamy
• Protein Families: Secreted Protein
• Protein Pathways: Metabolic pathways, Starch and sucrose metabolism
• Gene Summary: This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jan 2015]