Ribosomal protein S10 (RPS10) (NM_001014) Human Untagged Clone

CAT#: SC119522

RPS10 (untagged)-Human ribosomal protein S10 (RPS10), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Ribosomal protein S10
• Synonyms: DBA9; S10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119522 sequence for NM_001014 edited (data generated by NextGen Sequencing)
  ATGTTGATGCCTAAGAAGAACCGGATTGCCATTTATGAACTCCTTTTTAAGGAGGGAGTC
  ATGGTGGCCAAGAAGGATGTCCACATGCCTAAGCACCCGGAGCTGGCAGACAAGAATGTG
  CCCAACCTTCATGTCATGAAGGCCATGCAGTCTCTCAAGTCCCGAGGCTACGTGAAGGAA
  CAGTTTGCCTGGAGACATTTCTACTGGTACCTTACCAATGAGGGTATCCAGTATCTCCGT
  GATTACCTTCATCTGCCCCCGGAGATTGTGCCTGCCACCCTACGCCGTAGCCGTCCAGAG
  ACTGGCAGGCCTCGGCCTAAAGGTCTGGAGGGTGAGCGACCTGCGAGACTCACAAGAGGG
  GAAGCTGACAGAGATACCTACAGACGGAGTGCTGTGCCACCTGGTGCCGACAAGAAAGCC
  GAGGCTGGGGCTGGGTCAGCAACCGAATTCCAGTTTAGAGGCGGATTTGGTCGTGGACGT
  GGTCAGCCACCTCAGTAA
  
  Clone variation with respect to NM_001014.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001014 unedited
  CACGAGGCCTGCAGCCGCAGAGATGTTGATGCCTAAGAAGAACCGGATTGCCATTTATGA
  ACTCCTTTTTAAGGAGGGAGTCATGGTGGCCAAGAAGGATGTCCACATGCCTAAGCACCC
  GGAGCTGGCAGACAAGAATGTGCCCAACCTTCATGTCATGAAGGCCATGCAGTCTCTCAA
  GTCCCGAGGCTACGTGAAGGAACAGTTTGCCTGGAGACATTTCTACTGGTACCTTACCAA
  TGAGGGTATCCAGTATCTCCGTGATTACCTTCATCTGCCCCCGGAGATTGTGCCTGCCAC
  CCTACGCCGTAGCCGTCCAGAGACTGGCAGGCCTCGGCCTAAAGGTCTGGAGGGTGAGCG
  ACCTGCGAGACTCACAAGAGGGGAAGCTGACAGAGATACCTACAGACGGAGTGCTGTGCC
  ACCTGGTGCCGACAAGAAAGCCGAGGCTGGGGCTGGGTCAGCAACCGAATTCCAGTTTAG
  AGGCGGATTTGGTCGTGGACGTGGTCAGCCACCTCAGTAAAATTGGAGAGGATTCTTTTG
  CATTGAATAAACTTACAGCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001014 unedited
  TGAAGTTTCATCATGGCAAAATATCCTCTCCATTTTACTGAGGTGGCTGACCACGTCCAC
  GACCAAATCCGCCTCTAAACTGGAATTCGGTTGCTGACCCAGCCCCAGCCTCGGCTTTCT
  TGTCGGCACCAGGTGGCACAGCACTCCGTCTGTAGGTATCTCTGTCAGCTTCCCCTCTTG
  TGAGTCTCGCAGGTCGCTCACCCTCCATACCTTTATGCCGAGGCCTGCCAGTCTCTGGAC
  GGCTACGGCGTAAGGTGGCAGGCACAATCTCCGGGGGCAGATGAAGGTAATCACGGAGAT
  ACTGGATACCCTCATTGGTAAGGTACCAGTAGAAATGTCTCCAGGCAAACTGTTCCTTCA
  CGTAGCCTCGGGACTTGAGAGACTGCATGGCCTTCATGACATGAAGGTTGGGCACATTCT
  TGTCTGCCAGCTCCGGGTGCTTATGCATGTGGACATCCTTCTTGGCCACCATGACTCCCT
  CCTTAAAAAGGAGTTCATAAATGGCAATCCGGTTCTTCTTATGCATCAACATCTCTGCGG
  CTGCAGGCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGA
  CGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATT
  TGCGTCAACGGGGCGGAGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAA
  AACATACTCCCATTGACGTCAATGGGGGTGGAGACTTGGAAATCCCCGTGAGTCATACCG
  CTATCCACGCCCATTGGGTGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTA
  ATACGTAGATGTACTGCCCAGTAGGAAAGTCCNGTAAGGTCATGTACTGGGCATAAATGC
  CAGCGGGCCATTTACCGTCATTGACGTCATTAGGGGCGGGACTGGCATATGATCACTCTG
  AGTACTGCCCAGTGGGCAGNTTACCGTAATACTCACCCATGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001014
• Insert Size: 670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014.3, NP_001005.1
• RefSeq Size: 636 bp
• RefSeq ORF: 498 bp
• Locus ID: 6204
• UniProt ID: P46783
• Cytogenetics: 6p21.31
• Domains: S10_plectin
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternate splicing results in multiple transcript variants that encode the same protein. Naturally occurring read-through transcription occurs between this locus and the neighboring locus NUDT3 (nudix (nucleoside diphosphate linked moiety X)-type motif 3).[provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.