RPB2 (POLR2B) (NM_000938) Human Untagged Clone

CAT#: SC119548

POLR2B (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPB2
• Synonyms: hRPB140; POL2RB; RPB2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000938, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTACGACGCGGATGAGGATATGCAATATGATGAGGATGATGATGAAATCACCCCGGATTTGTGGCAAG
  AAGCATGCTGGATTGTAATCAGTTCCTATTTTGACGAGAAAGGCTTGGTTAGACAACAGCTGGATTCTTT
  TGATGAGTTTATTCAGATGTCTGTTCAAAGAATTGTGGAAGACGCTCCTCCTATAGACCTACAGGCTGAA
  GCTCAGCATGCTAGTGGAGAAGTTGAAGAACCGCCACGATATTTGCTGAAGTTTGAACAAATTTATCTTT
  CCAAGCCTACCCATTGGGAAAGAGATGGTGCTCCTTCACCAATGATGCCCAATGAAGCTAGATTAAGGAA
  TCTCACGTATTCTGCTCCGCTTTATGTTGATATAACAAAAACAGTCATTAAAGAAGGTGAAGAACAACTT
  CAGACTCAGCATCAGAAAACTTTTATAGGAAAAATTCCAATTATGTTGCGGTCAACTTACTGCCTTTTGA
  ATGGCTTGACAGATCGTGATCTTTGTGAGTTAAATGAATGCCCTTTGGATCCTGGTGGCTATTTCATTAT
  TAATGGATCAGAAAAGGTTCTGATTGCCCAAGAGAAAATGGCAACAAACACAGTTTATGTGTTTGCCAAA
  AAGGATTCTAAATATGCCTACACAGGAGAGTGTAGATCATGTCTTGAGAATTCTTCCCGACCCACCAGTA
  CTATATGGGTTAGCATGCTGGCAAGAGGAGGACAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGT
  GGCAACTCTACCATATATCAAGCAAGAAGTTCCCATCATTATTGTGTTCAGAGCATTAGGTTTTGTGTCC
  GACAGAGATATTTTAGAACATATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGTTAAACCTT
  CTCTCGATGAAGCTTTTGTCATCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCACGAGGAGCAAA
  GCCTGGTGTTACTAAAGAGAAAAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCAT
  GTTGGTGTCAGTGATTTTTGTGAGACCAAAAAAGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTC
  TGGCAGCTTTGGGTAGAAGAGAACTAGATGACAGAGATCACTATGGAAACAAGAGATTGGATCTTGCTGG
  GCCGCTGCTTGCATTCTTATTTAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAG
  AAATTTATTGATCGAGGAAAGGATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGCC
  TAAAATACTCTTTAGCTACTGGAAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTATC
  TCAGGTGTTAAACCGCCTGACTTTTGCGTCTACTCTTTCTCACCTGCGTCGTTTAAATTCTCCTATTGGT
  AGAGACGGCAAGCTAGCAAAACCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCCTGCCGAGA
  CCCCAGAGGGCCATGCTGTAGGACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGATCTCA
  ACCATCTCCAATTCTGGAATTTTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCTGCAGCT
  ATTGCTGATGCAACCAAGATTTTTGTTAATGGCTGCTGGGTTGGAATACATAAAGATCCCGAACAACTTA
  TGAACACCCTAAGGAAATTGAGACGTCAGATGGACATCATTGTGTCTGAAGTTTCTATGATCAGAGATAT
  TCGAGAGAGGGAGATTCGGATCTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATTGTGGAAAAA
  CAAAAGCTACTTTTGAAGAAGAGGCATATTGACCAATTGAAAGAGAGAGAATATAACAACTATAGTTGGC
  AGGATCTTGTGGCCAGTGGGGTAGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTGATGCTTGCAAT
  GACTCCAGATGATTTACAGGAGAAAGAAGTAGCTTATTGTTCCACATATACACACTGTGAGATTCATCCC
  TCAATGATCCTTGGTGTCTGTGCATCTATTATTCCCTTTCCTGATCATAACCAGTCCCCTAGAAACACAT
  ACCAGTCTGCTATGGGTAAGCAGGCTATGGGAGTTTACATCACCAACTTCCATGTTCGCATGGACACATT
  GGCCCATGTTCTCTATTATCCTCAAAAGCCACTTGTGACTACACGGTCTATGGAATATCTACGATTTAGA
  GAGCTGCCAGCAGGCATCAACTCAATTGTGGCCATTGCATCATACACTGGATATAATCAGGAAGACTCTG
  TTATCATGAATCGTTCAGCTGTAGACCGCGGCTTCTTCAGGTCTGTTTTCTATCGCTCATACAAAGAACA
  GGAGTCTAAAAAAGGATTTGATCAAGAAGAAGTTTTTGAGAAGCCTACACGTGAAACATGCCAGGGCATG
  AGGCATGCCATTTACGACAAGCTGGATGATGATGGTTTGATAGCTCCAGGGGTTCGTGTATCAGGAGATG
  ATGTTATTATAGGCAAAACAGTCACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGACGCTA
  TACCAAGAGAGACTGTAGCACTTTTCTCAGAACTAGTGAGACGGGCATTGTGGATCAGGTTATGGTAACT
  CTCAATCAGGAAGGATATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGGATTCCACAGATTGGAGACA
