RPL34 (NM_000995) Human Untagged Clone

CAT#: SC119502

RPL34 (untagged)-Human ribosomal protein L34 (RPL34), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL34
• Synonyms: L34
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119502 sequence for NM_000995 edited (data generated by NextGen Sequencing)
  ATGGTCCAGCGTTTGACATACCGACGTAGGCTTTCCTACAATACAGCCTCTAACAAAACT
  AGGCTGTCCCGAACCCCTGGTAATAGAATTGTTTACCTTTATACCAAGAAGGTTGGGAAA
  GCACCAAAATCTGCATGTGGTGTGTGCCCAGGCAGACTTCGAGGGGTTCGTGCTGTAAGA
  CCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCAGCAGGGCCTATGGTGGT
  TCCATGTGTGCTAAATGTGTTCGTGACAGGATCAAGCGTGCTTTCCTTATCGAGGAGCAG
  AAAATCGTTGTGAAAGTGTTGAAGGCACAAGCACAGAGTCAGAAAGCTAAATAA
  
  Clone variation with respect to NM_000995.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000995 unedited
  TGCACTATTTTGTNATCACGACTCACTATAGGGCGGCCGCGCATTCGGCACGAGGCTGCA
  GGCACTCAGAATGGTCCAGCGTTTGACATACCGACGTAGGCTTTCCTACAATACAGCCTC
  TAACAAAACTAGGCTGTCCCGAACCCCTGGTAATAGAATTGTTTACCTTTATACCAAGAA
  GGTTGGGAAAGCACCAAAATCTGCATGTGGTGTGTGCCCAGGCAGACTTCGAGGGGTTCG
  TGCTGTAAGACCTAAAGTTCTTATGAGATTGTCCAAAACAAAGAAACATGTCAGCAGGGC
  CTATGGTGGTTCCATGTGTGCTAAATGTGTTCGTGACAGGATCAAGCGTGCTTTCCTTAT
  CGAGGAGCAGAAAATCGTTGTGAAAGTGTTGAAGGCACAAGCACAGAGTCAGAAAGCTAA
  ATAAAAAAATGAAACTTTTTTGAGTAATAAAAAATGAAAAGACGCTGTCCAATAGAAAAA
  GTTGGTGTGCTGGAGCTACCTCACCTCAGCTTGAGAGAGCCAGTTGTGTGCATCTCTTTC
  CAGCTTTGCATTCAGTGACGTCTGCTTGGCATTTTGAGATTGTTATGGTGAGAGTATTTT
  ACACCCTCAGCATATGCCTGCAAAATCCTGTTTTCCCCCCAGAGAGCTGGAGGTTTAATA
  CTACCAGCACATTCCCTAGAATACCTACTCAAGTTACAGTATATGATCACTAATATAGCA
  TGCTTCTTGGTACCACGCAGCTCTTGATCTATAATCTGGGACCTGTCTGGATAATGACTT
  TGACTGTTATAATACAGCCTTATTAATACTTTCAATCCTGTCAAGAGTTTAAGGCCTGAT
  TTTTATAAACTCCAAAAGAACCTTTTTTTTGCAAAAAAAAAAAAAAACTCCACTTTACAA
  TGCGGCCGCGGCCATAACTTGTTCCTGAACCAAATCCGGGCTGCACTCCTTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000995 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAATAAAAGGCTCATTT
  TGGATTTTATAAAATCAAACCCTAAACTCTTTACAGAATCGAAGTATTAATAAGTACTTA
  TAATAACAATCAAGTCATTATCAAACATGTACCAGATATATATCAGAGCTCCTGGTACCA
  AGAGCATACTATATTAGTGATCATATACTGTAACTTGAGTAGTATCTAGGGATGTGCTGG
  TAGTATTTAACCTCCAGCTCTCTGGGGGAAAACAGGATTTTGCAGCATTTGCTGAGGTGT
  AAATACTCTCACCATAACAATCTCAAGATGCCAAGCAGACGTCACTGGATGCAAAACTGG
  AAAGAGATGCACACAACTGGCTCTCTCAAGCTGAGGTGAGGTAGCTCCAGCACACCAACT
  TTTTCTATTGGACAGCGTCTTTTCATTTTTTATTACTCAAAAAAGTTTCATTTTTTTATT
  TAGCTTTCTGACTCTGTGCTTGTGCCTTCAACACTTTCACAACGATTTTCTGCTCCTCGA
  TAAGGAAAGCACGCTTGATCCTGTCACGAACACATTTAGCACACATGGAACCACCATAGG
  CCCTGCTGACATGTTTCTTTGTTTTGGACAATCTCATAAGAACTTTAGGTCTTACAGCAC
  GAACCCCTCGAAGTCTGCCTGGGCACACACCACATGCAGATTTTGGTGCTTTCCCAACCT
  TCTTGGTATAAAGGTAAACAATNCTATTACCAGGGGGTCGGGGACAGCCTANTTTTGTTA
  GAGGCTGNATTGANGGAAACCTACCTCGGTATGTCAAACGCTGAACATTCTGAGTGCCTG
  CAGCCTCGTGCAGATTCGCGCCGCCCTTAAGGAGTGGTATACAAAATTTGACGGTTACCT
  AACGAGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000995
• Insert Size: 880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000995.2, NP_000986.2
• RefSeq Size: 849 bp
• RefSeq ORF: 354 bp
• Locus ID: 6164
• UniProt ID: P49207
• Cytogenetics: 4q25
• Domains: Ribosomal_L34e
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Overexpression of this gene has been observed in some cancer cells. Alternative splicing results in multiple transcript variants, all encoding the same isoform. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1-6 all encode the same protein.