CYP51A1 (NM_000786) Human Untagged Clone

CAT#: SC119686

CYP51A1 (untagged)-Human cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP51A1
• Synonyms: CP51; CYP51; CYPL1; LDM; P450-14DM; P450L1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119686 sequence for NM_000786 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGCGGCTGGGATGCTGCTGCTGGGCTTGCTGCAGGCGGGTGGGTCGGTGCTG
  GGCCAGGCGATGGAGAAGGTGACAGGCGGCAACCTCTTGTCCATGCTGCTGATCGCCTGC
  GCCTTCACCCTCAGCCTGGTCTACCTGATCCGTCTGGCCGCCGGCCACCTGGTCCAGCTG
  CCCGCAGGGGTGAAAAGTCCTCCATACATTTTCTCCCCAATTCCATTCCTTGGGCATGCC
  ATAGCATTTGGGAAAAGTCCAATTGAATTTCTAGAAAATGCATATGAGAAGTATGGACCT
  GTATTTAGTTTTACCATGGTAGGCAAGACATTTACTTACCTTCTGGGGAGTGATGCTGCT
  GCACTGCTTTTTAATAGTAAAAATGAAGACCTGAATGCAGAAGATGTCTACAGTCGCCTG
  ACAACACCTGTGTTTGGGAAGGGAGTTGCATACGATGTGCCTAATCCAGTTTTCTTGGAG
  CAGAAGAAAATGTTAAAAAGTGGCCTTAACATAGCCCACTTTAAACAGCATGTTTCTATA
  ATTGAAAAAGAAACAAAGGAATACTTTGAGAGTTGGGGAGAAAGTGGAGAAAAAAATGTG
  TTTGAAGCTCTTTCTGAGCTCATAATTTTAACAGCTAGCCATTGTTTGCATGGAAAGGAA
  ATCAGAAGTCAACTCAATGAAAAGGTAGCACAGCTGTATGCAGATTTGGATGGAGGTTTC
  AGCCATGCAGCCTGGCTCTTACCAGGTTGGCTGCCTTTGCCTAGTTTCAGACGCAGGGAC
  AGAGCTCATCGGGAAATCAAGGATATTTTCTATAAGGCAATCCAGAAACGCAGACAGTCT
  CAAGAAAAAATTGATGACATTCTCCAAACTTTACTAGATGCTACATACAAGGATGGGCGT
  CCTTTGACTGATGATGAAGTAGCAGGGATGCTTATTGGATTACTCTTGGCAGGGCAGCAT
  ACATCCTCAACTACTAGTGCTTGGATGGGCTTCTTTTTGGCCAGAGACAAAACACTTCAA
  AAAAAATGTTATTTAGAACAGAAAACAGTCTGTGGAGAGAATCTGCCTCCTTTAACTTAT
  GACCAGCTCAAGGATCTAAATTTACTTGATCGCTGTATAAAAGAAACATTAAGACTTAGA
  CCTCCTATAATGATCATGATGAGAATGGCCAGAACTCCTCAGACTGTGGCAGGGTATACC
  ATTCCTCCAGGACATCAGGTGTGTGTTTCTCCCACTGTCAATCAAAGACTTAAAGACTCA
  TGGGTAGAACGCCTGGACTTTAATCCTGATCGCTACTTACAGGATAACCCAGCATCAGGG
  GAAAAGTTTGCCTATGTGCCATTTGGAGCTGGGCGTCACCGTTGTATTGGGGAAAATTTT
  GCCTATGTTCAAATTAAGACAATTTGGTCCACTATGCTTCGTTTATATGAATTTGATCTC
  ATTGATGGATACTTTCCCACTGTGAATTATACAACTATGATTCACACCCCTGAAAACCCA
  GTTATCCGTTACAAACGAAGATCAAAATGA
  