  AATTTGCTAGTCGACATGGTCAAAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGATATGCCTTTCAC
  CTGTGAAGGTATCACCCCTGATATCATCATCAATCCCCATGCCATCCCCTCTCGTATGACTATTGGTCAC
  TTAATTGAATGCCTTCAAGGGAAGGTATCGGCTAACAAGGGTGAAATTGGTGATGCCACTCCATTTAATG
  ATGCTGTTAACGTGCAGAAGATTTCTAATCTTTTATCTGATTATGGCTATCATCTCAGAGGAAATGAGGT
  CCTGTACAATGGGTTCACTGGTCGAAAAATCACATCACAAATATTTATTGGCCCCACTTATTACCAGCGT
  TTGAAGCATATGGTGGATGATAAGATTCACTCTCGTGCTAGGGGACCTATTCAGATCCTCAATAGACAGC
  CCATGGAGGGTAGATCTCGTGATGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTGCCCA
  TGGAGCAGCCCAGTTTTTAAGGGAAAGATTGTTTGAGGCATCAGATCCATATCAGGTTCATGTTTGCAAT
  CTTTGTGGAATAATGGCGATTGCCAACACCAGGACCCATACATATGAATGCAGGGGCTGCCGCAATAAAA
  CCCAGATTTCTTTGGTGCGAATGCCTTACGCATGCAAACTATTGTTTCAGGAACTTATGTCTATGAGTAT
  TGCACCGCGAATGATGAGTGTTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000938 unedited
  TTATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTGCTGGCTTCTC
  GGGCGGTTTTTGTCTTTTGATTTCAAGAGTTAGGAGCTCGAGAACCGTTTGGCAATATGT
  ACGACGCGGATGAGGATATGCAATATGATGAGGATGATGATGAAATCACCCCGGATTTGT
  GGCAAGAAGCATGCTGGATTGTAATCAGTTCCTATTTTGACGAGAAAGGCTTGGTTAGAC
  AACAGCTGGATTCTTTTGATGAGTTTATTCAGATGTCTGTTCAAAGAATTGTGGAAGACG
  CTCCTCCTATAGACCTACCTGCTGAAGCTCAGCATGCTAGTGGAGAAGTTGAAGAACCGC
  CACGATATTTGCTGAAGTTTGAACAAATTTATCTTTCCAAGCCTACCCATTGGGAAAGAG
  ATGGTGCTCCTTCACCAATGATGCCCAATGAAGCTAGATTAAGGAATCTCACGTATTCTG
  CTCCGCTCTATGTTGATATAACAAAAACAGTCATTAAAGAAGGTGAAGAACAACTTCAGA
  CTCATCATCACAAAACTTTTATAGGAAAAATTCCAATTATGCTGCGGTCAACTTACTGCC
  TTTTGAATGGCTTGACAGAACGTGATCTTTGTGAGTTAAATGAATGCCCTTTGGATCCTG
  CGGGCTATTTCATTATTAATGGATCAGAAAAGGCTCTGATTGCCCAAGAGAAAATGGCAC
  CAAACACAGTTAATGTGTTCGCCAAAAGGATTCTAAATATGCCTACACAGGAGAGTGCAC
  ATCATGTCTTGAGAATTCCTCCGACCCACCAGTACTATATGGGCTACCATGCTGGCACCA
  GGAGACAGGGTGCCCANAATAGTGCTATTGGTCACCGCATTGTGGCACCTCTACCATATT
  AAACCAAGAAGTCTCATCATTATGTGGTCAAACCACTAGTTTTCGCGCCCGACCAAAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000938 unedited
  ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCTATCTACAAAAATT
  TATTATATATTTACAGAAGAAAAGCATGCATATCATTAAACAAATAAAATACTTTTTATC
  ACAACACAGTACATATTTGTCATTTTTAAAAAGCCACACAATAGAAACAAGACACCAAGA
  TATTTAATTATCTTGTTGACTCCTGTAAAATAGCTAAACACTCATCATTCGCGGTGCAAT
  ACTCATAGACATAAGTTCCTGAAACAATAGTTTGCATGCGTAAGGCATTCGCACCAAAGA
  AATCTGGGTTTTATTGCGGCAGCCCCTGCATTCATATGTATGGGTCCTGGTGTTGGCAAT
  CGCCATTATTCCACAAAGATTGCAAACATGAACCTGATATGGATCTGATGCCTCAAACAA
  TCTTTCCCTTAAAAACTGGGCTGCTCCATGGGCAATCTGACAATCTCGTTCCATTTCTCC
  AAAACGCAGGCCACCATCACGAGATCTACCCTCCATGGGCTGTCTATTGAGGATCTGAAT
  AGGTCCCCTAGCACGAGAGTGAATCTTATCATCCACCATATGCTTCAAACGCTGGTAATA
  AGTGGGGCCAATAAATATTTGTGATGTGGATTTTCGACCAGTGAACCCATTGTACAGGAC
  CTCATTTTCTCTGAGATGATTGCCCATATCAGATTAAAGATTAGAAATCTTCTGCACGTT
  AACAGCATCATAAAATGGAGTGGGATCACCATTTCACCCCTGGTTAGCCATACCTTCCCT
  TGAAAGCATTCATTAAAGGGACCATAGTCCTACCAAAGGGGGATGGCTGGGGGATTGATG
  ATGATATCAAGGGTGGAAACCTTCCACGTGAAAAGGCTATCCCTTTGTCTTTACTGAAAC
  CACAAGTACCCTTTTGACATGTGGACTAGCAAATTGGGTCACAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000938
• Insert Size: 4150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000938.1, NP_000929.1
• RefSeq Size: 3748 bp
• RefSeq ORF: 3525 bp
• Locus ID: 5431
• UniProt ID: P30876
• Cytogenetics: 4q12
• Domains: RNA_pol_Rpb2_6, RNA_pol_Rpb2_7, RNA_pol_Rpb2_2, RNA_pol_Rpb2_1, RNA_pol_Rpb2_3, RNA_pol_Rpb2_4, RNA_pol_Rpb2_5
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (1) encodes the longest isoform (1).