RPL28 (NM_000991) Human Untagged Clone

CAT#: SC119498

RPL28 (untagged)-Human ribosomal protein L28 (RPL28), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL28
• Synonyms: L28
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119498 sequence for NM_000991 edited (data generated by NextGen Sequencing)
  ATGTCTGCGCATCTGCAATGGATGGTCGTGCGGAACTGCTCCAGTTTCCTGATCAAGAGG
  AATAAGCAGACCTACAGCACTGAGCCCAATAACTTGAAGGCCCGCAATTCCTTCCGCTAC
  AACGGACTGATTCACCGCAAGACTGTGGGCGTGGAGCCGGCAGCCGACGGCAAAGGTGTC
  GTGGTGGTCATTAAGCGGAGATCCGGCCAGCGGAAGCCTGCCACCTCCTATGTGCGGACC
  ACCATCAACAAGAATGCTCGCGCCACGCTCAGCAGCATCAGACACATGATCCGCAAGAAC
  AAGTACCGCCCCGACCTGCGCATGGCAGCCATCCGCAGGGCCAGCGCCATCCTGCGCAGC
  CAGAAGCCTGTGATGGTGAAGAGGAAGCGGACCCGCCCCACCAAGAGCTCCTGA
  
  Clone variation with respect to NM_000991.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000991 unedited
  TTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGCCGCTGCGGAG
  GGAGCCGCCGCCATGTCTGCGCATCTGCAATGGATGGTCGTGCGGAACTGCTCCAGTTTC
  CTGATCAAGAGGAATAAGCAGACCTACAGCACTGAGCCCAATAACTTGAAGGCCCGCAAT
  TCCTTCCGCTACAACGGACTGATTCACCGCAAGACTGTGGGCGTGGAGCCGGCAGCCGAC
  GGCAAAGGTGTCGTGGTGGTCATTAAGCGGAGATCCGGCCAGCGGAAGCCTGCCACCTCC
  TATGTGCGGACCACCATCAACAAGAATGCTCGCGCCACGCTCAGCAGCATCAGACACATG
  ATCCGCAAGAACAAGTACCGCCCCGACCTGCGCATGGCAGCCATCCGCAGGGCCAGCGCC
  ATCCTGCGCAGCCAGAAGCCTGTGATGGTGAAGAGGAAGCGGACCCGCCCCACCAAGAGC
  TCCTGAGCCCCCTGCCCCCAGAGCAATAAAGTCAGCTGGCTTTCTCAANNNAAAAAAANN
  NNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAACTCGACTCTAAATGGCGGCCGGGGTCATAGCTGTTTCCTGAACAAACCCC
  GGGTGGCATCCTTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAAGTGCCACTCCA
  GGGCCCACCAGCCTTGTCCTAATAAATTAAGTGCATCATTTTGCTGACCAGGNGTCCTTT
  AAAATAAGGGTGAGGGGGGGGGGGTTGGGAACAAGGGCCAATTGGGAACACACCGTAGGC
  CTGGGGGGTCTATGGGGACCAGCTGGNATGCATGCCACATCTGGCTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000991 unedited
  GACCTTTTGCCGACTGACTTTCTATGCTCTGGGGGGCAGGGAGGTCTCACGTAGCTCTTG
  GTGGGGCGGGTCCGCTTCCTCTTCACCATCACAGGCTTCTGGCTGCGCATGATGGCGCTG
  GCCCTGCGGATGGCTGCCATGCGCACGTCGGGGCGGTACTTGTTCTTGCGGATCATGTGT
  CTGATGCTGCTGAGCGTGGCGCGAGCATTCTTGTTGATGGTGGTCCGCACATAGGAGGTG
  GCAGGCTTCCGCTGGCCGGATCTCCGCTTAATGACCACCACGACACCTTTGCCGTCGGCT
  GCCGGCTCCACGCCCACAGTCTTGCGGTGAATCAGTCCGTTGTAGCGGAAGGAATTGCGG
  GCCTTCAAGTTATTGGGCTCAGTGCTGTAGGTCTGCTTATTCCTCTTGATCAGGAAACTG
  GAGCAGTTCCGCACGACCATCCATTGCAGATGCGCAGACATGGCGGCGGCTCCCTCCGCA
  GCGGCGACCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTG
  ACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCAT
  TTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCA
  AAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG
  CTATCCACGCCCATTGGTGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAA
  TACGTANATGTACTGCCAAGTANGAAAGTCCCCGTAAGTCATGTACTGGGCATAATGCCA
  GGCGGGCCATTTACCGTCATTGACGTCCAATAGGGGCCGGACTTGGCATATGATACACTT
  GATGTACTGCCAAGTGGGCAGTTTACCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000991
• Insert Size: 580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000991.3, NP_000982.2
• RefSeq Size: 4243 bp
• RefSeq ORF: 414 bp
• Locus ID: 6158
• UniProt ID: P46779
• Cytogenetics: 19q13.42
• Domains: Ribosomal_L28e
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]
  Transcript Variant: This variant (2) lacks a stop-codon-containing segment in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) has a distinct and shorter C-terminus when compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic and transcript sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.