  Clone variation with respect to NM_000786.3
  1359 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000786 unedited
  GGATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGCTGCCAGCTT
  CTCTCGTTCCGTCGATTGGGAGGAGCGGTGGCGACCTCGGCCTTCAGTGTTTCCGACGGA
  GTGAATGGCGGCGGCGGCTGGGATGCTGCTGCTGGGCTTGCTGCAGGCGGGTGGGTCGGT
  GCTGGGCCAGGCGATGGAGAAGGTGACAGGCGGCAACCTCTTGTCCATGCTGCTGATCGC
  CTGCGCCTTCACCCTCAGCCTGGTCTACCTGATCCGTCTGGCCGCCGGCCACCTGGTCCA
  GCTGCCCGCAGGGGTGAAAAGTCCTCCATACATTTTCTCCCCAATTCCATTCCTTGGGCA
  TGCCATAGCATTTGGGAAAAGTCCAATTGAATTTCTAGAAAATGCATATGAGAAGTATGG
  ACCTGTATTTAGTTTTACCATGGTAGGCAAGACATTTACTTACCTTCTGGGGAGTGATGC
  TGCTGCACTGCTTTTTAATAGTAAAAATGAAGACCTGAATGCAGAAGATGTCTACAGTCG
  CCTGACAACACCTGTGTTTGGGAAAGGGAGTTGCATACGATGTGCCTAATCCAGTTTTCT
  TGGAGCAGAAGAAATGTTAAAAAGTGGCCTTACATAGCCCACTTTAACAGCATGTTTCTA
  TTATTGAAAAAGAACAAGGAATCTTTGAGAGTTGGGGAAGAAAGTGAGAAAAAATGGGGT
  TGAAGCTCTTCTGACTCATATTTTACAGCTAGCCATTGTTGCATGGAAAGGAATTCAAAG
  TCACTCATGGAAAGGTACACAGCGATGCAATTGNNNGGGGGGGGGGTTTAACCTTGCACC
  TGGCTTTACCCGGTTGCTGGCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000786 unedited
  GTACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTCAAAAAAAATTTTATTCTGAAAATTACCAAAAATTTTGCCTTCCCA
  TTTAAAGTTTAACATGAGTCTTCCAGGGTTTAAAAAAAACCTGTATTTTCCTTAAGGCAA
  ATTTTTTGGGCTTCCCCTAGGGAAACCAACCGATGGGGTTTTTTTTCACCCTTCTCATTT
  AGTTGGGCCCCGAGTTCCAATTGGAAATAAGTGGATTCCTAAACAAAAGGGGCTTTACAA
  AAGGGGCCCATTTTTTTTCCTCCCAGAGGCCCTTGTTTCAAATTTTTTTAAACCCAAAGT
  TCACTGATCTCACTTTTAAAAAAAAAAAAAGTTGGTTACCGGTTTTAATCAATTTGAGTG
  GTACCAACCTTCCCTAATGGGATTCTAAAAAGGAACCAGGGAAGGCCAGGGGCGGGGGCT
  CATGCCTGTAATCCCACCCCTTTGGGAGGCCAAGGGGGGGGGATCACAAGGTCAGGATTT
  CAAAACCACCCTACCCAACTTAGGGAACCCCCTGTCTTTCCTAAAAATCCAAAAATTTAC
  CCAGCCTTGGGGGCAGGGCCCTGTAATCCCAACTCCTTGGGAGGCTGAGGCAAAAAATTG
  CTTGACCCCGGGAGGCAAAGGATGCATTGACCTGAAATCGCCCCACTGCCTCTCAGCCTG
  GGCAACAGAGCGAGACTCCTTCTCAAAAAAAAAAAAAAAAGGGAAGCGGGGAACACCTGA
  GCCAAAGCAAATTATCCTCTAGGACTTGACTTTGGACTCCACATAAGGGGACCTTGGAAA
  ACTAAATCCTTTTTTCAGGAAAAAAATTGGTATTTTTTANAATTTGACCAAGAACTGGAC
  CGCCTTTACATAATAATTACATTTTTTAGGATTCTATCAATTGGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000786
• Insert Size: 3090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000786.2, NP_000777.1
• RefSeq Size: 3381 bp
• RefSeq ORF: 1530 bp
• Locus ID: 1595
• UniProt ID: Q16850
• Cytogenetics: 7q21.2
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).