RPL7 (NM_000971) Human Untagged Clone

CAT#: SC119573

RPL7 (untagged)-Human ribosomal protein L7 (RPL7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL7
• Synonyms: humL7-1; L7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000971, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGGTGTAGAAGAGAAGAAGAAGGAGGTTCCTGCTGTGCCAGAAACCCTTAAGAAAAAGCGAAGGA
  ATTTCGCAGAGCTGAAGATCAAGCGCCTGAGAAAGAAGTTTGCCCAAAAGATGCTTCGAAAGGCAAGGAG
  GAAGCTTATCTATGAAAAAGCAAAGCACTATCACAAGGAATATAGGCAGATGTACAGAACTGAAATTCGA
  ATGGCGAGGATGGCAAGAAAAGCTGGCAACTTCTATGTACCTGCAGAACCCAAATTGGCGTTTGTCATCA
  GAATCAGAGGTATCAATGGAGTGAGCCCAAAGGTTCGAAAGGTGTTGCAGCTTCTTCGCCTTCGTCAAAT
  CTTCAATGGAACCTTTGTGAAGCTCAACAAGGCTTCGATTAACATGCTGAGGATTGTAGAGCCATATATT
  GCATGGGGGTACCCCAATCTGAAGTCAGTAAATGAACTAATCTACAAGCGTGGTTATGGCAAAATCAATA
  AGAAGCGAATTGCTTTGACAGATAACGCTTTGATTGCTCGATCTCTTGGTAAATACGGCATCATCTGCAT
  GGAGGATTTGATTCATGAGATCTATACTGTTGGAAAACGCTTCAAAGAGGCAAATAACTTCCTGTGGCCC
  TTCAAATTGTCTTCTCCACGAGGTGGAATGAAGAAAAAGACCACCCATTTTGTAGAAGGTGGAGATGCTG
  GCAACAGGGAGGACCAGATCAACAGGCTTATTAGAAGAATGAACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000971 unedited
  CAGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGNAACCAT
  GGAGGGTGTAGAAGAGAAGAAGAAGGAGGTTCCTGCTGTGCCAGAAACCCTTAAGAAAAA
  GCGAAGGAATTTCGCAGAGCTGAAGATCAAGCGCCTGAGAAAGAAGTTTGCCCAAAAGAT
  GCTTCGAAAGGCAAGGAGGAAGCTTATCTATGAAAAAGCAAAGCACTATCACAAGGAATA
  TAGGCAGATGTACAGAACTGAAATTCGAATGGCGAGGATGGCAAGAAAAGCTGGCAACTT
  CTATGTACCTGCAGAACCCAAATTGGCGTTTGTCATCAGAATCAGAGGTATCAATGGAGT
  GAGCCCAAAGGTTCGAAAGGTGTTGCAGCTTCTTCGCCTTCGTCAAATCTTCAATGGAAC
  CTTTGTGAAGCTCAACAAGGCTTCGATTAACATGCTGAGGATTGTAGAGCCATATATTGC
  ATGGGGGTACCCCAATCTGAAGTCAGTAAATGAACTAATCTACAAGCGTGGTTATGGCAA
  AATCAATAAGAAGCGAATTGCTTTGACAGATAACGCTTTGATTGCTCGATCTCTTGGTAA
  ATACGGCATCATCTGCATGGAGGATTTGATTCATGAGATCTATACTGTTGGAAAACGCTT
  CAAAGAGGCAAATAACTTCCTGTGGCCCTTCAAATTGTCTTCTCCACGAGGTGGAATGAA
  GAAAAAGACCACCCATTTTGTANAAGGTGGAGATGCTGGGCACAGGGAGGACCAGATCAA
  CAGGCTTATTAAAAGAATAAACTAAGGTGTCTACCATGATTATTTTTCTAAGCTGGTTGG
  TTAATAAACAGTACCTGCTCTCAAATTGAAAAAAAAAAAAAAACTCGACTCTANTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000971 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAATTTGAGAGCAGGTACTGTTT
  ATTAACCAACCAGCTTAGAAAAATAATCATGGTAGACACCTTAGTTTATTCTTTTAATAA
  GCCTGTTGATCTGGTCCTCCCTGTTGCCAGCATCTCCACCTTCTACAAAATGGGTGGTCT
  TTTTCTTCATTCCACCTCGTGGAGAAGACAATTTGAAGGGCCACAGGAAGTTATTTGCCT
  CTTTGAAGCGTTTTCCAACAGTATAGATCTCATGAATCAAATCCTCCATGCAGATGATGC
  CGTATTTACCAAGAGATCGAGCAATCAAAGCGTTATCTGTCAAAGCAATTCGCTTCTTAT
  TGATTTTGCCATAACCACGCTTGTAGATTAGTTCATTTACTGACTTCAGATTGGGGTACC
  CCCATGCAATATATGGCTCTACAATCCTCAGCATGTTAATCGAAGCCTTGTTGAGCTTCA
  CAAAGGTTCCATTGAAGATTTGACGAAGGCGAAGAAGCTGCAACACCTTTCGAACCTTTG
  GGCTCACTCCATTGATACCTCTGATTCTGATGACAAACGCCAATTTGGGTTCTGCAGGTA
  CATAGAAGTTGCCAGCTTTTCTTGCCTCCTCGCCATTCAATTTCAGTTCTGACATCCGCC
  TATATTCCTTGAGATAGAGCTTTGCTCTTTCATAGATAAGCTTCCTCCTTGCCTTTCGAA
  GCATTTTTTTGAGCAACCTCCTTTCTCAGGCGCTTGGATCTTAGCTCTGCGAAATTCTTC
  GCTTTTTTTTAAGCTTTCTGGCCAACAGGAACTTCTTCTTCTTTTTTCTACCCCTCCATG
  GGTCCTCGGGCGGATTCCGGCCCCCCATAGGGAGCCGATTAAAATTCTGACGGTTACCTA
  AGAACCTGCTTAAAAACCCCCACCGCCACCCTCCCCCATTGCGTAACGGGCGGGGTTTCC
  ACCTTTGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000971
• Insert Size: 750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000971.3, NP_000962.2
• RefSeq Size: 866 bp
• RefSeq ORF: 747 bp
• Locus ID: 6129
• UniProt ID: P18124
• Cytogenetics: 8q21.11
• Domains: Ribosomal_L30
• Protein Families: Transcription Factors
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]